检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供*优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【人抗磷脂酰丝氨酸抗体(APSA)ELISA试剂盒现货】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

【人抗磷脂酰丝氨酸抗体(APSA)ELISA试剂盒现货】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在、第二孔中分别加标准品100μl,然后在、第二孔中加标准品稀释液50μl,混匀；然后从孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品*终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

【人抗磷脂酰丝氨酸抗体(APSA)ELISA试剂盒现货】注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果.

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请*后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

【人抗磷脂酰丝氨酸抗体(APSA)ELISA试剂盒现货】计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

QY-00710 NPY （ Neuropeptide Y）神经肽 Y（抗原）               0.5mg/6501mg/1100                                  

QY-02220 NR2B （Glutamate receptor）谷氨酸受体（抗原）           0.5mg/6501mg/1100                                  

QY-01600 NT-3神经生长因子-3（抗原）                0.5mg/6501mg/1100                                  

QY-01580 NT-4,NT-5神经生长因子4/5（抗原）             0.5mg/6501mg/1100                                  

QY-00730 NTN （Neurturin） 神经生长因子（抗原）                0.5mg/6501mg/1100                                  

QY-01780 Nurr1核受体相关因子-1（抗原）                  0.5mg/6501mg/1100                                  

QY-03720 CD73 (5´-nucleotidase cytosolicⅡ)胞浆-5´-核苷酸酶-Ⅱ 抗原              0.5mg/6501mg/1100                                  

QY-02400 OGP (Ostegenic Growth Peptide)成骨生长肽 (骨生长激素肽)(多肽)           0.5mg/6501mg/1100                                 

QY-00190 OPN骨桥蛋白（多肽抗原）                    0.5mg/6501mg/1100                                  

QY-00330 P53肿瘤抑制基因（抗原）            0.5mg/6501mg/1100                                  

QY-01900 PACAP-27/38腺苷酸环化酶激活肽-27/38（抗原）                  0.5mg/6501mg/1100                                  

QY-02000 PACAP-38腺苷酸环化酶激活肽-38（抗原）                  0.5mg/6501mg/1100                                  

QY-01970 PACAP/VIP receptor腺苷酸环化酶激活肽/血管活性肠肽受体（抗原）             0.5mg/6501mg/1100                                  【人抗磷脂酰丝氨酸抗体(APSA)ELISA试剂盒现货】

QY-01980 PACAP receptor-I腺苷酸环化酶激活肽受体（抗原）                   0.5mg/6501mg/1100                                  

QY-03180 PADI4 (HL-60 PAD)(Protein-arginine deiminase type IV，PADI 4，PAD I4 )关节炎相关基因                  0.5mg/6501mg/1100                                    

QY-01960 PDGF-A血小板源性生长因子-1（抗原）               0.5mg/6501mg/1100                                  

QY-01850 PDGF-B血小板源性生长因子-B（抗原）              0.5mg/6501mg/1100                                  

QY-02310 PDGF-R-A血小板源性生长因子受体-A（抗原）                    0.5mg/6501mg/1100                                  

QY-02320 PDGF-R-B血小板源性生长因子受体-B（抗原）                    0.5mg/6501mg/1100                                  

QY-02350 PMP-22(Peripheral Myelin Protein)外周髓鞘蛋白-22 多肽            0.5mg/6501mg/1100                                  

QY-01280 PI3K磷脂酰肌醇激酶（抗原）               0.5mg/6501mg/1100                                  

QY-02340 PLGF （Placenta growth factor）小鼠胎盘生长因子（多肽抗原）           0.5mg/6501mg/1100                                  

QY-02800 PLGF （Placenta growth factor）胎盘生长因子（多肽抗原）           0.5mg/6501mg/1100                                  

QY-02810 PLGF （Placenta growth factor fragment）(human)人胎盘生长因子（多肽抗原）                   0.5mg/6501mg/1100                                  

QY-01330 PP-1蛋白磷酸酯酶-1（抗原）                0.5mg/6501mg/1100                                  

QY-02500 PPAR-delta (peroxisome proliferator-activated receptor)D型-过氧化酶活化增生受体(多肽）                    0.5mg/6501mg/1100                                  

