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齐一生物现货供应人ELISA试剂盒，大鼠ELISA试剂盒，小鼠ELISA试剂盒等现货充足，购买任意ELISA试剂盒都享受免费代测服务，更多进口原装品牌试剂盒供应齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人蛋白酶3特异性抗中性粒细胞胞质抗体(PR3-ANCA)ELISA试剂盒厂家】酶法检测试验所需自备物品:

1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

【人蛋白酶3特异性抗中性粒细胞胞质抗体(PR3-ANCA)ELISA试剂盒厂家】操作注意事项：

1.试剂盒应按标签说明书储存，使用前恢复到室温30分钟后方可使用。稀稀过后的标准品应丢弃，不可保存。

2.实验中不用的板条应立即放回包装袋中，密封保存，以免变质。

3.不用的其它试剂应包装好或盖好。不同批号的试剂不要混用.请在试剂盒保质前使用。

4.使用一次性的吸头以免交叉污染，吸取终止液和底物A、B液时，避免使用带金属部分的加样器。

5.使用干净的塑料容器配置洗涤液。使用前充分混匀试剂盒里的各种成份及样品。

6.洗涤酶标板时应充分拍干，不要将吸水纸直接放入酶标反应孔中吸水。

7.底物A应挥发，避免长时间打开盖子。底物B对光敏感，避免长时间暴露于光下。避免用手接触，有毒。实验完成后应立即读取OD值。

8.加入试剂的顺序应一致，以保证所有反应板孔温育的时间一样。

9.按照说明书中标明的时间、加液的量及顺序进行温育操作。

【人蛋白酶3特异性抗中性粒细胞胞质抗体(PR3-ANCA)ELISA试剂盒厂家】齐一生物客户寄标本时需注明以下情况：

1、标本编号；2、所测项目；3、是否做复孔；3、联系方式；4、实验后标本是否寄回。

客户必知：

客户应对所提供的材料及信息负责，如因客户提供的材料及信息不准确而引起的实验延误或经济损失由客户承担。

齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【人蛋白酶3特异性抗中性粒细胞胞质抗体(PR3-ANCA)ELISA试剂盒厂家】齐一生物供应，以上资料供参考如有疑问需要详细资料请联系我们客服人员，齐一生物销售：021－6034 8496；181214 53965；173021 04490

OPCM       少突胶质前体细胞培养基  Oligodendrocyte Precursor Cell Medium          500 ml                                 

OsM 球状少突细胞培养基  Oligosphere Medium        500 ml                                 

KM   角质细胞培养基  Keratinocyte Medium      500 ml                                 

KM-d         角质细胞培养基（确定）          Keratinocyte Medium-defined         500 ml                                 

FM-sf        成纤维细胞培养基         Fibroblast Medium-serum free        500 ml                                 

TEpiCM    扁桃体上皮细胞培养基         Tonsil Epithelial Cell Medium   500 ml                                 

OKM         口腔角质细胞培养基  Oral Keratinocyte Medium      500 ml                                  【人蛋白酶3特异性抗中性粒细胞胞质抗体(PR3-ANCA)ELISA试剂盒厂家】

CoEpiCM  结肠上皮细胞培养基     Colonic Epithelial Cell Medium         500 ml                                 

BEpiCM    支气管上皮细胞培养基       Bronchial Epithelial Cell Medium     500 ml                                 

SAEpiCM  气管上皮细胞培养基  Small Airway Epithelial Cell Medium        500 ml                                 

EpiCM-2   上皮细胞培养基-2        Epithelial Cell Medium-2 500 ml                                 

UCM         尿道上皮细胞培养基  Urothelial Cell Medium    500 ml                                 

PEpiCM    前列腺上皮细胞培养基       Prostate Epithelial Cell Medium       500 ml                                 

STEMium 人胚胎干细胞无血清培养基         Human Pluripotent Stem Cell Growth Medium       500 ml                                 

