1.购买我公司任意ELISA试剂盒指标享受免费代测，并提供技术指导服务！

二、实验材料与试剂配制：

1. 仪器与材料：酶标仪（使用前预热30分钟），微量加液器、吸头、蒸馏水或去离子水，滤纸。

2. 缓冲液使用：加5ul  的缓冲液于50ul  的样品中，如果样品量不够或者不确定，缓冲液和样品的混合比例不要小于1:10即可混匀，静置1小时备用（如果标本是血清或者血浆，此步骤忽略）。

3. 洗液的配制：按1:100的比例配制洗液备用。

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人低密度脂蛋白免疫复合物(LDL-IC)ELISA试剂盒厂家】三. 仔细阅读说明书

1.检查试剂盒标签上的有效期。如果超过有效期，请勿使用。

2. 按说明书确定所有试剂齐全（数量、体积）

3. 标本制备要规范，每份标本体积要按2-3个复孔以上的量制备（贮存），尽量分装做备份。

4.短期内无法实验者，注意低温保存

5.准备好所有实验额外所需物品，（比如移液器，试管，清洗器，酶标仪）

6. 按说明书将所用试剂平衡至室温，根据检测标本数量确定所需试剂的量

7.检查试剂盒内每种试剂的贮存条件，保证所有试剂均按照试剂盒内说明书推荐的条件存储。 【人低密度脂蛋白免疫复合物(LDL-IC)ELISA试剂盒厂家】

8.检查不稳定或者变质的试剂溶液（比如沉淀或变色）。有些试剂，比如标准品稀释液或者检测稀释液，可能在设计时就含有沉淀物。所有试剂在滴入酶标板之前，必需充分混匀。而由于沉淀物的特性，在加入酶标板之前，必需不停搅拌。

9.保证充分的孵育时间和温度。

10.切勿使用不同生产批号的试剂取代现有试剂或混合现有试剂。

11.在混合或溶解蛋白溶液时，避免泡沫产生。

12.在实验开始之前，安排好实验流程。在实验开始之前，清洁工作台。

13.若有问题，应与我公司或代理商的技术支持联系

【人低密度脂蛋白免疫复合物(LDL-IC)ELISA试剂盒厂家】四、客户须知

齐一生物销售：021－6034 8496；181214 53965；173021 04490

1.在收集标本前都必须有一个完整的计划，必须清楚要检测的成份是否足够稳定。我们提倡新鲜标本尽早检测，对收集后当天就进行检测的标本，及时储存在4℃备用，如有特殊原因需要周期收集标本，请造模取材后，将标本及时分装后放在-20℃或-70℃条件下保存。因冰室与室温存在一定温差，蛋白极易降解，直接影响实验质量，所以避免反复冻融。部分标本也可加入适当防腐剂，激素类标本需添加抑肽酶。 

2.试剂盒代测标本的客户取材前须向我司销售人员索要说明书，具体操作注意事项请与我司技术人员沟通。

3.液体类标本：标本必须为液体，不含沉淀。包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清、组织匀浆等。

4.购买前请提前告诉我们：您样本的种属、样本数量、待测指标及其它特殊要求。

【人低密度脂蛋白免疫复合物(LDL-IC)ELISA试剂盒厂家】五、代测数据详细内容

1、ELISA标准曲线；

2、实验具体详细步骤；

3、完整的实验报告（试剂耗材，实验方法，实验结果及结果说明）；

4、实验进行阶段，我公司客服人员会及时向您汇报实验进程。

QY-x61      MGC80-3胃癌细胞                                                       

QY-x62      95-D高转移肺癌细胞                                                   【人低密度脂蛋白免疫复合物(LDL-IC)ELISA试剂盒厂家】

QY-x63      BGC-823胃腺癌细胞低分化                                                        

QY-x64      BT549乳腺管癌细胞      HTB-122                                       

QY-x65      SGC-7901胃腺癌细胞                                                  

QY-x66      T-47D乳腺管癌细胞       HTB-133                                       

QY-x67      T-47D乳腺管癌细胞       HTB-133                                       

QY-x68      TE-11食管癌细胞                                                

QY-x69      SK-BR-3乳腺腺癌细胞   HTB-30                                         

QY-x70      SK-BR-3乳腺腺癌细胞   HTB-30                                         

QY-x71      TE-10食管癌细胞                                                

QY-x72      ZR-75-30乳腺癌细胞     CRL-1504                                               

QY-x73      TE-1食管癌细胞                                                  

QY-x74      Bcap-37乳腺癌细胞                                                     

QY-x75      Eca-109食管癌细胞                                                     

QY-x76      MDA-MB-453乳腺癌细胞      HTB-131                                       

QY-x77      CNE鼻咽癌细胞                                                   

QY-x78      JAR胎盘绒毛癌细胞     HTB-144                                        【人低密度脂蛋白免疫复合物(LDL-IC)ELISA试剂盒厂家】

