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人免疫抑制酸性蛋白(IAP)ELISA试剂盒现货
点击次数:16发布时间:2016/8/17 16:22:36
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更新日期:2016/8/17 16:22:36
所 在 地:中国大陆
产品型号:
优质供应
详细内容
【友情提示】:本产品仅供科研研究使用,不得用于人体临床直接检测。避免给您带来不必要的损失,请仔细阅读购买说明!
本试剂盒仅供研究使用,齐一生物销售:021-6034 8496;181214 53965;173021 04490欢迎选购!
操作步骤
1.准备试剂,样品和标准品
2.加入准备好的样品和标准品,37℃反应30分钟
3.洗板5次,加入酶标试剂,37℃反应30分钟
4.洗板5次,加入显色液A、B,37℃显色15分钟
5.加入终止液
6.15分钟之内读OD值
7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性,在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。
【人免疫抑制酸性蛋白(IAP)ELISA试剂盒现货】样本保存条件:
1.2-8℃冰箱*多放2周
2.-20到-50℃的冰箱可以放3-6个月
3.-50℃以下的冰箱,可以放一年
【人免疫抑制酸性蛋白(IAP)ELISA试剂盒现货】样本收集
1.血清样本用干燥管(红色)或者促凝管(黄色)收集
2.血浆用抗凝管,一般有EDTA抗凝(紫色)、枸橼酸钠抗凝(黑色或者蓝色)
3.动物组织匀浆(细胞):
1g组织加入9ml PBS(浓度为0.1M,pH为7.4的)测分泌型指标
1g组织加入1ml组织裂解液加入8ml PBS 测细胞内
粪便样本:1g样本+9ml PBS(浓度为0.01M,pH为7.4的)
没有PBS的话也可以用一下生理盐水,还是用PBS
【人免疫抑制酸性蛋白(IAP)ELISA试剂盒现货】样本离心转速及时间
1.试管采集的样本:3000转,离心五分钟取上清
2.离心管或者EP管采集的样本:5000--8000转3分钟取上清
3..样本保存--如果样品不立即使用,应将其分成小部分-70 ℃保存,避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒,检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。
【人免疫抑制酸性蛋白(IAP)ELISA试剂盒现货】以上样本采集及处理方式供您参考,如有问题请来电,齐一生物销售:021-6034 8496;181214 53965;173021 04490
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