STEMium-XF     无异种人胚胎干细胞生长培养基         Xeno-free Human Pluripotent Stem Cell Growth Medium       500 ml                                 

MEF-cm   小鼠胚胎成纤维细胞培养基         Mouse Embryonic Fibroblasts-Conditioned Medium        100 ml                                

bFGF-std MEF-cm     碱性成纤维细胞生长因子刺激的小鼠胚胎成纤维细胞条件培养基         bFGF-Stimulated Mouse Embryonic Fibroblasts Conditioned Medium     100ml                                  

CEpiCM    角膜上皮细胞培养基  Corneal Epithelial Cell Medium        500 ml                                 

OEpiCM    卵巢上皮细胞培养基  Ovarian Epithelial Cell Medium        500 ml                                 

MSCM-sf  间充质干细胞培养基  Mesenchymal Stem Cell Medium-serum free 500 ml                                 

MEpiCM   乳腺上皮细胞培养基  Mammary Epithelial Cell Medium   500 ml                                 

KM-acf     角质细胞培养基  Keratinocyte Medium-animal component free        500 ml                                 

FM-acf      成纤维细胞培养基       Fibroblast Medium-animal component free    500 ml                                  【人蛋白酶3特异性抗中性粒细胞胞质抗体(PR3-ANCA)ELISA试剂盒厂家】

STEMium-ACF  人类多能干细胞培养基-无动物组分   STEMium® Human Pluripotent Stem Cell Growth Medium-animal component free          500ml                                  

MSCM-acf         间充质干细胞培养基     Mesenchymal Stem Cell Medium-animal component free        500 ml                            

MEM        MEM        MEM with Earle＇s Salts ａnd L-Glutamine        500 ml                                 

MEM       MEM       MEM with Hank＇s Salts ａnd L-Glutamine         500 ml                                 

MEM       MEM       MEM with Eagles＇s Salts ａnd Glutamine         500 ml                                 

M199E  M199E  Medium 199 with Earle＇s Salts ａnd L-Glutamine    500 ml                                 

M199H           M199H           Medium 199 with Hanks Salts ａnd L-Glutamine     500 ml                                 

DMEM    DMEM    DMEM with L-Glutamine ａnd Sodium Pyruvate     500 ml                                 

9021101  Skin Panel, Lower Left Rear, DCU             CNY                             

9238279  PAXgene 96 Incubator Block (farbl.elox)          CNY                              【人蛋白酶3特异性抗中性粒细胞胞质抗体(PR3-ANCA)ELISA试剂盒厂家】

9240677  Bumper, Round, Latch Lock, AP98            CNY                             

9240635  Filter, EMI,Modular, RJ45, AP196/98                 CNY                             

9240615  Fuse, 1.0A, FNM, AP96             CNY                             

9240612  Turk Sensor, Waste Bottle Level, AP                  CNY                             

9240603  Deck/Waste Overflow Sensor, AP196/98           CNY                             

9240583  Regulator, Pressure, Main, AP                   CNY                             

9240545  Hatch pad, Eppendorf, AP196/98               CNY                             

9240542  Lower Header, Capper Head, AP196                 CNY                             

9240521  Motor Assy, Shaker, AP98                  CNY                             

9240520  Motor Assy, Sz. 17, Common, AP98                   CNY                             

9240102  Re-Installation, LiquiChip          The Re-Installation, LiquiChip facilitates quick implementation of system back into lab routine by minimizing instrument set-up phase. It covers hardware ａnd software check of instrument ａnd provides user with operator refesher overview.  Re-installation includes labor ａnd travel costs. Re-installation performance process is documented on a GMP/GLP-compliant Field Service document.          CNY                             

9019940  Power supply, vac pump, CX1           CNY                             

9019477  LASER MODUAL GS          CNY                             

9019476  Spacer, 0.2 mm short set, GSC                   CNY                             

9238888  PCB, Main-01, BS96                   CNY                             

9238886  Cable, HM, BS96               CNY                              【人蛋白酶3特异性抗中性粒细胞胞质抗体(PR3-ANCA)ELISA试剂盒厂家】

9238854  #BR M96, Basic Agreement    The Basic Cover Agreement M96 provides full service coverage for the BioRobot M96. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                            

9238842  RS232 box, non-online fill, RapidPlate               CNY                             

9241215  Full Agreement Depot Repair, Shaker, 1yr       "The Full Agreement Depot Repair, Shaker provides full service coverage for the plate shaker used in the digene HPV application. The service is conducted as an instrument exchange service.

