检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供*优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【人粒细胞巨噬细胞集落刺激因子抗体(GM-CSFAb)ELISA试剂盒现货】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人粒细胞巨噬细胞集落刺激因子抗体(GM-CSFAb)ELISA试剂盒现货】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在、第二孔中分别加标准品100μl,然后在、第二孔中加标准品稀释液50μl,混匀；然后从孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【人粒细胞巨噬细胞集落刺激因子抗体(GM-CSFAb)ELISA试剂盒现货】

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品*终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果. 【人粒细胞巨噬细胞集落刺激因子抗体(GM-CSFAb)ELISA试剂盒现货】

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请*后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

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QY-14812 Anti-IL-23(Interleukin-23)白介素-23抗体                                                    

QY-14822 Anti-IL-4(Interleukin-4)白介素4抗体                                                  

QY-14832 Anti-IL-6 (Interleukin-6) Human白介素6                                                     

QY-14842 Anti-IL-6 (Interleukin-6) mouse、rat白介素6抗体                                                    

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QY-14862 Anti-IL-8（Interleukin-8）rat mouse白介素8抗体                                                   

QY-14872 Anti-ILK-1 (Integrin-linked protein kinase 1)整合素连接激酶-1抗体                                                  

QY-14882 anti-ING1/p33(inhibitor of growth gene 1)生长抑制因子基因1抗体                                                

QY-14892 Anti-Inhibin α抑制素α抗体                                                      

QY-14902 Anti-Insulin胰岛素抗体                                               

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QY-14932 Anti-Integrin α3β1整合素α3β1抗体                                                  

QY-14942 Anti-Integrin α4β7/LPAM-1 (Lymphocyte Peyer’s patch Adhesion Molecule 1/Integrin alpha4,beta7)整合素α4β7抗体                                                        【人粒细胞巨噬细胞集落刺激因子抗体(GM-CSFAb)ELISA试剂盒现货】

QY-14952 anti-Integrin avb3整合素avb3抗体                                                   

QY-14962 Anti-Integrin αE2/CD103整合素αE2抗体                                                       

QY-14972 Anti-IQGAP1支架蛋白抗体                                                 

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QY-15002 Anti-IRS-2胰岛素受体底物-2抗体                                                      

QY-15012 Anti-IRS-3胰岛素受体底物-3抗体                                                      

QY-15022 Anti-IRS-4胰岛素受体底物-4抗体                                                      

QY-15032 Anti-ISR（Insulin Receptor）胰岛素受体抗体                                                    

QY-15042 Anti-ISR-α （Insulin Receptor-α）胰岛素受体-α抗体                                                  

QY-15052 Anti-ISR-β （Insulin Receptor-β）胰岛素受体-β抗体                                                  

QY-15062 Anti-ITG-α/ CD49d整合素α4抗体                                                   

QY-15072 Anti-ITG-β1/CD29（integrin β1）粘附分子整合素β1抗体                                                 

QY-15082 Anti-ITG-α5/CD49e（Integrin α5）粘附分子整合素α5抗体                                                       

QY-15092 Anti-ITG-β3/CD61(Integrin β chain,β3 precursor)整合素-β3抗体                                                      

QY-15102 Anti-JNK1/3 (c-Jun amino-terminal kinase 1/3)c-Jun氨基末端激酶1/3抗体                                                  【人粒细胞巨噬细胞集落刺激因子抗体(GM-CSFAb)ELISA试剂盒现货】

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QY-15122 Anti-Ki-67Ki-67 Antigen抗体                                               

9011433  BGR Z-Motor P1300 Spez. MWG              CNY                             

9011422  Tip Rack Holder, RCS1               CNY                             

9243119  Green Laser Contr. Coherent 115M-13, LC               CNY                             

9243556  HC2, Full Agreement        "Service response time (on-site repair):not applicable

Periodic inspection/maintenance: yes, 1/agreement year

Cost coverage depot repair (parts, labor, packaging, shipping): yes, full coverage

For defect DML, after customer calling QIAGEN technical service hotline before 12 am:

- A special package containing a replacement unit shall be sent to the customer within 2 working days.

