【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【人糖化蛋白(GSP)ELISA试剂盒现货】样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【人糖化蛋白(GSP)ELISA试剂盒现货】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

【人糖化蛋白(GSP)ELISA试剂盒现货】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【人糖化蛋白(GSP)ELISA试剂盒现货】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

293035    mericon Apricot kernels (96)        CNY                           

929520    QX Alignment Marker 15bp/500bp (1.5 ml)         CNY                           

9012643  Bottle, system liquid              CNY                            【人糖化蛋白(GSP)ELISA试剂盒现货】

9012596  Disposable Tips, 300µl (960) Conducting disposable tips    CNY                           

133121    NeXtalStock Lithium citrate  (200)            CNY                           

133119    NeXtalStock Ammonium citrate  (200)              CNY                           

133190    NeXtalStock D-(+)-Glucose (200)              CNY                           

51162      QIAamp 96 DNA Blood Kit (12)    For 12 x 96 DNA preps: 12 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels  CNY                           

50112      QIAamp UCP PurePathogen Blood Kit (10)         CNY                           

133104    NeXtalStock Sodium acet.  (200)              CNY                           

9015468  Sensor, photo, PM-L24         CNY                           

9001871  EZ1 Advanced, System  EZ1 Advanced, System combines the EZ1 Advanced system ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with a training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.    CNY                           

9018458  Screw, w finishing washer (4), QX             CNY                           

9018447  Purge Tool, cartridge, QX            CNY                           

9012412  Incubator Tower (65°) for Plato 3 hold        CNY                           

9012392  Bottle, waste, 10 l, BR8000          CNY                           

205111    Omniscript RT Kit (50)  For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free water      CNY                           

9018425  Door, sample, QX         CNY                           

9002123  QIAxcel Advanced System    QIAxcel Advanced System combines the QIAxcel Advanced instrument ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.    CNY                            【人糖化蛋白(GSP)ELISA试剂盒现货】

57714     QIAamp MinElute Virus Vacuum Kit (50)     For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, Carrier RNA, Buffers, Extension Tubes (3 ml), Collection Tubes (1.5 ml)     CNY                           

972012    PyroMark Q96 CpG p16 (96)       For quantification of methylation level in p16       CNY                           

133227    NeXtalStock Sodium cacodyl. pH 6.0 (100)        CNY                           

301108    PolyFect Transfection Reagent (100 ml)      For 2500-6500 transfections in 60 mm dishes or 5000-10,000 transfections in 6-well plates.  CNY                           

56304     QIAamp DNA Micro Kit (50) For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Carrier RNA, Buffers, Collection Tubes (2 ml)  CNY                           

9002031  Rotor-Gene Q MDx 5Plex HRM no PC        CNY                           

9002018  Rotor-Gene Q MDx 2plex HRM, Priority     Rotor-Gene Q 2plex HRM, Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents. CNY                           

970902    PyroMark Q24 Advanced Kit (4x24)            CNY                           

133211    NeXtalStock Magnesium chloride H2O (200)             CNY                           

133195    NeXtalStock Sodium tartrate pH 5.6 (200)          CNY                           

293023    mericon Corn (24)         CNY                           

292055    mericon Goat Kit (96)           CNY                           

EK0544   hVEGFR-ELISA     人VEGFR2/KDRELISA试剂盒   Human VEGFR2/KDR ELISA Kit  96 tests                

EK0561   hECad-ELISA 人钙粘附蛋白ELISA试剂盒       Human E-Cadherin ELISA Kit       96 tests                

EK0563   hCT1-ELISA  人心营养素-1ELISA试剂盒 Human Cardiotrophin-1 ELISA Kit 96 tests                

EK0573   hCD40L-ELISA     人可溶性CD40LELISA试剂盒   Human soluble CD40L ELISA Kit  96 tests                

EK0575   hEGVEGF-ELISA  人内分泌腺来源的血管内皮生长因子ELISA试剂盒       Human EG-VEGF ELISA Kit 96 tests                

