齐一生物销售：021－6034 8496；181214 53965；173021 04490

1。我必须运行所有的标准和样品的重复？

是的，重复的运行，以监测测定的精度和提高检测结果的信心。

2。我必须在一次运行所有的样品吗？

不，每个试剂盒采用stripwell微孔板。这允许用户在不同的时间来分析不同数量的样品。

3。什么类型的可重复性的结果，得到与分析？

每一个工具都有一个包含一个典型数据的图表的手册。在运营商的任何变化，移液和洗涤技术，培养时间、温度、试剂盒的年龄可以引起变化的结果。每个用户都应该得到自己的标准曲线。

4。是否有可能储存的试剂比表示？

在不推荐使用条件下的部件组件的存储，以保证测试的正确性能。

5。我应该如何储存我的样品？

样品应保存在-20°C或更低的温度。长期储存，建议冻结他们在- 70余。

6。我可以修改协议吗？

BG的ELISA试剂盒进行了优化，提供*佳的结果。修改的格式或协议可能会给出错误的结果。

7。我可以使用一个不建议在试剂盒插入的样本类型吗？

该试剂盒已被验证在试剂盒插入的样品类型。未经检验的样品类型。为进一步信息的联系技术服务。

8。我的样本产生的值，在动态范围内的测定。我可以使用这些值吗？

建议只有在标准曲线的范围内下降的样本值。标准曲线的范围内的值一般是非线性的，这可能导致不正确的外推值。产生值高于标准的样品应为（进一步）稀释和重复。如果样品低于该测定的范围内，该样品被认为是不可检测的。

9。我每次都要运行一个空白或零标准吗？

是的，这些都是必需的计算，并反映任何微妙但显着的性能变化，从一天到一天，检测到检测。当排除特定的检测问题的来源时，它们也非常有帮助。

10。我能改变我在检测中使用的样本量吗？

不建议你改变卷由于BG工具被设计为*佳的性能在给定的体积

11。可以使用不同的组件组件吗？

每一个组件包含有特定批号的组件，以确保所有的组件都进行优化，以及与组件中的所有其他组件。对这些特定的地段进行质量检验。这是从来没有建议使用你自己的组件或组件从其他包或供应商。

12。我的标准曲线看起来很好，但我没有得到一个信号，我的样本，当我预期，为什么？

样品不包含被分析物。一个矩阵的效果可能是掩盖检测。确保推荐的稀释液在试剂盒插入后按规定进行。如果建议稀释，请检查是否正确进行稀释。过稀释会导致样品在标准曲线的范围内下降。

13。你怎么推荐我洗盘子？

如果您使用的是一个自动平板洗衣机，我们建议定期检查校准，并将该系统在洗涤前冲洗缓冲液冲洗。手动洗衣机也是一样的。一个中继器或洗瓶也可以使用。用户应小心，以确保所有的内容都是送气和板拍干的无尘纸。【人孕酮(PROG)ELISA现货】

14。我需要用一个盘子吗？

可靠的结果可以得到无板振动，但外径的一般会低于那些使用平板振动器。

15。为什么我要用波长校正450-570nm之间？

对于酶联免疫吸附试验，在双波长读的是做正确的光密度贡献的塑料以及，灯和光学波动。

16。如果我提我的样本，我还需要遵循推荐的稀释液试剂盒中插入了吗？

提取过程中所需的样本稀释量将受到影响的纯化和浓度的影响的协议使用。稀释或浓缩的金额将由*终用户决定。

17。什么是预期的分析物浓度，我应该找到？

一个给定的分析物的量可能会有所不同，不仅从物种的物种，但也组织和细胞来源。这个信息的*佳来源是目前的文献，很容易通过互联网在多个科学数据库访问。

18。我的光密度有点高（或较低），比那些在手册中，与我的试剂盒来了。为什么？

光学密度受影响

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【人孕酮(PROG)ELISA现货】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【人孕酮(PROG)ELISA现货】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【人孕酮(PROG)ELISA现货】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

 QY-04076          蛇毒巴曲酶抗体     Anti-Batroxobin                                           

 QY-04077          Bax（小鼠来源抗体）  Anti-Bax （Bcl-2 Assaciated X protein）                                           

 QY-04078          Bax抗体           Anti-Bax（Bcl-2 Assaciated X protein）                                              

 QY-04079          Bcl-2抗体        Anti-Bcl-2(B cell lymphoma/leukemia-2)                                             

 QY-04080          Bcl-xL蛋白抗体       Anti-Bcl-xL                                           

 QY-04081         p53正向细胞凋亡调控因子抗体         Anti-BBC3/PUMA(bcl-2 binding component 3)                                             

 QY-04082          抗磷酸化Bcl-xL(pSer62)蛋白抗体          Anti-phospho-Bcl-xL(pSer62)                                       

 QY-04083          丁酰胆碱脂酶抗体(N端)       Anti-BCHE(butyrylcholinesterase)NT                                           

 QY-04084          抗丁酰胆碱脂酶单克隆抗体         Anti-BCHE(mouse Anti-butyrylcholinesterase Monoclonal)                                        

 QY-04085          丁酰胆碱脂酶抗体(C端)        Anti-BCHE(butyrylcholinesterase)CT                                            【人孕酮(PROG)ELISA现货】

 QY-04086          脑源神经营养因子/脑衍化神经营养因子   Anti-BDNF (Brain-derived Neurotrophin factor)                                         

