【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【人抗神经元核抗体2型/抗Ri抗体(ANNA-2/Ri)ELISA试剂盒现货】样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【人抗神经元核抗体2型/抗Ri抗体(ANNA-2/Ri)ELISA试剂盒现货】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

【人抗神经元核抗体2型/抗Ri抗体(ANNA-2/Ri)ELISA试剂盒现货】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【人抗神经元核抗体2型/抗Ri抗体(ANNA-2/Ri)ELISA试剂盒现货】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

 QY-25213      Anti-FGF1/FITC荧光素标记酸性成纤维细胞生长因子           0.5ml/1680                        

 QY-25214      Anti-FoxP3/FITC荧光素标记叉头蛋白3-T细胞转录因子抗体IgG               0.5ml/1500                        

 QY-25215      Anti-GFAP/FITC荧光素标记胶质纤维酸性蛋白抗体IgG               0.5ml/1680                        

 QY-25216      Anti-GFP/FITC (Green Fluorecent protein)荧光素标记兔抗绿色荧光蛋白抗体IgG              0.5ml/1500                        

 QY-25217      Anti-GLP-1(7-36)/FITC荧光素标记兔抗胰高血糖素样肽-1抗体IgG            0.5ml/1860                        

 QY-25218      Anti-HSP-27/FITC荧光素标记HSP-27蛋白抗体IgG              0.5ml/1500                        

 QY-25219      Anti-HSP-60/FITC荧光素标记热休克蛋白-60蛋白抗体IgG           0.5mg/1500                       

 QY-25220      Anti-HSP-70/FITC荧光素标记HSP-70蛋白抗体IgG              0.5ml/1500                        

 QY-25221      Anti-HSP-90α/FITC荧光素标记HSP-90α蛋白抗体IgG               0.5ml/1500                        

 QY-25222      Anti-HSP-90β/FITC荧光素标记HSP-90β蛋白抗体IgG               0.5ml/1500                        

 QY-25223      Anti-IASPP/FITC荧光素标IASPP蛋白抗体IgG              0.5ml/1500                         【人抗神经元核抗体2型/抗Ri抗体(ANNA-2/Ri)ELISA试剂盒现货】

 QY-25224      Anti-IGF-I/FITC荧光素标记标记胰岛素样生长因子-I抗体IgG            0.5ml/1500                        

 QY-25225      Anti-Insulin/FITC荧光素标记标记胰岛素抗体IgG           0.5ml/1500                        

 QY-25226      anti-Integrin avb3/FITC荧光素标记整合素avb3抗体IgG               0.5ml/1860                        

 QY-25227      Anti-Insulin/HRP辣根过氧化物酶标记胰岛素抗体IgG            0.1ml/800                          

 QY-25228      Anti-ISR-α /FITC(Insulin Receptor-α)荧光素标记胰岛素受体抗体             0.5ml/1500                        

 QY-25229      Anti-ISR-α protein/FITC荧光素标记胰岛素受体-α蛋白抗体IgG               0.5ml/1500                        

 QY-25230      Anti-ISR-α protein/RBITC罗丹明标记胰岛素受体-α 抗体IgG           0.5ml/1860                        

 QY-25231      ITG-α/FITC荧光素标记整合素α4抗体IgG           0.5ml/1500                        

 QY-25232      Anti-Leptin/FITC荧光素FITC标记瘦素抗体IgG             0.5ml/1500                        

 QY-25233      Anti-LPAM-1/FITC（Integrin alpha4,beta7）荧光素标记整合素α4β7抗体IgG          0.5ml/1500                        

 QY-25234      Anti-MMP-9/FITC荧光素标记基质金属蛋白酶-9蛋白抗体IgG             0.5ml/1500                        

 QY-25235      Anti-MRP2/FITC荧光素标记多药耐药相关蛋白2抗体           0.5ml/1500                        

 QY-25236      Anti-NGFR/FITC荧光素标记神经生长因子受体抗体gG(标记抗体)             0.5ml/1500                        

 QY-25237      MMP-7/FITC荧光素标记基质金属蛋白酶-7抗体IgG             0.5ml/1500                        

 QY-25238      Anti-NIK/FITC荧光素标记NFkB诱导的激酶抗体IgG            0.5ml/1500                        

 QY-25239      Anti-OVA/HRP辣根过氧化物酶标记兔抗鸡卵白蛋白抗体IgG               0.1ml/480                           【人抗神经元核抗体2型/抗Ri抗体(ANNA-2/Ri)ELISA试剂盒现货】

