1.购买我公司任意ELISA试剂盒指标享受免费代测，并提供技术指导服务！

二、实验材料与试剂配制：

1. 仪器与材料：酶标仪（使用前预热30分钟），微量加液器、吸头、蒸馏水或去离子水，滤纸。

2. 缓冲液使用：加5ul  的缓冲液于50ul  的样品中，如果样品量不够或者不确定，缓冲液和样品的混合比例不要小于1:10即可混匀，静置1小时备用（如果标本是血清或者血浆，此步骤忽略）。

3. 洗液的配制：按1:100的比例配制洗液备用。

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人细胞周期素D1(Cyclin-D1)ELISA试剂盒现货】三. 仔细阅读说明书

1.检查试剂盒标签上的有效期。如果超过有效期，请勿使用。

2. 按说明书确定所有试剂齐全（数量、体积）

3. 标本制备要规范，每份标本体积要按2-3个复孔以上的量制备（贮存），尽量分装做备份。

4.短期内无法实验者，注意低温保存

5.准备好所有实验额外所需物品，（比如移液器，试管，清洗器，酶标仪）

6. 按说明书将所用试剂平衡至室温，根据检测标本数量确定所需试剂的量

7.检查试剂盒内每种试剂的贮存条件，保证所有试剂均按照试剂盒内说明书推荐的条件存储。 【人细胞周期素D1(Cyclin-D1)ELISA试剂盒现货】

8.检查不稳定或者变质的试剂溶液（比如沉淀或变色）。有些试剂，比如标准品稀释液或者检测稀释液，可能在设计时就含有沉淀物。所有试剂在滴入酶标板之前，必需充分混匀。而由于沉淀物的特性，在加入酶标板之前，必需不停搅拌。

9.保证充分的孵育时间和温度。

10.切勿使用不同生产批号的试剂取代现有试剂或混合现有试剂。

11.在混合或溶解蛋白溶液时，避免泡沫产生。

12.在实验开始之前，安排好实验流程。在实验开始之前，清洁工作台。

13.若有问题，应与我公司或代理商的技术支持联系

【人细胞周期素D1(Cyclin-D1)ELISA试剂盒现货】四、客户须知

齐一生物销售：021－6034 8496；181214 53965；173021 04490

1.在收集标本前都必须有一个完整的计划，必须清楚要检测的成份是否足够稳定。我们提倡新鲜标本尽早检测，对收集后当天就进行检测的标本，及时储存在4℃备用，如有特殊原因需要周期收集标本，请造模取材后，将标本及时分装后放在-20℃或-70℃条件下保存。因冰室与室温存在一定温差，蛋白极易降解，直接影响实验质量，所以避免反复冻融。部分标本也可加入适当防腐剂，激素类标本需添加抑肽酶。 

2.试剂盒代测标本的客户取材前须向我司销售人员索要说明书，具体操作注意事项请与我司技术人员沟通。

3.液体类标本：标本必须为液体，不含沉淀。包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清、组织匀浆等。

4.购买前请提前告诉我们：您样本的种属、样本数量、待测指标及其它特殊要求。

【人细胞周期素D1(Cyclin-D1)ELISA试剂盒现货】五、代测数据详细内容

1、ELISA标准曲线；

2、实验具体详细步骤；

3、完整的实验报告（试剂耗材，实验方法，实验结果及结果说明）；

4、实验进行阶段，我公司客服人员会及时向您汇报实验进程。

HH   人肝细胞         Human Hepatocytes        2 x 10^6 cells/vial                                

HHSteC    人肝星形细胞         Human Hepatic Stellate Cells  5 x 10^5 cells/vial                                

HGBF        人胆囊成纤维细胞         Human Gallbladder Fibroblasts        5 x 10^5 cells per 1ml                                  

HSEC         人脾血管内皮细胞         Human Splenic Endothelial Cells      5 x 10^5 cells/vial                                

HSF  人脾成纤维细胞     Human Splenic Fibroblasts       5 x 10^5 cells/vial                                

HCMEC     人心脏微血管内皮细胞         Human Cardiac Microvascular Endothelial Cells      5 x 10^5 cells/vial                                

HCAEC      人冠状动脉内皮细胞     Human Coronary Artery Endothelial Cells        5 x 10^5 cells/vial                                

HAEC        人主动脉内皮细胞         Human Aortic Endothelial Cells        5 x 10^5 cells/vial                                 【人细胞周期素D1(Cyclin-D1)ELISA试剂盒现货】

HASMC     人主动脉平滑肌细胞     Human Aortic Smooth Muscle Cells          5 x 10^5 cells/vial                                

HAF  人主动脉成纤维细胞     Human Aortic Fibroblasts         5 x 10^5 cells/vial                                

HCM         人心肌细胞     Human Cardiac Myocytes        5 x 10^5 cells/vial                                

HCM-a      人心肌细胞－－成人  Human Cardiac Myocytes-adult       5 x 10^5 cells/vial                                

HCF  人心脏成纤维细胞         Human Cardiac Fibroblasts      5 x 10^5 cells/vial                                

HCF-av      人心脏成纤维细胞－－成人心室       Human Cardiac Fibroblasts-adult ventrical      5 x 10^5 cells/vial                                

HCF-aa     人心脏成纤维细胞－－成人心房       Human Cardiac Fibroblasts-adult atrial   5 x 10^5 cells/vial                                

HCF-a        人心脏成纤维细胞－－成人       Human Cardiac Fibroblasts-adult   5 x 10^5 cells/vial                                

