【人抗肝特异性脂蛋白抗体(LSP)ELISA试剂盒厂家】规    格：96T/48T

检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供*优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【人抗肝特异性脂蛋白抗体(LSP)ELISA试剂盒厂家】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人抗肝特异性脂蛋白抗体(LSP)ELISA试剂盒厂家】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在、第二孔中分别加标准品100μl,然后在、第二孔中加标准品稀释液50μl,混匀；然后从孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【人抗肝特异性脂蛋白抗体(LSP)ELISA试剂盒厂家】

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品*终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果. 【人抗肝特异性脂蛋白抗体(LSP)ELISA试剂盒厂家】

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请*后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

QY-L0904 异香兰素621-59-0 Isovanillin          621-59-0  20mg                          

QY-L0905 仙鹤草酚B55576-66-4   Agrimol B          55576-66-4       20mg                          

QY-L0906 细辛脂素133-04-0 (-)-asarinin        133-04-0  20mg                          

QY-L0907 小豆蔻明19309-14-9      Cardamonin     19309-14-9       20mg                           【人抗肝特异性脂蛋白抗体(LSP)ELISA试剂盒厂家】

QY-L0908 辛夷脂素31008-19-2      Fargesin   31008-19-2       20mg                          

QY-L0909 仙茅苷85643-19-2 Curculigoside   85643-19-2       20mg                          

QY-L0910 血根碱2447-54-3   Sanguinarine    2447-54-3         20mg                          

QY-L0911 柚皮甙二氢查尔酮18916-17-1      Naringin dihydrochalcone        18916-17-1       20mg                          

QY-L0912 柚皮苷10236-47-2 Naringin           10236-47-2       20mg                          

QY-L0913 芸香柚皮苷14259-46-2 Narirutin  14259-46-2       20mg                          

QY-L0914 柚皮素480-41-1      Naringenin       480-41-1  20mg                          

QY-L0915 柚皮素-7-O-葡萄糖醛酸苷     "Naringenin-7-O-glucuronide

"                 20mg                          

QY-L0916 小白菊内酯20554-84-1 Parthenolide    20554-84-1       20mg                          

QY-L0917 夏佛塔苷51938-32-0      Schaftoside       51938-32-0       20mg                          

QY-L0918 异夏佛塔苷52012-29-0 Isoschaftoside          52012-29-0       20mg                          

QY-L0919 香草酸121-34-6      Vanillic acid     121-34-6  20mg                          

QY-L0920 香草醇498-00-0      4-hydroxy-3-methoxybenzylalcohol(Vanillicalcohol)         498-00-0  20mg                          

QY-L0921 香紫苏醇515-03-7 Sclareol    515-03-7  20mg                          

QY-L0922 香紫苏内酯564-20-5      Clareolide         564-20-5  20mg                          

QY-L0923 香蜂草苷 14259-47-3    Didymin   14259-47-3       20mg                           【人抗肝特异性脂蛋白抗体(LSP)ELISA试剂盒厂家】

QY-L0924 异乌药内酯728-61-0      linderalactone 728-61-0  20mg                          

QY-L0925 α-香附酮473-08-5         α-Cyperone    473-08-5  20mg                          

QY-L0926 相思子碱21339-55-9      L-Abrine   21339-55-9       20mg                          

QY-L0927 血竭素400603-95-4        Cochinchinenin        400603-95-4    20mg                          

QY-L0928 血竭素高氯酸盐125536-25-6        Dracohodin perochlorate         125536-25-6    20mg                          

QY-L0929 香荆芥酚499-75-2 5-Isopropyl-2-methylphenol     499-75-2  20mg                          

QY-L0930 缬草三酯18296-44-1      Valtrate   18296-44-1       20mg                          

QY-L0931 乙酰缬草三酯         Acevaltratum            20mg                          

QY-L0932 西瑞香素2034-69-7        Daphnoretin  2034-69-7         20mg                          

QY-L0933 雪松醇77-53-2        Cedrol       77-53-2    20mg                          

QY-L0934 辛弗林（94-07-5）1994/7/5    Synephrine       34520       20mg                          

9241217  RCS, Premium Agreement, 2PM, 1 yr      "The Premium Cover Agreement RCS, 2 PM provides full service coverage for the Rapid Capture System (on-site repair service) ａnd the associated DML, the MST II Vortexer, ａnd the PC (in case of an error the associated equipment is exchanged).

Costs for services are fully covered including shipment, travel, labor, ａnd parts. The agreement also includes two annual on-site preventive maintenance visits, with costs for labor ａnd parts covered. With a Premium Agreement, there is a priority response time for the on-site service of 24 hours (1 working day)."        CNY                             

9238818  Rect. carrier for 4 Falcon tubes 15ml                CNY                             

9233335  Valve, pressure relief, dispenser,BR8000                   CNY                             

9233291  Magnet, stop position, cover, BR8000              CNY                             

9237715  BR M48, Full Agreement The Full Agreement M48 provides full service coverage for the BioRobot M48. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).  CNY                              【人抗肝特异性脂蛋白抗体(LSP)ELISA试剂盒厂家】

9237713  EZ1, Installation       The Installation, BR EZ1 facilitates the quick implementation of the BioRobot EZ1 or EZ1 Advanced into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the BioRobot EZ1 or EZ1 Advanced instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.        CNY                             

9233165  PCB, ReaderPowerSupply, X-main             CNY                             

9237159  Laser Driver, Uniphase              CNY                             

9237149  XYP Carrier                CNY                             

9237116  Upgrade App., Ni-NTA Superflow              CNY                             

9237101  Upgrade, BR plus 1 ext. device        Upgrade BioRobot with one approved integration device      CNY                             

9236365  Filter Fan XYP            CNY                             

9232932  Reader(VIS), with auto plate positioning                   CNY                             

9232931  Reader Verdrahtung Netz-Mainboard Plato7          CNY                             

9236350  Pressure Regulator, 10 Psi                 CNY                             

9236327  PMT          CNY                             

9236300  Gasket, auto vac, top BR8000 v1.00                 CNY                             

9230316  Container, SystemLiquid, 10 l            CNY                             

9230312  Power cables for 4 syringe pumps             CNY                             

9235273  Bottle, condensate, auto vac, BR8000             CNY                             

9230280  Cable, card-X to bar-code camera Denso                  CNY                              【人抗肝特异性脂蛋白抗体(LSP)ELISA试剂盒厂家】

9230058  Toothwheel, Z-Motor, P1/3 / BR3/96                  CNY                             

9234860  Tubing coupling piece for system liquid             CNY                             

9238666  Pneumatic spring, 200 Nm                CNY                             

9238661  Filter dryer                CNY                             

9238660  Temperature sensor                  CNY                             

9242029  QIAxcel, Repair Fee "Repair service delivery send to Regional Repair Center

Service response time (repair) 7-10 days turnaround time

Periodic inspection/maintenance: 0

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

9241895  QIArent&go - Rent out fee QIAsymphony AS           CNY                             

9241814  Pyro Q24, PM Agreement        An agreement for preventive maintenance provides coverage for travel, labor, ａnd parts, plus the cost of consumables required for the maintenance visit. Work carried out during the preventive maintenance visit is fully documented in accordance with GMP/GLP directives.   CNY                             

9241165  Board, smart key, QX                 CNY                             

9019855  72-well High Profile Locking Ring              CNY                             

9019833  Cable, RS232,  external, V2, QX              CNY                             

9019763  Motor, Y, V2, QX                CNY