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【人支气管上皮细胞 HBE】齐一生物提供多国品牌细胞产品,公司常年备用细胞现货,能满足全国客户对细胞研究的需求。齐一生物销售:021-6034 8496;181214 53965;173021 04490
选择齐一生物的原因:
1.供货及时。我们订货周期短,运输时间短,在*短时间内为您提供所需产品。
2.价格优惠:美国ATCC的中国代理商,没有中间环节。在国内我们保证价格。
3.服务保障:我们拥有专用的技术指导团队,对每售出的一件产品,提供全面的售前、售中和售后服务。
4.产品种类齐全:25个系统共150多种细胞以及各类菌株,满足不同客户的要求。细胞经过严格的质控,纯度可达98%。常用产品备有现货。其产品类型如下:消化系统细胞株,呼吸系统细胞株,泌尿系统细胞株生殖系统细胞株 血液循环系统 内皮细胞细胞株 神经系统及其他细胞株,动物细胞株和菌株
5.对于部分难培养的细胞我公司可代复苏细胞,并提供技术支持。
6.经验丰富:我公司是国内专业化的细胞销售公司,具有十余年细胞产品销售经验,有专业的销售团队和技术人员。齐一生物公司的每一位员工都将竭诚为您服务。
7.质量保证,保证售后
8. 齐一生物销售:021-6034 8496;181214 53965;173021 04490
【人支气管上皮细胞 HBE】 齐一生物公司专业代理ScienCell简介ScienCell Research Laboratories (ScienCell) is a biotechnology company whose mission is the research and development of cell and cell-related products for experimental and therapeutic use. The ScienCell technology provides a variety of high quality normal human and animal cells, cell culture media and reagents, medium supplements, cell-derived RNA, cDNA and proteins for the research community. Scientists at ScienCell are also studying and developing cell therapeutic strategies that will significantly improve the quality of life by achieving the cell's ability to combat human degenerative disorders. We are based in San Diego, California and were established in 1999.
【人支气管上皮细胞 HBE】美国ATCC简介:美国菌种保藏中心, 又称美国模式菌种收集中心(ATCC),是位于马里兰洲洛克菲勒的一家私营的,非赢利性组织。目前它可以提供各种动植物细胞、标准品、菌株达两万多种。ATCC成立于1925年,是世界上的生物资源中心,由美国14家生化、医学类行业协会组成的理事会负责管理,是一家全球性、非盈利生物标准品资源中心。ATCC向全球发布其获取、鉴定、保存及开发的生物标准品,推动科学研究的验证、应用及进步 ATCC可以提供以下类别生物标准品:细胞株(3000多种);菌株(15000多种);动植物病毒株(2500多种)以及重组物质等。
现已成为可信赖的活体微生物、细胞系等获得、保存和发放的国家资源中心。ATCC有29,000多种不同品系可靠的动物细胞和微生物培养体,它能满足各科学团体对可信赖品系的需要。仅1981年就有39,000多培养物发放给全世界的科学工作者,并成功地用冷冻和冷冻干燥的方法保存各种细胞品系。 ATCC是一个非(学会单位)无利润的公司,由各科学组织推选成员组成理事会进行管理。现有工作人员125人。参加的科学团体有:美国免疫协会、美国生物化学会、美国细胞生物学会、美国微生物学会、美国动物学会、美国遗传学会、原生动物学会和组织培养协会等16个学术组织。在ATCC可利用各种材料以及工具和设备进行广泛的研究。主要研究项目有:酶联免疫吸附分析技术(ELISA),真核细胞转录的调控,杂交瘤、淋巴类和髓类以及其他细胞系的建立,人突变细胞系的基因蛋白质,借助计算机进行鉴别分析,培养物的特征鉴别和保存技术的改进,关于化学致癌、致畸和致突变的。
【人支气管上皮细胞 HBE】齐一生物提醒接收到细胞注意事项
1. 接收到细胞,肉眼观察细胞培养基颜色,显微镜观察细胞生长情况,并对细胞进行不同倍数拍照(建议收到时的培养瓶拍一张照片,显微镜拍收到时的细胞100X,200X各一张)。
2.用75%的酒精消毒(建议配置75%的酒精的水是灭菌过的)。
3.严格无菌操作,打开细胞培养瓶。
4.将细胞培养瓶内的培养基用吸管完全吸出(严禁直接倾倒),放入另一无菌容器中(建议换新的培养基培养细胞)。
5.用PBS 2-3ml/次轻轻洗细胞表面,洗3-4次遍,加入0.05%胰酶-EDTA1-1.5ml,显微镜下观察细胞变圆,轻轻拍打培养瓶直到细胞脱落,加入终止液终止。
6.1000rpm,5min离心,重悬细胞。
7.换新的细胞培养瓶,37℃,5%CO2培养。【人支气管上皮细胞 HBE】
齐一生物销售:021-6034 8496;181214 53965;173021 04490
9013224Gripper set, V-handler, BR3000CNY
9013197Tubing, L1 outlet, auto vac,BR8000 v1.00CNY
