1.购买我公司任意ELISA试剂盒指标享受免费代测，并提供技术指导服务！

二、实验材料与试剂配制：

1. 仪器与材料：酶标仪（使用前预热30分钟），微量加液器、吸头、蒸馏水或去离子水，滤纸。

2. 缓冲液使用：加5ul  的缓冲液于50ul  的样品中，如果样品量不够或者不确定，缓冲液和样品的混合比例不要小于1:10即可混匀，静置1小时备用（如果标本是血清或者血浆，此步骤忽略）。

3. 洗液的配制：按1:100的比例配制洗液备用。

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【小鼠Toll样受体9(TLR-9/CD289)ELISA试剂盒厂家】三. 仔细阅读说明书

1.检查试剂盒标签上的有效期。如果超过有效期，请勿使用。

2. 按说明书确定所有试剂齐全（数量、体积）

3. 标本制备要规范，每份标本体积要按2-3个复孔以上的量制备（贮存），尽量分装做备份。

4.短期内无法实验者，注意低温保存

5.准备好所有实验额外所需物品，（比如移液器，试管，清洗器，酶标仪）

6. 按说明书将所用试剂平衡至室温，根据检测标本数量确定所需试剂的量

7.检查试剂盒内每种试剂的贮存条件，保证所有试剂均按照试剂盒内说明书推荐的条件存储。 【小鼠Toll样受体9(TLR-9/CD289)ELISA试剂盒厂家】

8.检查不稳定或者变质的试剂溶液（比如沉淀或变色）。有些试剂，比如标准品稀释液或者检测稀释液，可能在设计时就含有沉淀物。所有试剂在滴入酶标板之前，必需充分混匀。而由于沉淀物的特性，在加入酶标板之前，必需不停搅拌。

9.保证充分的孵育时间和温度。

10.切勿使用不同生产批号的试剂取代现有试剂或混合现有试剂。

11.在混合或溶解蛋白溶液时，避免泡沫产生。

12.在实验开始之前，安排好实验流程。在实验开始之前，清洁工作台。

13.若有问题，应与我公司或代理商的技术支持联系

【小鼠Toll样受体9(TLR-9/CD289)ELISA试剂盒厂家】四、客户须知

齐一生物销售：021－6034 8496；181214 53965；173021 04490

1.在收集标本前都必须有一个完整的计划，必须清楚要检测的成份是否足够稳定。我们提倡新鲜标本尽早检测，对收集后当天就进行检测的标本，及时储存在4℃备用，如有特殊原因需要周期收集标本，请造模取材后，将标本及时分装后放在-20℃或-70℃条件下保存。因冰室与室温存在一定温差，蛋白极易降解，直接影响实验质量，所以避免反复冻融。部分标本也可加入适当防腐剂，激素类标本需添加抑肽酶。 

2.试剂盒代测标本的客户取材前须向我司销售人员索要说明书，具体操作注意事项请与我司技术人员沟通。

3.液体类标本：标本必须为液体，不含沉淀。包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清、组织匀浆等。

4.购买前请提前告诉我们：您样本的种属、样本数量、待测指标及其它特殊要求。

【小鼠Toll样受体9(TLR-9/CD289)ELISA试剂盒厂家】五、代测数据详细内容

1、ELISA标准曲线；

2、实验具体详细步骤；

3、完整的实验报告（试剂耗材，实验方法，实验结果及结果说明）；

4、实验进行阶段，我公司客服人员会及时向您汇报实验进程。

9239498  V-Housing, LX100              CNY                              【小鼠Toll样受体9(TLR-9/CD289)ELISA试剂盒厂家】

9239490  EX Laser Driver, Uniphase                  CNY                             

9239390  PCR Plate Adapter, M96           CNY                             

9236510  BR 3000, PM Service        The Overhaul covers all travel ａnd labor costs plus all necessary parts to perform a Preventative Maintenance,. The instrument will also be thoroughly checked ａnd inspected to determine if any additional parts are required to get the instrument running to its optimal performance. There will be an additional charge for any such parts if any are needed.     CNY                             