EK0581   hRes-ELISA   人抵抗素ELISAELISA试剂盒    Human Resistin ELISA Kit     96 tests                 【人糖化蛋白(GSP)ELISA试剂盒现货】

EK0584   hTNF      人TNFβELISA试剂盒       Human TNFβ ELISA Kit     96 tests                

EK0588   hVEGFC-ELISA     人VEGF-CELISA试剂盒     Human VEGF-C ELISA Kit    96 tests                

EK0641   hSurv-ELISA  人生存素ELISA试剂盒       Human Survivin ELISA Kit    96 tests                

EK0642   hTSP2-ELISA 人TSP2ELISA试剂盒  Human TSP2 ELISA Kit 96 tests                

EK0644   hCD105-ELISA      人CD105ELISA试剂盒       Human CD105 ELISA Kit      96 tests                

EK0658   hAPP-ELISA  人APP ELISA试剂盒   Human APP ELISA Kit   96 tests                

EK0663   hBAFF-ELISA       人 BAFF ELISA试剂盒       Human BAFF ELISA Kit 96 tests                

EK0667   hPCad-ELISA 人P-CadherinELISA试剂盒 Human P-Cadherin ELISA Kit       96 tests                

EK0702   hCD40-ELISA       人CD40ELISA试剂盒  Human CD40 ELISA Kit 96 tests                

EK0706   hNCAM-ELISA      人CD56/NCAM-1ELISA试剂盒  Human CD56/NCAM-1 ELISA Kit 96 tests                

EK0744   hcMet-ELISA 人c-Met/HGFRELISA试剂盒      Human c-Met/HGFR ELISA Kit    96 tests                

EK0756   hErbB2-ELISA       人ErbB-2/Neu2ELISA试剂盒      Human ErbB-2/Neu2 ELISA Kit    96 tests                

EK0757   hFeuA-ELISA "人胎球蛋白AELISA试剂盒

"      HumanFetuin-A ELISA Kit     96 tests                

EK0770   hHBEGF-ELISA     人肝素结合表皮生长因子ELISA试剂盒   Human HBEGF ELISA Kit     96 tests                

EK0777   hIL6Ra-ELISA       人IL-6RaELISA试剂盒      Human IL-6Ra ELISA Kit      96 tests                

EK0779   hIL7-ELISA    人IL-7ELISA试剂盒   Human IL-7 ELISA Kit   96 tests                 【人糖化蛋白(GSP)ELISA试剂盒现货】

EK0799   hIL27-ELISA  人IL-27ELISA试剂盒        Human IL-27 ELISA Kit 96 tests                

EK0807   hCSF1R-ELISA     人CSF1R/M-CSFRELISA试剂盒      Human CSF1R/M-CSFR ELISA Kit      96 tests                

EK0816   hKal3-ELISA  人Kallikrein 3ELISA试剂盒 Human Kallikrein 3 ELISA Kit       96 tests                

EK0829   hRANK-ELISA      人 Receptor Activator of NF-κB (RANK)ELISA试剂盒   Human Receptor Activator of NF-κB (RANK) ELISA Kit 96 tests                

EK0850   hMPO-ELISA 人过氧化物酶ELISA试剂盒       Human Myeloperoxidase (MPO) ELISA Kit  96 tests                

EK0859   hPAI1-ELISA  人PAI-1ELISA试剂盒  Human PAI-1 ELISA Kit 96 tests                

EK0867   hDKK1-ELISA       人DKK-1ELISA试剂盒       Human DKK-1 ELISA Kit      96 tests                

EK0873   hPECAM-ELISA    人PECAM-1/CD31ELISA试剂盒       Human PECAM-1/CD31 ELISA Kit      96 tests                

EK0884   hBMP7-ELISA       人BMP-7ELISA试剂盒       Human BMP-7 ELISA Kit      96 tests                

EK0895   hp53-ELISA   人p53 ELISA试剂盒    Human p53 ELISA Kit    96 tests                

EK0904   hCEA-ELISA  人CEAELISA试剂盒    Human CEA ELISA Kit   96 tests