9011702Cable, PCON98 to AC-box (level sensor),CNY
9011681Dispenser drive, BR8000CNY
132635NeXtal Stock Zinc chloride (50)0,1M Zinc chloride - 50mL (4 x 12,5 ml tube)CNY
132578NeXtal Stock PEG 2,000 DME (50)50% w/v Polyethylene glycol 2,000 dimethyl ether - 50mL (4 x 12,5 ml tube)CNY
800341Cosmid End-Sequencing, w/ prepCNY
800308siRNA Hairpin Run w/ prepCNY
800121Verification Sequencing, w/ prep"Double-stranded sequencing of a given sequence
QIAGEN® DNA template preparation
Design and synthesis of walking primers
Data assembly and sequence alignment
Accuracy >99.99%"CNY
132528NeXtal Stock CAPSO untit. (50)0.5M CAPSO - Untitrated - 50mL (4 x 12,5 ml tube)CNY
132510NeXtal Stock ADA untit. (50)0.5M ADA - Untitrated - 50mL (4 x 12,5 ml tube)CNY
764114PAXgene Bone Marrow RNA Tubes (50)CNY
679913ipsogen RT Kit (33)CNY
203643HotStarTaq Plus Master Mix Kit (250)"For 250 x 20 µl reactions: 3 x 0.85 ml HotStarTaq Plus Master Mix, containing 250 units of HotStarTaq Plus DNA Polymerase total, 1 x 0.55 ml CoralLoad Concentrate, 2 x 1.9 ml RNase-free Water
HotStarTaq Plus Master Mix Kits offer all the benefits of great specificity, sensitivity, and performance as HotStarTaq Plus DNA Polymerase NOW in a master mix format, which offers even faster results and added convenience."CNY
201225Taq PCR Core Kit (1000)"4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2, dNTP Mix†
NEW! Now with 10x CoralLoad PCR Buffer
Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity and minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye and a red dye, that facilitate estimation of DNA migration distance and optimization of agarose gel run time."CNY
201203Taq DNA Polymerase (250)"250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2
NEW! Now with 10x CoralLoad PCR Buffer
Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity and minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye and a red dye, that facilitate estimation of DNA migration distance and optimization of agarose gel run time."CNY
180922GeneRead Adapter L Set 1plex (12)CNY
180912GeneRead Adapter I Set 1plex (12)CNY
159214QIAcard FTA Indicator Four Spots (25)CNY
159201QIAcard FTA One Spot (100)CNY
9235523Needle cleaning assembly P/N 4567-01CNY
9235300Plunger tip, syringe 2,5mlCNY
9235117Fuse F4A, v1.00CNY
9022818EZ1 Advanced XL Flip Cap Tube RackCNY
9234889Vacuum manifold, 96 sealCNY
9234862System liquid container (2l Nalgene)CNY
9239555PCBA, Laser Driver, UnlimitedCNY
9238664HingeCNY
9238662Lid with windowCNY
9238659Cooling compressor T2168GK, 60HzCNY