1.购买我公司任意ELISA试剂盒指标享受免费代测，并提供技术指导服务！

二、实验材料与试剂配制：

1. 仪器与材料：酶标仪（使用前预热30分钟），微量加液器、吸头、蒸馏水或去离子水，滤纸。

2. 缓冲液使用：加5ul  的缓冲液于50ul  的样品中，如果样品量不够或者不确定，缓冲液和样品的混合比例不要小于1:10即可混匀，静置1小时备用（如果标本是血清或者血浆，此步骤忽略）。

3. 洗液的配制：按1:100的比例配制洗液备用。

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人硫酸肝素糖蛋白(HSPG)ELISA试剂盒厂家】三. 仔细阅读说明书

1.检查试剂盒标签上的有效期。如果超过有效期，请勿使用。

2. 按说明书确定所有试剂齐全（数量、体积）

3. 标本制备要规范，每份标本体积要按2-3个复孔以上的量制备（贮存），尽量分装做备份。

4.短期内无法实验者，注意低温保存

5.准备好所有实验额外所需物品，（比如移液器，试管，清洗器，酶标仪）

6. 按说明书将所用试剂平衡至室温，根据检测标本数量确定所需试剂的量

7.检查试剂盒内每种试剂的贮存条件，保证所有试剂均按照试剂盒内说明书推荐的条件存储。 【人硫酸肝素糖蛋白(HSPG)ELISA试剂盒厂家】

8.检查不稳定或者变质的试剂溶液（比如沉淀或变色）。有些试剂，比如标准品稀释液或者检测稀释液，可能在设计时就含有沉淀物。所有试剂在滴入酶标板之前，必需充分混匀。而由于沉淀物的特性，在加入酶标板之前，必需不停搅拌。

9.保证充分的孵育时间和温度。

10.切勿使用不同生产批号的试剂取代现有试剂或混合现有试剂。

11.在混合或溶解蛋白溶液时，避免泡沫产生。

12.在实验开始之前，安排好实验流程。在实验开始之前，清洁工作台。

13.若有问题，应与我公司或代理商的技术支持联系

【人硫酸肝素糖蛋白(HSPG)ELISA试剂盒厂家】四、客户须知

齐一生物销售：021－6034 8496；181214 53965；173021 04490

1.在收集标本前都必须有一个完整的计划，必须清楚要检测的成份是否足够稳定。我们提倡新鲜标本尽早检测，对收集后当天就进行检测的标本，及时储存在4℃备用，如有特殊原因需要周期收集标本，请造模取材后，将标本及时分装后放在-20℃或-70℃条件下保存。因冰室与室温存在一定温差，蛋白极易降解，直接影响实验质量，所以避免反复冻融。部分标本也可加入适当防腐剂，激素类标本需添加抑肽酶。 

2.试剂盒代测标本的客户取材前须向我司销售人员索要说明书，具体操作注意事项请与我司技术人员沟通。

3.液体类标本：标本必须为液体，不含沉淀。包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清、组织匀浆等。