- Customer shall send the defect unit to QIAGEN Regional Repair Center following the instructions attached with the packaging.  

For DML owned by customer, QIAGEN Regional Repair Center will send back the repaired original unit to customers within 7-10 working days.

PC, Monitor, Heater, Shaker, Washer, Printer ａnd Votexer:

- A new unit shall be sent to customer within  7-10 working days at no extra cost

- Customer shall send the defect unit to QIAGEN for disposal

- On-site installation of PC ａnd application software is not covered by the agreement

The Preventive Maintenance (PM) service ensures the correct operation of the equipment. During the PM, all the instrument modules are inspected by a qualified QIAGEN service technician. The Preventive Maintenance is performed under the manufacturer guidelines ａnd documented according to GLP / GMP"    CNY                             

101.901-24       #HLA-DQB1*0602; DQA1*0102 incl.Taq (24)           CNY                             

9241033  #Tube insert, 11 mm, sample carr            CNY                             

9242760  #Warranty PLUS 1, Prem/RRL, 1PM,QSYM RGQ              CNY                             

677491     ipsogen NPM1 mut A gDNA MutaQuant Stds          CNY                              【人粒细胞巨噬细胞集落刺激因子抗体(GM-CSFAb)ELISA试剂盒现货】

9011164  Roller, deflection, X-drive, BR8000           CNY                             

9010980  BGR 1-er Shaker PK80 (1177)           CNY                             

133215     NeXtalStock MMT pH 9.0 (200)                 CNY                             

301005     Attractene Transfection Reagent (1 ml)           CNY                             

9012675  Peristaltic Pump, 4 channel (A04897)               CNY                             

9012671  Cable, PCON98 to release valve, BR8000                  CNY                             

9002029  Rotor-Gene Q MDx 5plex, PrioPLUS         "Rotor-Gene Instrument

Installation of Rotor-Gene & Introductory training

Warranty extension to 3 yrs

Regional Repair Center service, 7-10 day turn around time

Loaner available in 2 days*

Annual Inspections (1PM per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

9002019  Rotor-Gene Q MDx 2plex HRM, PrioPLUS       "Rotor-Gene Instrument

Installation of Rotor-Gene & Introductory training

Warranty extension to 3 yrs

Loaner available in 2 days*

Annual Inspections (1 onsite PM per yearr)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

9002002  Rotor-Gene Q MDx 2plex Platform          CNY                             

970540     UGT1A1 Pyro Kit (24)                CNY                             

293025     mericon Corn (96)             CNY                             

293015     mericon Soy Kit (96)                   CNY                             

929525     QX Alignment Marker 50bp/500bp (1.5 ml)              CNY                             

206241     Type-it Microsatellite PCR Kit (70)   "The Type-it Microsatellite PCR Kit is developed for fast ａnd reliable genotypic analysis of humans, animals, plants, ａnd bacteria using

microsatellites, STR, or VNTR markers without the need for lengthy optimization procedures

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 Microsatellite assay development without optimization

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291524     mericon Quant MON 810 (48)          CNY                             

9001904  QIAgility HEPA/UV (PC) PrioPLUS     "QIAgility HEPA/UV (PC)Instrument

Installation of QIAgility instrument & Introductory training

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite pipetting head service ａnd inspection per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                              【人粒细胞巨噬细胞集落刺激因子抗体(GM-CSFAb)ELISA试剂盒现货】

9001899  QIAxtractor, UV/HEPA (PC) Priority QIAxtractor, UV/HEPA (PC) Priority includes the QIAxtractor instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service ａnd inspection is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor ａnd travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.        CNY                             

133124     NeXtalStock TRIS pH 8.5 (200)                   CNY                             

965942     QIAamp Investigator BioRobot Kit (12)            CNY                             

133191     NeXtalStock D-(+)-Sucrose (200)               CNY                             

133184     NeXtalStock Citric acid pH 3.6 (200)                  CNY                             

9018472  Set, O-rings (10), QIAC              CNY                             

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