检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供*优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【人肾上腺素能a1A受体(ADRA1A)ELISA试剂盒现货】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

【人肾上腺素能a1A受体(ADRA1A)ELISA试剂盒现货】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在、第二孔中分别加标准品100μl,然后在、第二孔中加标准品稀释液50μl,混匀；然后从孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品*终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

【人肾上腺素能a1A受体(ADRA1A)ELISA试剂盒现货】注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果.

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请*后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

【人肾上腺素能a1A受体(ADRA1A)ELISA试剂盒现货】计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

QYD585   禽流感ELISA试剂盒（AI-Ab)         96孔/盒                                      

QYD586   禽脑脊髓炎ELISA试剂盒（AE-Ab)        96孔/盒                                      

QYD587   传染性喉气管炎ELISA试剂盒（ILT-Ab)        96孔/盒                                      

QYD588   鸭肠炎沙门氏菌ELISA试剂盒（SE-Ab)        96孔/盒                                      

QYD589   鸭鼠伤寒沙门氏菌ELISA试剂盒 （ST-Ab）        96孔/盒                                      

QYD590   鸡传染性贫血ELISA试剂盒（CAV-Ab) 96孔/盒                                      

QYD591   伪狂犬 -抗体检测ELISA        96孔/盒                                      

QYD592   猪瘟 - 抗体检测ELISA 96孔/盒                                       【人肾上腺素能a1A受体(ADRA1A)ELISA试剂盒现货】

QYD593   猪瘟 - 抗原检测 ELISA        96孔/盒                                      

QYD594   口蹄疫-NS(3ABC) - 抗体检测ELISA       96孔/盒                                      

QYD595   口蹄疫-NS(3ABC)- 抗体检测ELISA        96孔/盒                                      

QYD596   口蹄疫 - 抗体检测ELISA      96孔/盒                                      

QYD597   蓝耳病 - 抗体检测ELISA      96孔/盒                                      

QYD598   圆环病毒 IgG - 抗体检测ELISA   96孔/盒                                      

QYD599   猪水泡病毒 - 抗体检测ELISA      96孔/盒                                      

QYD600   猪细小病毒 - 抗体检测ELISA: Ceditest PPV       96孔/盒                                      

QYD601   猪传染性胸膜肺炎 - 抗体检测ELISA：Ceditest APP 96孔/盒                                      

QYD602   猪瘟 - 抗体检测ELISA 96孔/盒                                      

QYD603   猪传染性胸膜肺炎 - 抗体检测ELISA 96孔/盒                                      

QYD604   猪丹毒 - 抗体检测ELISA      96孔/盒                                      

QYD605   猪丹毒 - 抗原检测ELISA      96孔/盒                                      

QYD606   猪传染性胃肠炎 - 抗体检测ELISA      96孔/盒                                      

QYD607   猪沙门氏菌 - 抗体检测ELISA      96孔/盒                                      

QYD608   猪流感- 抗体检测ELISA        96孔/盒                                       【人肾上腺素能a1A受体(ADRA1A)ELISA试剂盒现货】

QYD609   猪流感- 抗原检测ELISA        96孔/盒                                      

QYD610   猪副嗜血杆菌- 抗体检测ELISA    96孔/盒                                      

QYD611   猪轮状病毒- 抗体检测ELISA        96孔/盒                                      

QYD612   猪轮状病毒- 抗原检测ELISA        96孔/盒                                      

QYD613   反刍动物弓形虫抗体检测ELISA   96孔/盒                                      

QYD614   非洲猪瘟抗体检测试剂盒     96孔/盒                                      

QYD615   鲤鱼卵黄蛋白原试剂盒         96孔/盒                                      

9015514  PM Kit Biorobot M 96               CNY                             

800381     Database Search               CNY                             

133001     NeXtalStock 1,6-Hexanediol (200)            CNY                             

9243520  QIAlink, Special Integration Service         "QIAlink Special Integration Service includes:

On site software configuration for new instruments ａnd software configurationi for lab developed test. Service may be used also for customer training separately, a refresh training, or a training for new hires. All travel ａnd labor included."         CNY                             

9014406  Upgrade kit, LabHandT-RapidPlate          CNY                            

9014341  Tubing, pump inlet, DISU, BR8000           CNY                             

9019914  PCB, HEPA filter, CAS                 CNY                             

9018171  Light/uv, portal, Qsym               CNY                             

9018166  Ventilator, chassis, Qsym          CNY                             

9017852  Rotor Nut                   CNY                             

9017840  Tool, calibration tip, QIAC                  CNY                             

9017833  Sensor, door, QIAC            CNY                             

9017809  Foot, QIAC                 CNY                             

9017807  Drawer, waste, QIAC                 CNY                             

9017789  Motor, Y-drive, complete, QIAC                CNY                             

9017727  BracketContact, ProbeHolder, BR8 RD v4.0             CNY                              【人肾上腺素能a1A受体(ADRA1A)ELISA试剂盒现货】

9015283  Cover, Z, M48           CNY                             

9015274  Laboratory Cabinet BR8000RD                 CNY                             

9001909  QIAgility HEPA/UV (w/o PC) Priority         QIAgility HEPA/UV (w/o PC) Priority includes the QIAgility instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor ａnd travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents. CNY                             

133118     NeXtalStock tert-Butanol (200)                 CNY                             

133109     NeXtalStock Sodium chloride (200)           CNY                             

965652     QIAamp Virus BioRobot MDx Kit (12)      For 12 x 96 preps: 12 QIAamp 96 Plates,RNase-free Buffers,† QIAGEN Protease,Elution Microtubes CL, Caps, S-Blocks, Carrier RNA          CNY                             

965162     QIAamp DNA Blood BioRobot 9604 Kit (12)    For 12 x 96 DNA preps: 12 QIAamp 96 Plates, Buffers, QIAGEN Protease, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), Caps    CNY                             

962341     DirectPrep 96 BioRobot Kit (4)         For 4 x 96 plasmid minipreps: 4 DirectPrep 96 Plates, Reagents, Buffers, Flat-Bottom Blocks ａnd Lids, 96-Well Microplates FB, AirPore Tape Sheets, Tape Pads      CNY                             

9018535  Tool, door sensor deactivation, QIAC                CNY                             

133103     NeXtalStock Propylene glycol (200)          CNY                             

9015513  PM Kit Biorobot M 48               CNY                             

9015470  Motor, Z            CNY                             

9001887  QIAcube (230V), PrioPLUS        "QIAcube (230V) Instrument

Installation of QIAcube  instrument & Introductory training

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite PM per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

9001886  QIAcube (230V), Priority QIAcube (230V), Priority includes the QIAcube instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.      CNY                             

9018459  Safety valve, assy, QX                CNY                             

9018456  Linear guide, Y axis, QX             CNY                              【人肾上腺素能a1A受体(ADRA1A)ELISA试剂盒现货】

9012421  MP-Slot Extention             CNY