1.购买我公司任意ELISA试剂盒指标享受免费代测，并提供技术指导服务！

二、实验材料与试剂配制：

1. 仪器与材料：酶标仪（使用前预热30分钟），微量加液器、吸头、蒸馏水或去离子水，滤纸。

2. 缓冲液使用：加5ul  的缓冲液于50ul  的样品中，如果样品量不够或者不确定，缓冲液和样品的混合比例不要小于1:10即可混匀，静置1小时备用（如果标本是血清或者血浆，此步骤忽略）。

3. 洗液的配制：按1:100的比例配制洗液备用。

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人非典型性肺炎(SARS-Ab)elisa厂家】三. 仔细阅读说明书

1.检查试剂盒标签上的有效期。如果超过有效期，请勿使用。

2. 按说明书确定所有试剂齐全（数量、体积）

3. 标本制备要规范，每份标本体积要按2-3个复孔以上的量制备（贮存），尽量分装做备份。

4.短期内无法实验者，注意低温保存

5.准备好所有实验额外所需物品，（比如移液器，试管，清洗器，酶标仪）

6. 按说明书将所用试剂平衡至室温，根据检测标本数量确定所需试剂的量

7.检查试剂盒内每种试剂的贮存条件，保证所有试剂均按照试剂盒内说明书推荐的条件存储。 【人非典型性肺炎(SARS-Ab)elisa厂家】

8.检查不稳定或者变质的试剂溶液（比如沉淀或变色）。有些试剂，比如标准品稀释液或者检测稀释液，可能在设计时就含有沉淀物。所有试剂在滴入酶标板之前，必需充分混匀。而由于沉淀物的特性，在加入酶标板之前，必需不停搅拌。

9.保证充分的孵育时间和温度。

10.切勿使用不同生产批号的试剂取代现有试剂或混合现有试剂。

11.在混合或溶解蛋白溶液时，避免泡沫产生。

12.在实验开始之前，安排好实验流程。在实验开始之前，清洁工作台。

13.若有问题，应与我公司或代理商的技术支持联系

【人非典型性肺炎(SARS-Ab)elisa厂家】四、客户须知

齐一生物销售：021－6034 8496；181214 53965；173021 04490

1.在收集标本前都必须有一个完整的计划，必须清楚要检测的成份是否足够稳定。我们提倡新鲜标本尽早检测，对收集后当天就进行检测的标本，及时储存在4℃备用，如有特殊原因需要周期收集标本，请造模取材后，将标本及时分装后放在-20℃或-70℃条件下保存。因冰室与室温存在一定温差，蛋白极易降解，直接影响实验质量，所以避免反复冻融。部分标本也可加入适当防腐剂，激素类标本需添加抑肽酶。 

2.试剂盒代测标本的客户取材前须向我司销售人员索要说明书，具体操作注意事项请与我司技术人员沟通。

3.液体类标本：标本必须为液体，不含沉淀。包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清、组织匀浆等。

4.购买前请提前告诉我们：您样本的种属、样本数量、待测指标及其它特殊要求。

【人非典型性肺炎(SARS-Ab)elisa厂家】五、代测数据详细内容

1、ELISA标准曲线；

2、实验具体详细步骤；

3、完整的实验报告（试剂耗材，实验方法，实验结果及结果说明）；

4、实验进行阶段，我公司客服人员会及时向您汇报实验进程。

 QY-04107          乳腺癌易感基因2抗体          Anti-BRCA2（Breast cancer susceptbility gene 2）                                           

 QY-04108          溴脱氧尿苷抗体     Anti-BrdU (Bromodeoxyuridine)                                          

 QY-04109         溴脱氧尿苷单克隆抗体        Anti-BrdU (Bromodeoxyuridine)                                          

 QY-04110          溴脱氧尿苷抗体     Anti-BrdU (Bromodeoxyuridine)                                          

QY-0001   RCA (bovine serum albumin) 牛血清白蛋白            5g/8010g/140100g/1200                                    

QY-0002   KLH (keyhole limpet hemocyanin) 血蓝蛋白           10mg/24050mg/1100100mg/1580                            

QY-0003   OVA (ovalbumin) 鸡卵白蛋白                   10mg/10050mg/300100mg/500                                 

QY-0004   THY (thyroglobulin,TG) 甲状腺球蛋白            10mg/15050mg/650                                   

QY-0005   rHSA人血清白蛋白                   10mg/80100mg/480                                   

QY-00060 Recomb Protein G-HRP酶标重组蛋白G                  0.1ml/0                               

QY-04370 Streptavidin,SA链霉亲和素             1mg/2205mg/88010mg/1600                             【人非典型性肺炎(SARS-Ab)elisa厂家】

QY－－0007  Recomb Protein G/FITC荧光素（FITC）标记重组蛋白-G              0.5ml/850                                   

QY-03620 Recomb Protein A重组蛋白A                   1mg/2005mg/80020mg/2500                            

QY-03610 Recomb Protein G重组蛋白G                  1mg/2505mg/1500                            

QY-8280   Cholesterol胆固醇            100g/260500g/10801Kg/1580                                    

QY-01040 β-Amyloid（1-16）β淀粉样肽1-16（多肽蛋白）              0.5mg/880                                  

QY-08710 Aβ31-35（β-Amyloid 31-35）β-淀粉样肽31-35                 0.1mg/3200.2mg/480                                 

QY-01050 β-Amyloid（1-28）β淀粉样肽1-28（多肽蛋白）              0.5mg/880                                  

QY-01070 β-Amyloid fragment（1-42）Ratβ淀粉样肽（1-42）大鼠                 0.1mg/6000.5mg/2200                              