HCF-fa      人心脏成纤维细胞－－胎儿心房       Human Cardiac Fibroblasts-fetal atrial         6 x 10^5 cells/vial                                

HPcF         人心包成纤维细胞         Human Pericardial Fibroblasts         5 x 10^5 cells/vial                                

HCEpiC     人角膜上皮细胞     Human Corneal Epithelial Cells         5 x 10^5 cells/vial                                

HK    人角膜细胞     Human Keratocytes          5 x 10^5 cells/vial                                

HREC        人视网膜内皮细胞         Human Retinal Endothelial Cells      5 x 10^5 cells/vial                                

HRPEpiC   人视网膜色素上皮细胞         Human Retinal Pigment Epithelial Cells  5 x 10^5 cells/vial                                

HLEpiC      人晶状体上皮细胞         Human Lens Epithelial Cells     5 x 10^5 cells/vial                                

HIPEpiC    人虹膜色素上皮细胞     Human Iris Pigment Epithelial Cells 5 x 10^5 cells/vial                                 【人细胞周期素D1(Cyclin-D1)ELISA试剂盒现货】

HConF       人结膜成纤维细胞         Human Conjunctival Fibroblasts       5 x 10^5 cells/vial                                

HNPCEpiC         人无色素睫装上皮细胞         Human Non-Pigment Ciliary Epithelial Cells     5 x 10^5 cells/vial                                

HTMC       人小梁网细胞         Human Trabecular Meshwork Cells 5 x 10^5 cells/vial                                

HOCF        人眼脉络膜成纤维细胞         Human Ocular Choroid Fibroblasts  5 x 10^5 cells/vial                                

HConEpiC 人结膜上皮细胞     Human Conjunctival Epithelial Cells          5 x 10^5 cells/vial                                

HVCEC      人绒毛膜毛细血管内皮细胞         Human Villus Capillary Endothelial Cells  5 x 10^5 cells/vial                                

HAmEpiC 人羊膜上皮细胞     Human Amniotic Epithelial Cells      5 x 10^5 cells/vial                                

HVT 人绒毛膜滋养层细胞     Human Villous Trophoblasts    1 x 10^6 cells/vial                                

HVMF       人绒毛间充质成纤维细胞     Human Villous Mesenchymal Fibroblasts         5 x 10^5 cells/vial                                

9238610  Collet chuck               CNY                             

9238479  Motor, syringe, RapidPlate               CNY                             

9238152  Elution Microplate Adapter 8000, SBS              CNY                             

9237932  Syringe, 100µl           CNY                             

9240663  Nut, Flangeless,1/4-28, Black, AP              CNY                             

9240634  Power Supply, Main PCB, Aztec, AP196/98                CNY                             

9240630  Power Supply, 5VDC 1.5 AMP, AP96                  CNY                             

9240593  Unload Valve, Pump, Pressure, AP196/98                  CNY                             

9240562  Fuse, PCB, 315, Eppendorf, AP196/98                CNY                             

9240536  Motor Assy, SiNug, 17, All, AP96/196                 CNY                             

9240514  CD R/W, AP196/98             CNY                             

9240504  PC-104, AP98            CNY                             

9240346  Autopure, Full Agreement       "Repair service delivery: onsite

Service response time (repair): 48 hours/2 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"        CNY                             

9240098  Re-Installation, BR EZ1    The Re-Installation, BR EZ1 facilitates quick implementation of system back into lab routine by minimizing  instrument set-up phase. It covers hardware ａnd software check of instrument ａnd provides user with operator refesher overview.  Re-installation includes labor ａnd travel costs. Re-installation performance process is documented on a GMP/GLP-compliant Field Service document.          CNY                             

9239852  Foot bumpers,  v2.0 (4)          CNY                              【人细胞周期素D1(Cyclin-D1)ELISA试剂盒现货】

9019944  Cable, P-axis, wires, 8 ch. , C18                 CNY                             

9238880  Bearings up. & low, BS96                   CNY                             

9238836  BioSprint96, Basic Agreement, no PM    "Repair service delivery: onsite

Service response time (repair): 5 business days

Periodic inspection/maintenance: not included

Inspection/maintenance delivery: not applicable

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: not applicable"        CNY                             

9241211  Full Agreement Depot Repair, DML2000, 1y  "The Full Agreement Depot Repair, DML2000 provides full service coverage for the DML 2000 luminometer. The service is conducted as an instrument exchange service.

All shipping, labor ａnd repair part costs related to an instrument exchange are included for 1 year. Instrument exchange time is 2 - 3 working days."          CNY                             

9241191  Magnet, tool, QX              CNY                             

9238825  Spare bucket for plate rotor             CNY                             

9238815  Rect. carrier for 12 tubes 10 ml w/ cap            CNY                             

9019472  TAPE SPACER CLEAR GS             CNY                             

9019464  UV tube, Cxx             CNY                             

9243650  Comprehen Valid., EZ1/QIAcube/QIAsymph    """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   The Customer is expected to have all materials on-hand 1 week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """     CNY                             

9237333  Install, BR plus 3 integr. w. scheduling     Installation ａnd training, BioRobot System including three approved integration devices with scheduling   CNY                             

9237328  Upgrade, BR & 2 ext. devices w. sched.  Upgrade BioRobot with two approved integration devices using scheduling software  CNY                             

9237173  Shield, V-housing               CNY                             

9233208  Tubing, liquid system 2.5/1.5             CNY                             

9237155  V-Housing with Sample Needle                  CNY                             

9237150  Heater Top                CNY                              【人细胞周期素D1(Cyclin-D1)ELISA试剂盒现货】