QY-x79      HEp-2喉表皮样癌细胞  CCL-23                                          

QY-x80      NIH:OVCAR-3卵巢癌细胞      HTB-161                                       

QY-x81      KB口腔表皮样癌细胞   CCL-17                                          

QY-x82      SK-OV-3卵巢癌细胞        HTB-77                                         

QY-x83      Acc-3涎腺腺样囊性癌细胞                                                        

QY-x84      HO-8910PM高转移卵巢癌细胞                                                  

QY-x85      Acc-2涎腺腺样囊性癌细胞                                                

QY-x86      HO-8910卵巢癌细胞                                                   

QY-x87      Tca-8113舌鳞癌细胞                                                   

QY-x88      HCC 94 [HCC941122]子宫鳞癌细胞高分化                                                 

QY-x89      A-431表皮癌细胞  CRL-1555                                               

QY-x90      HeLa 229宫颈癌细胞     CCL-2.1                                         

QY-x91      CBRH-7919肝癌细胞                                                   

9015970  Tube Holder, STS MDx, 9 mm (12)            CNY                             

9015636  Fuse-2, 3.15A (for PSB board), M48                   CNY                             

9015588  EZ1 DNA TISS Card  Pre-programmed card for BioRobot EZ1 DNA Tissue Protocol        CNY                             

9015586  Lid, Cover, PlateReader            CNY                             

9015540  Belt, Y                CNY                            

9019159  PCB 1 - Connection, PyroQ96MD              CNY                              【人低密度脂蛋白免疫复合物(LDL-IC)ELISA试剂盒厂家】

9014004  Light, Barrier, Drawer, BR8 RD                 CNY                             

9021165  QIAxcel ScreenGel Software (10 licenses)                 CNY                             

9021059  Door Assy, Upper Right, DCU            CNY                             

9021053  Controller, Rotary, DCU            CNY                             

9020940  Vacuum chamber (aluminum), QXT                   CNY                             

9020913  Slideout electrical tray, SMC, QXT            CNY                             

9020681  Screw, centre rail clamp, CX1           CNY                             

9020627  Door, consumables, left, Cabinet Qsym            CNY                             

9020483  Belt, timing, GSC               CNY                             

9020480  Sheet, therm conductive, 30 x 30, GSC             CNY                             

9020393  PCB, signal distr., chassis AS, Qsym           CNY                             

9020367  Worktable, body parts, AS, Qsym              CNY                             

9020366  Tip slide, AS, Qsym            CNY                             

9020362  Electronic box, AS, Qsym          CNY                             

9020334  PCB - Sensor w/thermal fuse, RGQII                  CNY                             

9020287  Adaptor, rotor disc 100, easylift, Cx2                CNY                              【人低密度脂蛋白免疫复合物(LDL-IC)ELISA试剂盒厂家】

9020263  Switch, assembly, lid, May03-Sep06, C1x                  CNY                             

9011906  Plate for accessories, complete                 CNY                             

9236991  Power Supply, 1000W, BR8 RD v1.0                  CNY                             

9019145  Fan - rear 60x60, PyroQ96MD                   CNY                             

9019119  Cog belt kit - X/Y, PyroQ96ID             CNY                             

9019112  PCB - Controller, PyroQ96ID              CNY                             

9019077  PyroMark Assay Design SW 2.0        All-in-one software for design of both PCR ａnd sequencing primers        CNY                            

9019074  PyroMark Storage Box 13x8x5cm, Pyro  Box for dust free ａnd safe storage of tips in-between runs in PyroMark Q96 MD.         CNY                             

9019013  Motor Z, EZ1xl                   CNY                             

9019011  Motor P, EZ1xl          CNY                             

9016060  Tubing Set, Waste Wash,BR8000              CNY                             

9019207  Cable - X  robot, PyroQ96MD           CNY                           

1066845  PG AIV-H5 RT-PCR Assay (48) LE Z          CNY                           

1066852  FMDV RT-PCR REAGENT LE            CNY                           

1060993  Buffer MW2 (87ml)              CNY                           

1054578  HiSpeed Plasmid Mega EF Kit (5)         CNY                           

1061091  HCV RT-PCR REAGENT VI        CNY                           

1061095  HSV PCR REAGENT II        CNY                            【人氧化低密度脂蛋白抗体(OLAb)ELISA试剂盒厂家】

1061100  HCMV Quantification Standards           CNY                           

1061102  TB PCR REAGENT I           CNY                           

1061106  MP Quantification Standards         CNY                           

9016732  Security Key, LC Policy Manager, encoded         CNY                           

9019887  Lid, label BC1, RG3              CNY                           

9019884  Cover, main, label CoResearch, RG3           CNY                           

9019883  Optical source ａnd detector assy , RG3              CNY                           

9019767  Sensor, interlock1, with cable, QX              CNY                           

9238801  Round Bucket w/ thread, sealable w/ cap     Sigma part no. 13350     CNY                           

9019725  Rotor-Disc Heat Sealer 230V        CNY                           

9019663  PyroMark Vac Prep Tool, PyroQ96VW        CNY                           

9019618  Motor, Z-axis, Cx2        CNY                           

9019615  Pipettor, spring, Cx2             CNY                           

9019600  O-ring, filter housing seal, 2mm, CX1         CNY                           

9019572  Pulley, Idler X-axis, Cxx              CNY                           

9019569  Stop pin, X-axis, Cxx           CNY                            【人氧化低密度脂蛋白抗体(OLAb)ELISA试剂盒厂家】

9019536  Pipetting Station RD, RCS            CNY                           

9019534  Home flag, Z-axis, CQx        CNY                           

9019531  UV light assembly, QIA         CNY                           

9019396  Motor, stepper, rotor drive, RG3          CNY                           

9019352  Solenoid, cooling flap, RG6          CNY                           

9019351  Fan, cooling blower, RG6             CNY                           

9019334  Kit, Heater FET & drivers, RG6           CNY                           

9234763  cable, flat band, 1500 mm            CNY                           

9234433  Boundary, SIS, rigth             CNY