4.购买前请提前告诉我们：您样本的种属、样本数量、待测指标及其它特殊要求。

【人硫酸肝素糖蛋白(HSPG)ELISA试剂盒厂家】五、代测数据详细内容

1、ELISA标准曲线；

2、实验具体详细步骤；

3、完整的实验报告（试剂耗材，实验方法，实验结果及结果说明）；

4、实验进行阶段，我公司客服人员会及时向您汇报实验进程。

9020319  PM kit, C12 & QIAgility (UV), CG1             CNY                             

9017625  Motor, y-drive (24V), BR8RD            CNY                             

9020254  LED, LightStrip (white), Qsym           CNY                             

9017354  Holder, VacTrap, BR8                CNY                             

9020201  PCB, lrge breakout, 12 core to rev8, CC2                  CNY                             

9020199  PCB, Z plate breakout,8 ch., 8 core, QX1                  CNY                             

9020193  PCB, vac pump, QXT                  CNY                             

9239523  Fitting,1/16MNPT,1/8, forWasteBottle(5)                  CNY                             

9239536  Assy,Terminated,RedLED,SDS (2)              CNY                             

9239529  Fuse&Holder,1Amp,Fblo,PicoSmd(5)                 CNY                             

9020159  Mask, protection - chamber bottom, RG3                CNY                             

9020145  QIAplex MDD HPV Genotyping LQ Software            CNY                             

9020114  USB cable, QGX                 CNY                             

9020102  Packaging, TLyser2           CNY                              【人硫酸肝素糖蛋白(HSPG)ELISA试剂盒厂家】

9020100  Shipping Lock Screw, TLyser 2           CNY                             

9020094  Left Ratchet Holder, TLyser 2           CNY                             

9020090  Motor 24V DC, TLyser 2            CNY                             

9239518  Tubing. Peek, 1/6OD, 010ID, 10 (qty 5)             CNY                             

9239492  Hyd Arm Optic Switch               CNY                             

9239449  3A, 250V Fast Acting Fuse                 CNY                             

9232867  Sensor, pressure, 1600-1mbar                   CNY                             

9236775  Sealing ring, auto vac, SBS top, BR3000           CNY                             

9236509  BR 8000, PM Service        The Overhaul covers all travel ａnd labor costs plus all necessary parts to perform a Preventative Maintenance,. The instrument will also be thoroughly checked ａnd inspected to determine if any additional parts are required to get the instrument running to its optimal performance. There will be an additional charge for any such parts if any are needed.     CNY                             

9239360  Z-Axis Coupling, EZ1                   CNY                             

9236478  BR 9604, Full Agreement         The Full Agreement 9604, 1 PM provides full service coverage for the BioRobot 9604. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).         CNY                             

9236476  #Agreement, 9604, 2PM, 1 yr        An agreement for preventive maintenance provides coverage for travel, labor, ａnd parts, plus the cost of consumables required for two maintenance visits during 1 year. Work carried out during the preventive maintenance visit is fully documented in accordance with GMP/GLP directives. CNY                             

9232726  Block, 2-way, v1.02           CNY                             

9239540  Cable Assy Extender, 2 wire, 22AWG, 12in                CNY                             

9239396  Upgr. LiquiChip Analyzer (RUO)                 CNY                             

9239496  EX XY Motor Driver w/Encoder                 CNY                              【人硫酸肝素糖蛋白(HSPG)ELISA试剂盒厂家】

9239494  Sheath Fluid Tubing w/DeBubbler             CNY                             

QY-x638   PC-3前列腺癌细胞                                                       

QY-x639   BEL-7402肝细胞癌细胞                                                       

QY-x640   Tsu-Pr1非雄激素依赖型前列腺癌                                                      

QY-x641   HCC-9204肝癌细胞                                                      

QY-x642   HOS骨肉瘤细胞                                                   

QY-x643   Hep3B肝癌细胞                                                   

QY-x644   MG-63成骨肉瘤细胞                                                  

QY-x645   Hep G2肝癌细胞                                                 

QY-x646   Saos-2骨肉瘤细胞                                               

QY-x647   SMMC-7721肝癌细胞                                                   【人硫酸肝素糖蛋白(HSPG)ELISA试剂盒厂家】

QY-x648   U-2 OS骨肉瘤细胞                                                       

QY-x649   PAN-1胰腺导管上皮癌细胞                                               

QY-x650   OS-732骨肉瘤细胞（瘤株）                                                       

QY-x651   Panc 10.05胰腺腺癌细胞                                                    

QY-x652   A-204横纹肌肉瘤细胞                                                

QY-x653   SW1990胰腺腺癌细胞                                                

QY-x654   HT1080纤维肉瘤细胞                                                 

QY-x655   SK-RC-42肾癌细胞                                               

QY-x656   A375皮肤黑色素瘤细胞                                                      

QY-x657   RCC-krause肾癌细胞                                                    

QY-x658   A875黑色素瘤细胞                                                      

QY-x659   Ketr-3肾癌（瘤株）                                                    

QY-x660   SK-HEL-1皮肤黑色素瘤细胞                                               

QY-x661   SW 13肾上腺皮质瘤细胞                                                    

QY-x662   A431皮肤基底细胞癌细胞                                                 

QY-x663   96-C低转移肺癌细胞                                                   【人硫酸肝素糖蛋白(HSPG)ELISA试剂盒厂家】

QY-x664   CEM白血病细胞                                                  

QY-x665   95-D高转移肺癌细胞                                                  

QY-x666   K562慢性髓原白血病细胞                                                 

QY-x667   973肺腺癌细胞                                                    

QY-x668   HPB-ALLT细胞白血病细胞                                                  

QY-x669   A2肺腺癌细胞                                                      

QY-x670   HL-60白血病细胞