QY-01060 β-Amyloid（1-40)β淀粉样肽1-40（多肽蛋白）                 0.1mg/5000.5mg/20001mg/3100                              

QY-01070 β-Amyloid fragment（1-42）β淀粉样肽（1-42）（抗原）                 0.1mg/6000.5mg/2200                              

QY-00440 β-Amyloid 25-35 Aβ 25-35β-淀粉样肽25-35             1mg/4005mg/150050mg/15000                                

QY-00760 β-Amyloid 1-42 CTβ淀粉样肽 1-42 (C端多肽)             0.1mg/5000.5mg/20001mg/3100                              

QY-00570 Amylin糖尿病相关肽（胰岛淀粉样肽）               0.5mg/6501mg/1100                                  

QY-01120 APP695-770/APP17肽 （Amyloid protein precursor）淀粉样肽前体蛋白（17肽抗原）                   0.5mg/6501mg/1100                                    

QY-04990 Neuroprotective peptide/Gly-HN(Nuclear-encoded Humanin CAS:HN(N) Gly14)神经保护肽                  0.5mg/1100                                

QY-0158   Amylin(1-37)糖尿病相关肽（胰岛淀粉样肽）               0.1mg/6500.5mg/2480                               【人非典型性肺炎(SARS-Ab)elisa厂家】

QY-10530 CCP peptide (cyclic citrullinated peptide,CCP)瓜氨酸环肽                0.5mg/11001mg/1800                               

QY-06160 CCP peptide (cyclic citrullinated peptide,CCP)瓜氨酸环肽                0.5mg/1100                               

QY-00380 GLP-1（Glucagon-like peptide-1）(7-36)胰高血糖素样肽-1（多肽）                    0.1mg/5000.5mg/2000                              

QY-0108   GRGDS peptide抗黏附多肽GRGDS                 1mg/3005mg/1200                            

9001658  Rotor-Gene Q 5plex HRM (w/o PC) Priority     Rotor-Gene Q 5plex HRM (w/o PC) Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.        CNY                             

951054     EZ1 DNA Blood 350 µl Kit (48)          "

48 Reagent Cartridges (Blood 350 ul), 50 Disposable Tip Holders, 50 Disposable Filter-Tips, 50 Sample Tubes (2.0 ml), 50 Elution Tubes (1.5 ml)"  CNY                             

9001874  EZ1 Advanced XL, System         EZ1 Advanced XL, System combines the EZ1 Advanced XL instrument ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.          CNY                             

9015466  Cover, P             CNY                             

9012413  Z-Bars Gripper, RCS1                 CNY                             

9012410  Tischplatte Plato 3301S Technikk  (9194)                CNY                             

205110     Omniscript RT Kit (10)      For 10 reverse-transcription reactions: 40 units Omniscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free water         CNY                             

204845     QuantiTect Multiplex RT-PCR NR Kit(1000)               CNY                             

133178     NeXtalStock Citric acid pH 2.6 (200)                  CNY                             

133165     NeXtalStock CAPS pH 11.4 (200)               CNY                             

9018409  Regulator, N2, internal, front, QX            CNY                             

9015406  Driver, motor, M96           CNY                             

9001861  Rotor-Gene Q 2plex (PC) Priority     Rotor-Gene Q 2plex (PC) Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.        CNY                             

9012327  Peristaltic Pump Outlet Tubing                  CNY                              【人非典型性肺炎(SARS-Ab)elisa厂家】

1067925  Buffer AW1 (175 ml)                  CNY                             

38045       GNA Cartridge (5ml)                  CNY                             

38003       ConA Cartridges (3 x 1ml)                  CNY                             

9015365  Drop catcher, M96           CNY                             

204741     QuantiTect Multiplex PCR NoROX Kit (40)       "Trial kit size for 40 x 50 ul reactions: 1.7 ml QuantiTect Multiplex PCR NoROX Master Mix, 2.0 ml RNase-free water

For quantitative, real-time PCR ａnd two-step RT-PCR using probe detection with multiple assays."     CNY                             

133066     NeXtalStock Manganese(II) chloride (200)                CNY                             

290065     mericon Cronobacter spp Kit (96)             CNY                             

204374     Rotor-Gene Probe PCR Kit (400)      The Rotor-Gene Probe PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly sensitive quantification in real-time PCR ａnd two-step RT-PCR using sequence-specific probes.  CNY                             

9015354  Springs, gas (2)                   CNY                             

133054     NeXtalStock Imidazole HCl (200)               CNY                             

9001671  PyroMark Q24 Instrument       For methylation ａnd mutation analysis using Pyrosequencing technology in 24 well      CNY                             

950901     Capture Plate, standard yield (5)              CNY                             

37430       PhosphoSerine Antibody Q5     100 ug mixture of anti-phosphoserine antibodies(isotypes mouse IgG1 ａnd IgM, for 200 ml working solution)     CNY                             

133030     NeXtalStock Cesium sulfate (200)             CNY                             

9001627  Rotor-Gene Q 2plex (w/o PC) System       Rotor-Gene Q 2plex (w/o PC) System Priority includes the Rotor-Gene system ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It covers the handling of the Rotorgene-Q systems hardware ａnd trains the user on operation of the Rotorgene software. All travel ａnd labor cost included for Installation ａnd service documented on a GMP/GLP-compliant Field Service document.  CNY                             

9001620  Rotor-Gene Q 2plex System              CNY                              【人非典型性肺炎(SARS-Ab)elisa厂家】

9001603  Rotor-Disc 100 Starter Kit (230V)             CNY                             

9015316  Belt, Y-motor, v2, M48              CNY                             

9015312  Unit, spotlight, right, M48                CNY