价格详细资料：试剂盒价格电议，齐一生物销售：021－6034 8496；181214 53965；173021 04490,齐一生物科技（上海）有限公司提供ELISA试剂盒受到了广大研究所.中科院科研单位一致肯定和认同。齐一生物大品牌保证，倾力为国内外科研院校实验室提供*优质产品。

【人血小板反应蛋白(TSP-1)ELISA试剂盒厂家】注意事项

1. 当混合蛋白溶液时应尽量轻缓，避免起泡。

2. 洗涤过程非常重要，不充分的洗涤易造成假阳性。

3. 一次加样时间控制在5分钟内，如标本数量多，推荐使用排枪加样。

4. 请每次测定的同时做标准曲线，做复孔。

5. 如标本中待测物质含量过高，请先稀释后再测定，计算时请*后乘以稀释倍数。

6. 在配制标准品、检测溶液工作液时，请以相应的稀释液配制，不能混淆。

7. 底物请避光保存。

8. 不要用其它生产厂家的试剂替换试剂盒中的试剂。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【人血小板反应蛋白(TSP-1)ELISA试剂盒厂家】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【人血小板反应蛋白(TSP-1)ELISA试剂盒厂家】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【人血小板反应蛋白(TSP-1)ELISA试剂盒厂家】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

9013170       Tubing, syringe inlet 2, BR8000         CNY                          

9011417       Klemmplättchen Varispan P7 (Alodine)           CNY                          

5050-1020EN     #Keyboard English Spare          CNY                          

9240731       Tubing, 3/8"OD , Blue, AP         CNY                          

676091  ipsogen CBFB-MYH11 A Standards              CNY                          

9011341       Microplate Washer Module Plato 3          CNY                          

9011203       Vacuum control unit, BR8000           CNY                          

673522  ipsogen JAK2 MutaQuant Kit (12) CE           CNY                          

337839  qBiomarker SYBR Fluor Mastermix 25 ml             CNY                          

337632  qBiomarker SYBR Green ROX Mastermix (12)           CNY                          

9018907       36-Well Rotor             CNY                           【人血小板反应蛋白(TSP-1)ELISA试剂盒厂家】

9018783       CAT, Qsym          CNY                          

9018694       EZ1 Adv. XL DNA Bacteria Card           CNY                          

9018565       Door lock, mechanical, EZ1a            CNY                          

9013132       Elevating Platform        CNY                          

4509063       artus WNV LC RT-PCR Kit (24) CE             CNY                          

9001905       #QIAgility (w/o PC) System      QIAgility (w/o PC) System combines the QIAgility instrument ａnd installation. The Installation plus Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.   CNY                          

800383  Open Reading Frame Search            CNY                          

9019052       Notebook 2 XP          CNY                          

9020200       ZLV PCB, USB, 18Q        CNY                          

9020580       Cable, DB9 M/F (UL) 6ft, DML 3000           CNY                          

9020588       Pulley, belt, DML 3000              CNY                          

9021016       Robot Stage, X Axis, VR, DCU              CNY                          

9013376       Maintenance Kit BR 8000 Research        CNY                          

9013279       BGR Upgrade-Kit Scanner PK80           CNY                          

9013272       Peristaltic Pump, RCS1             CNY                          

9013190       Motor, rotor, buffer track, BR8000          CNY                           【人血小板反应蛋白(TSP-1)ELISA试剂盒厂家】

132772  NeXtal Stock Jeffamine M-600 (200)      100% v/v O-(2-Aminopropyl)-O＇-(2-methoxyethyl)polypropylene 500 - 200mL (20 x 10 ml tube)    CNY                          

132620  NeXtal Stock Tricine (50)   1M Tricine - 50mL (4 x 12,5 ml tube)      CNY                          

132560  NeXtal Stock Magnesium chloride (50)   2M Magnesium chloride hexahydrate - 50mL (4 x 12,5 ml tube)  CNY                           

800171  Easy-Read Tubes, w/o prep       "Sequencing of plasmid DNA ａnd PCR products

DNA quality control not included

No repetition of sequencing

Data collected to maximal read length"     CNY                          

QY-x2086     HIH 3T3 小鼠胚胎成纤维细胞 1640+10%胎牛血清                                      

QY-x2087     L929 小鼠成纤维细胞 1640+10%胎牛血清                                     

QY-x2088     HSC-T6 大鼠肝星状细胞 1640+10%胎牛血清                                       

QY-x2089     L-02 人肝细胞 1640+10%胎牛血清                                    

QY-x2090     SRA01/04 人晶体上皮细胞 MEM+非必需氨基酸＋10%胎牛血清                                   

QY-x2091     CHO 中国仓鼠卵巢细胞（野生型） 1640+10%胎牛血清                                         

QY-x2092     IEC-6 大鼠小肠隐窝上皮细胞 DMEM+10%胎牛血清                                         

QY-x2093     P815 小鼠肥大细胞 1640+10%胎牛血清                                         

QY-x2094     NRK-49F 大鼠正常肾成纤维细胞 1640+10%胎牛血清                                      

QY-x2095     自建 正常成人皮肤成纤维细胞 DMEM+20%胎牛血清 原代培养                                   

QY-x2096     HELF 人胚肺成纤维细胞 1640+10%胎牛血清 5，6代                                      

QY-x2097     293 人胚肾细胞 DMEM+10%胎牛血清                                     

QY-x2098     293T 人胚肾T细胞 1640+10%胎牛血清                                          【人血小板反应蛋白(TSP-1)ELISA试剂盒厂家】

QY-x2099     GES-1 人胃粘膜细胞 DMEM+10%胎牛血清                                   

QY-x2100     3T3-L1 小鼠胚胎成纤维细胞（前脂肪） DMEM+10%胎牛血清                                     

QY-x2101     Vero 猴肾细胞 1640+10%胎牛血清                                    

QY-x2102     CHO-dhFr- 中国仓鼠卵巢细胞(二氢叶酸缺陷型） 1640+10%胎牛血清                                       

QY-x2103     V79 中国仓鼠肺细胞 1640+10%胎牛血清                                       

QY-x2104     PA317 小鼠胚胎成纤维细胞 1640+10%胎牛血清                                         

QY-x2105     Cos-7 绿猴肾细胞 DMEM+10%胎牛血清                                        

QY-x2106     MA104 非洲绿猴胚胎肾上皮细胞 DMEM+10%胎牛血清                                         

QY-x2107     16HBE 人支气管上皮细胞 1640+10%胎牛血清                                     

QY-x2108     Marc145 猴胚胎肾上皮细胞                                   

QY-x2109     MDA-MB-435S 人乳腺导管癌细胞                                     

QY-x2110     MDBK 瘤牛肾细胞                                   

QY-x2111     MEG-01 人成巨核细胞白血病细胞                                     

QY-x2112     MFC 小鼠胃癌细胞                                   

QY-x2113     MG-63 人骨肉瘤细胞                                      

QY-x2114     MLA144长臂猿淋巴瘤细胞                                          【人血小板反应蛋白(TSP-1)ELISA试剂盒厂家】

QY-x2115     MouseM 小鼠肌肉细胞                                    

QY-x2116     MouseS 小鼠皮肤成纤维样细胞                                   

QY-x2117     Hep G2人肝癌细胞系                                      

QY-x2118     Hep3B人肝癌细胞系