QY-00290 PP-2A （protein phosphatase 2A）蛋白质磷酸酶-2A（抗原）            0.5mg/6501mg/1100                                  

QY-00290 PP-2A （protein phosphatase 2A）蛋白质磷酸酶-2A（抗原）            0.5mg/6501mg/1100                                  

QY-00300 PP-2B alpha 1 蛋白磷酸酯酶-2B 催化亚单位（抗原）                0.5mg/6501mg/1100                                   【人抗磷脂酰丝氨酸抗体(APSA)ELISA试剂盒现货】

QY-01110 PR （Progestogerone receptor）孕激素受体（抗原）                0.5mg/6501mg/1100                                  

QY-00240 PS-1（NT），Presennillin-1（S182）早老素蛋白-1（抗原）                  0.5mg/6501mg/1100                                  

QY-00250 PS-1，Presennillin-1（CT）早老素蛋白-1（S182）                0.5mg/6501mg/1100                                  

QY-08030 BRCA1（Breast cancer susceptbility gene 1）(mouse rat)乳腺癌易感基因1抗原(小鼠 大鼠)                   0.5mg/6501mg/1100                                  

9240705  O-ring Kit, Capper, Spindle, AP                  CNY                             

9240693  Actril Sterilant & Test Strips, AP                CNY                             

9243653  Comprehen Valid., Other Extraction Sys """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   The Customer is expected to have all materials on-hand 1 week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """     CNY                             

386516     Investigator STR GO! Lysis Buffer (200)            CNY                             

208256     QuantiNova Probe PCR Kit (2500)             CNY                             

208254     QuantiNova Probe PCR Kit (500)               CNY                             

180984     GeneRead Adapter I Set 12plex (72)                 CNY                             

614035     careHPV Test, SFDA (96-test)           CNY                             

282605     virotype Influenza A RT-PCR Kit (96)                  CNY                             

272703     pigtype PRRSV Ab (5)                 CNY                              【人抗磷脂酰丝氨酸抗体(APSA)ELISA试剂盒现货】

273003     pigtype Salmonella Ab (5)                  CNY                             

280377     virotype BVDV RT-PCR Kit (480)                CNY                             

9018462  Marker Kit, QC tool, QX            CNY                             

800372     Shipping, Postage, Packaging (express)            CNY                             

800373     Data Carrier              CNY                             

9019521  ZLV ADAPTER KIT M&N 8 STRIP                CNY                             

9019595  ZLV Bush, tip stripper, Cx8                 CNY                             

9019935  Cable harness, X-axis 8 core, CQX              CNY                             

9020596  Tape Foam,045X750 Double Stick,DML 3000           CNY                             

9017843  Tool, cable, communication, RS232, QIAC                CNY                             

9013401  Cable set, cooling system, 90/120             CNY                             

9234873  ZLVPipetting Station for 2 microtit.                   CNY                             

9013395  Tip disposal bags               CNY                             

9013391  Tubing set, PVC, BR9604          CNY                             

9013268  Cable, card-X to solenoid valve, 24V                  CNY                             

9013222  Holder, tube, STS, single, 11 ml (30), 0             CNY                              【人抗磷脂酰丝氨酸抗体(APSA)ELISA试剂盒现货】

9013204  Transportation kit, BR8000               CNY                             

9011759  Reagent holder, 4-tube 13ml  Holder for accommodating 4 microcentrifuge tubes (13 ml) on the worktable; must be used with the reagent holder tray   CNY                             

9011672  Reader Abdeckung spez. GSG (9194)                CNY                             

132605     NeXtal Stock Sodium ac. H2O (50)   1.6M Sodium acetate trihydrate - 50mL (4 x 12,5 ml tube)    CNY                             

800141     Investigation grade sequencing, w/ prep         "Single-stranded sequencing of an unknown sequence

QIAGEN DNA template preparation

Design ａnd synthesis of walking primers

Data assembly ａnd sequence alignment

Accuracy >99%"       CNY