All shipping, labor ａnd repair part costs related to an instrument exchange are included for 1 year. Instrument exchange time is 2 - 3 working days."          CNY                             

9238833  #BR 9604, Basic Agreement    The Basic Cover Agreement 9604, 1 PM provides full service coverage for the BioRobot 9604. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9238832  BR 9600, Basic Agreement      The Basic Cover Agreement 9600 provides full service coverage for the BioRobot 9600. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                            

9238819  Rect. carrier for 5 glass tubes 25ml                   CNY                             

9237527  Software LiquiChip Workstation IS V2.2.          CNY                             

9237380  Installation, BR MDx & 2 react. setup     Installation ａnd training, including two customized reaction setup protocols         CNY                             

9237379  Installation, BR MDx & 1 react. setup     Installation ａnd training, including one customized reaction setup protocol CNY                             

9237378  BR MDx, Installation        The Installation, BR MDx facilitates the quick implementation of the BioRobot MDx into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the BioRobot MDx instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.         CNY                             

9237175  Guard Fan, Platic XYP                CNY                             

9233209  Tubing, 3/2,                CNY                             

9233155  Power Cord, European              CNY                             

9236418  Board, CPU, TWI               CNY                             

9236345  Red LSR             CNY                             

　　测定小分子量物质常用竞争法，其标准曲线中吸光度与受检物质的浓度呈负相关。标准曲线的形状因试剂盒所用模式的差别而略有不同。ELISA测定的标准曲线注意图中横坐标为对数关系，这更有利于测定系统的表达。

        讲述完了以上ELISA实验的操作要点，可以发现其实很多步骤都并不复杂，重要的是操作的时候一定要注重细节，熟能生巧，才可以掌握ELISA实验的精髓所在。

MaM        巨噬细胞的培养基         Macrophage Medium      500 ml                                 

MelM       黑色素细胞培养基       Melanocyte Medium        500 ml                                 

MelM-2    黑色素细胞培养基-2   Melanocyte Medium-2    500 ml                                 

FM   成纤维细胞培养基         Fibroblast Medium  500 ml                                 

FM-2         成纤维细胞培养基-2    Fibroblast Medium-2        500 ml                                 

AEpiCM    肺泡上皮细胞培养基     Alveolar Epithelial Cell Medium       500 ml                                  【人基质裂解素(ST3)ELISA试剂盒现货】

SkMCM    骨骼肌细胞培养基       Skeletal Muscle Cell Medium   500 ml                                 

EpiCM       上皮细胞培养基  Epithelial Cell Medium     500 ml                                 

EpiCM-a   动物上皮细胞培养基  Epithelial Cell Medium-animal          500 ml                                 

MCM        肾系膜细胞培养基       Mesangial Cell Medium   500 ml                                 

ObM         成骨细胞培养基  Osteoblast Medium 500 ml                                 

CM   软骨细胞培养基     Chondrocyte Medium      500 ml                                 

SM   滑膜细胞培养基  Synoviocyte Medium        500 ml                                 

NPCM       髓核细胞培养基  Nucleus Pulposus Cell Medium         500 ml                                 

HM  肝细胞培养基       Hepatocyte Medium        500 ml                                 

SteCM      星形细胞培养基  Stellate Cell Medium        500 ml                                 

CMM        心肌细胞培养基  Cardiac Myocyte Medium        500 ml                                 

TMCM      小梁网细胞培养基         "Trabecular Meshwork Cell Medium

"       500 ml                                 

TM   滋养层细胞培养基       Trophoblast Medium        500 ml                                 

AdM          脂肪细胞培养基     Adipocyte Medium  500ml                                  

PAM 前脂肪细胞培养基       Preadipocyte Medium     500 ml                                 

PADM       脂肪细胞诱导分化培养基     Preadipocyte Differentiation Medium     500 ml                                  【人基质裂解素(ST3)ELISA试剂盒现货】