 QY-04087          一种抑癌基因抗体         Anti-BECN1(Beclin 1)                                           

 QY-04088          β-酪蛋白(抗体)兔抗山羊、绵羊          Anti-Beta-casein(β-casein)                                        

 QY-04089         TNF家族B细胞激活因子抗体    Anti-BAFF/CD257(B cell activating factor)                                          

 QY-04090          碱性成纤维细胞生长因子抗体     Anti-BFGF/FGF-2                                          

 QY-04091          骨保护蛋白/骨钙素抗体        Anti-BGP(Osteocalcin/Bone Gla-protein)                                            

 QY-04092          BH3结构域凋亡诱导蛋白抗体     Anti-Bid (BH3 interacting domain death agonist)                                       

 QY-04093         桥连整合因子蛋白抗体        Anti-BIN1(Bridging integrator protein-1)                                            

 QY-04094          细胞死亡调解子抗体     Anti-Bim(Bcl-2 interacting mediator of cell death)                                              

 QY-04095          细胞凋亡抑制因子5抗体     Anti-BIRC5/Survivin variant                                         

 QY-04096          BK通道蛋白抗体   Anti-BK channel (stretch-activated Kca channel)                                        

 QY-04097          钙激活钾通道蛋白抗体         Anti-BKCa channels（calcium-activated potassium channel）                                           

 QY-04098         骨形态发生蛋白-2抗体        Anti-BMP2 (bone morphogenetic protein 2)                                      

 QY-04099          骨形态发生蛋白4抗体          Anti-BMP4（bone morphogenetic protein 4）                                          

 QY-04100         骨形态发生蛋白-7抗体        Anti-BMP-7(bone morphogenetic protein-7)                                      

 QY-04101          骨成型蛋白受体1A抗体       Anti-BMPR-1A(Bone Morphogenetic protein Receptor-1A)                                        【人孕酮(PROG)ELISA现货】

 QY-04102          脑钠素/利钠肽抗体      Anti-BNP （Brain Natriuretic Peptide ）                                         

 QY-04103         B细胞特异性转录因子抗体         Anti-BOb-1(B-cell oct-binding protein 1)                                              

 QY-04104          B Raf抗体        Anti-B Raf                                            

 QY-04105          乳腺癌易感基因1抗体(人)  Anti-BRCA1（Breast cancer susceptbility gene 1）(human)                                     

 QY-04106          乳腺癌易感基因1抗体（小鼠 大鼠）       Anti-BRCA1（Breast cancer susceptbility gene 1）（mouse rat）                                             

292015     mericon Pig Kit (96)          CNY                             

133123     NeXtalStock Tris (200)               CNY                             

965672     QIAamp One-For-All Nucleic Acid Kit (12)                  CNY                             

133192     NeXtalStock Ammonium tartrate pH 7 (200)           CNY                             

133183     NeXtalStock Citric acid pH 3.5 (200)                  CNY                             

9018513  Adapter, USB-serial, EZ1a                 CNY                             

9001896  QIAxtractor, UV/HEPA (w/o PC) System  QIAxtractor, UV/HEPA (w/o PC) System combines the QIAxtractor system ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with a training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document. CNY                             

51104       QIAamp DNA Blood Mini Kit (50)     For 50 DNA minipreps: 50 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2 ml)        CNY                             

133099     NeXtalStock Potassium nitrate (200)                 CNY                             

133092     NeXtalStock PEG 8,000 (200)            CNY                             

9015516  Heat unit, complete                  CNY                             

9018452  Piston, purge (1), QX                  CNY                             

9012416  Microplate Station 8000, short        Station for accommodating a 96-well plate (or other labware with the same base dimensions) on the BioRobot 8000 or MDx worktable    CNY                             

133168     NeXtalStock CAPSO pH 9.2 (200)               CNY                             

9018424  Door, N2, QX             CNY                              【人孕酮(PROG)ELISA现货】

9015405  Board, PDB, M96              CNY                             

9015386  Manual, Service, BIOROBOT MDx            CNY                             

9001863  Rotor-Gene Q 2plex HRM (PC) Priority    Rotor-Gene Q 2plex HRM (PC) Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.        CNY                             

9001720  Rotor-Gene Q 6Plex no PC                 CNY                             

9012331  Set, Tubing, Pipetting, Plato3xx1              CNY                             

133084     NeXtalStock PEG 300 (200)               CNY                             

38065       #PNA Cartridge (5ml)                CNY                             

204752     QuantiFast Multiplex PCR +R Kit (80)                CNY                             

290095     mericon Quant. L. pneumophila Kit (96)          CNY                             

800423     Library, Large Insert, Picked Clones                   CNY                             

290045     mericon Campylobacter triple Kit (96)              CNY                             

9015353  Controller, temp.               CNY                             

133051     NeXtalStock HEPES sod. salt untit. (200)          CNY                             

9018388  QIAcube Tip Adapter Ring Tool                 CNY                             

9018377  Teaching Tools, Alignment, AP                   CNY                             

9012226  Power Supply, 6 amp for Plato size 90               CNY                              【人孕酮(PROG)ELISA现货】

9012066  Tray, buffer bottles, roter, BR8000           CNY                             

9001674  PyroMark Q96 MD Automated Instrument     For performing automated pyrosequncing reaction on the Q96 MD      CNY