 QY-25240      Anti-PCNA/FITC荧光素标记兔抗增值细胞核抗原抗体IgG           0.5ml/1500                        

 QY-25241      Anti-PKB/FITC荧光素标记蛋白激酶B抗体IgG              0.5ml/1500                        

 QY-25242      Anti-P-GP/FITC荧光素标记P-糖蛋白抗体IgG         0.5ml/1500                        

 QY-25243      Anti-PTP-1B/FITC荧光素标记PTP-1B蛋白抗体IgG              0.5ml/1680                        

 QY-25244      Anti-Survivin/RBITC红色荧光素罗丹明标记 Survivin 蛋白抗体(标记抗体)         0.5ml/1680                        

 QY-25245      Anti-Resistin/FITC荧光素标记抵抗素抗体IgG         0.5ml/1500                        

218422    Mouse miScript Assay 96 SetVXX.X (20)           CNY                           

9018306  EZ1 Advanced DSP Virus Card            CNY                           

800349    BAC End-Sequencing, w/o prep           CNY                           

9015302  Stopper, reagent tub, right, M48           CNY                           

9015295  Supporters, cap seal, M48 (6)              CNY                           

9015292  O-rings, nozzles, M48 (6)            CNY                           

9016757  Appl. Pack Sequencing for Univ.PF             CNY                           

9015321  Belt, P-motor, M48        CNY                           

133133    NeXtalStock Sodium ac. pH 4.6 (200)         CNY                           

9243231  RCS, Full Agreement     "

- Service response time RCS: 5 working days

- Inspection/maintenance delivery: yes, included; 1 /agreement year

- Cost coverage for repair parts: yes, included

- Cost coverage for labor: yes, included

- Cost coverage for travel: yes, included

- Replacement system provision for DML: yes, included

- Transportation cost coverage for DML: yes, included

For defect units, after customer calling QIAGEN technical service hotline before 12 am:

RCS:

- QIAGEN shall dispatch a specialist to repair the defect instrument at customer site within 48 Hrs / 2 working days.

DML:

- A special package containing a replacement unit shall be sent to the customer within 48 Hrs / 2 working days.

- Customer shall send the defect unit to QIAGEN Regional Repair Center following the instructions attached with the packaging.  

Note: for DML owned by customer, QIAGEN Regional Repair Center will send back the repaired original unit to customers within 7-10 working days.

PC, Monitor, Heater, Shaker, Washer, Printer ａnd Votexer:

- A new unit shall be sent to customer within  7-10 working days at no extra cost

- Customer shall send the defect unit to QIAGEN for disposal

- On-site installation of PC ａnd application software is not covered by the agreement

The Preventive Maintenance (PM) service ensures the correct operation of the equipment. During the PM, all the instrument modules are inspected by a qualified QIAGEN service technician. The Preventive Maintenance is performed under the manufacturer guidelines ａnd documented according to GLP / GMP"  CNY                           

36113      Ni-NTA Magnetic Agarose Beads (6 x 1 ml) 6 x 1 ml nickel-charged magnetic agarose beads (5% suspension)     CNY                           

950207    Reagent Pack, VX  The Reagent Pack, VX, which is used together with the Virus Plasticware Pack, QIAxtractor (#950047) provides fully automated viral nucleic acid purification on the QIAxtractor. The combination of reagents ａnd plasticware enables purification of viral nucleic acids from liquid samples, with a throughput of 8-96 samples per run.  CNY                            【人抗神经元核抗体2型/抗Ri抗体(ANNA-2/Ri)ELISA试剂盒现货】

9018299  EZ1 Advanced DNA Dried Blood Card         CNY                           

9239543  Assy, XY, Slotted Optical Switch 1              CNY                           

9015299  Motor, P/M, M48          CNY                           

9015294  Plate, ｄrop catcher, M48              CNY                           

132981    NeXtal Stock Kit Cryo   24 x 10 ml tubes containing a selection of cryoprotectants  CNY                           

9016755  Appl. Pack Genotyping for Univ.PF             CNY                           

9019453  Front label incl. cutout for  P/R, HS           CNY                           

9019450  IC PIC, programmed , HS            CNY                           

9014276  Positioning plate            CNY                           

9019906  Seal - VPT Filter probes, PyroQ24VW         CNY                           

9019905  Insert VPT, PyroQ24VW             CNY                           

9018155  Sensor, bottle, waste drawer, Qsym            CNY                           

9018147  Consumable drawer, Qsym           CNY                           

9018144  Cable, heated shaker, Qsym          CNY                           

9018090  Cooling adapter, Reagent holder 1, Qsym            CNY                           

9018068  Kit, fuse, Qsym             CNY                            【人抗神经元核抗体2型/抗Ri抗体(ANNA-2/Ri)ELISA试剂盒现货】

9017851  Labware Tray        CNY                           

9017845  Shaker Adapter, 2.0 ml          CNY                           

9017818  Sensor, light barrier, gripper, QIAC              CNY