MSCM      间充质干细胞培养基  Mesenchymal Stem Cell Medium    500 ml                                 

MODM-500      间充质干细胞-成骨细胞分化培养基 Mesenchymal Stem Cell Oseogenic Differentiation Medium  500 ml                                 

MODM-250               Mesenchymal Stem Cell Oseogenic Differentiation Medium  250 ml                                 

MADM-500      间充质干细胞-脂肪细胞分化培养基   Mesenchymal Stem Cell Adipogenic Differentiation Medium 500 ml                                 

MADM-250               Mesenchymal Stem Cell Adipogenic Differentiation Medium 250 ml                                 

MCDM     间充质干细胞-软骨分化培养基   Mesenchymal Stem Cell Chondrogenic Differentiation Medium     500ml                                  

SMCM-sf  平滑肌细胞培养基         Smooth Muscle Cell Medium- serum free        500 ml                                 

NPrCM     神经前体细胞培养基     Neural Precursor Cell Medium         500 ml                                 

NM  神经细胞培养基  Neuronal Medium   500 ml                                 

1061071  PG AIV-H5 RT-PCR Assay (48)  Z              CNY                             

1061075  PG NDV Univ. RT-PCR Assay (48) Z            CNY                             

1061085  TB PCR REAGENT II          CNY                              【人基质裂解素(ST3)ELISA试剂盒现货】

1061098  HIV-1 RT-PCR REAGENT II                  CNY                             

9016574  QIAamp Media MDx, PRO                 CNY                             

9016538  Transportation box , BioRobot EZ1           CNY                             

9016528  Transportation box , Access. Cabinet                CNY                             

9016471  BR MDx Service, PRO                CNY                             

9016150  #App. Package, M48, Forensics                 CNY                             

9016146  #App. Package, M48, Genotyping            CNY                             

9019182  Pump - Pressure, PyroQ96                 CNY                             

9019172  Contact pins - Heating block, PyroQ96              CNY                             

9011910  Tip-disposal unit, v2.03             CNY                             

9011893  Tubing, teflon, M6, 2.5 x 0.5 mm, 0.25 m                 CNY                             

9237081  Installation, BR plus 1 integration            CNY                             

9236921  Liquid Sensor, BR8 RD v1.0                CNY                             

9019148  IPC chassis, PyroQ96MD           CNY                             

9019076  PyroMark CDT Holder, PyroQ96MD                  CNY                             

9019006  Power supply, EZ1xl          CNY                              【人基质裂解素(ST3)ELISA试剂盒现货】

9018997  Cover, front, left, EZ1xl             CNY                             

9018957  Reagent Block, 17x5ml Flat -Base Tubes          CNY                             

9018936  Adapter, 16 tube-rack cepheid Smartcycle               CNY                             

9022748  Actuator, Tip Eject, DCU          CNY                             

9022733  RQ document, RGQ          CNY                             

9022708  Baffle, PMT, DML3000              CNY                             

9016065  Maintenance Kit, Basic, BR8000 I            CNY                             

9016063  Tubing Set, Waste DISU, BR8000              CNY                             

9015724  Probe, 0.9 mm, BR8000 v1.00                   CNY                             

9015534  Cover, front, type B          CNY                             

9015529  Filter, inlet, with switch & fuse                  CNY                             

9014202  Tubing Sys., Bottle, BR8 RD              CNY                             

9021194  Grid, fan, SP, Qsym            CNY                             

9021128  Cooling adapter, PCR 384, Qsym               CNY