9232764  Disposable Troughs, 20 / 30 ml (10)          Troughs holding up to 20 ml of liquid; pack of 10     CNY                             

9236472  BR 9600, Full Agreement         The Full Agreement 9600 provides full service coverage for the BioRobot 9600. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).  CNY                             

9236443  BR, Installation         The Installation, BR Universal facilitates the quick implementation of the BioRobot into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the BioRobot Universal instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.         CNY                             

9239155  Assembly, vertical axis, TWI              CNY                             

9239108  Assy, Sample Needle, Actuator linkage             CNY                             

9232693  MTP-Plate Holder  2.01                   CNY                             

9232629  Bottle, 500-ml, yellow fitting            CNY                             

9232602  Vacuum manifold, plate holder bottomless              CNY                             

9232579  Cooling block, 1,5ml                   CNY                             

9238951  Locking lever, BS15           CNY                             

9238798  Rectangular bucket, sealable with cap             CNY                             

9238794  Angle rotor 6 x 250 ml               CNY                             

9016913  Tubing, press relief valve inlet DISU v2             CNY                              【小鼠Toll样受体9(TLR-9/CD289)ELISA试剂盒厂家】

9238942  Keyboard-Display assembly, BS15             CNY                             

9238753  Screwdriver, hex2mm, z-drive BR8          CNY                             

9238916  Power supply 40W, BS96          CNY                             

9238748  Coupling, CPC, screw, male, V2                 CNY                             

9238716  Seal for lid, 1350 mm                 CNY                             

9238898  Front panel, BS15              CNY                             

9238699  Frontpanel w. keyboard foil (Zent1)                   CNY                             

9231793  Motor, gripper, handler, BR8000              CNY                             

9238690  Computer board Zent2, no Eprom            CNY                             

9231434  Probe, 0,4mm, ceramic coated for PK80                   CNY                             

9020071  Keypad, TLyser                   CNY                             

9020070  Controller 100-120V/200-240V, TLyser              CNY                             

9014996  Shaker, Highspeed Unit, BR8 RD               CNY                             

9014870  X-Motor, BR8            CNY                             

9014790  Tip Disposal Bags MDx    Autoclavable bags for collection ａnd disposal of used disposable tips, pack of 15   CNY                            

QR20-110         Rosetta(DE3)感受态细胞        10 x 100μl                                           

QR20-111         Rosetta(DE3)感受态细胞        20 x 100μl                                           

QR20-112         BCA蛋白定量试剂盒     200次                                           【小鼠Toll样受体9(TLR-9/CD289)ELISA试剂盒厂家】

QR20-113         蛋白标准溶液-BSA 5x1ml                                            

QR20-114         SDS-PAGE凝胶制备试剂盒    1 kit                                      

QR20-115         ProteinShow-G250蛋白快速染色试剂 250ml                                           

QR20-116         30%丙烯酰胺-甲叉双丙烯酰胺溶液(29:1)   100ml                                           

QR20-117         SDS-PAGE浓缩胶缓冲液(4x)  50ml                                              

QR20-118         SDS-PAGE浓缩胶缓冲液(4x)  200ml                                           

QR20-119         SDS-PAGE分离胶缓冲液(4x)  100ml                                           

QR20-120         SDS-PAGE分离胶缓冲液(4x)  500ml                                           

QR20-121         SDS-PAGE Loading Buffer (变性，5x)      5ml                                       

QR20-122         SDS-PAGE Loading Buffer (非变性，5x) 5ml                                       

QR20-123         10%SDS溶液   100ml                                           

QR20-124         PBS  500ml                                           

QR20-125         PBST         500ml                                           

QR20-126         TBS  500ml                                           

QR20-127         TBST         500ml                                           

QR20-128         Tris-Glycine       500ml                                            【小鼠Toll样受体9(TLR-9/CD289)ELISA试剂盒厂家】

QR20-129         Tris-Glycine SDS        500ml                                           

QR20-130         1.5M Tris-Cl(pH8.8) 500ml                                           

QR20-131         0.5M Tris-Cl(pH6.8) 500ml                                           

QR20-132         超纯dNTP(2.5mM each)         0.5ml                                            

QR20-133         超纯dNTP(2.5mM each)         1ml                                       

QR20-134         超纯dNTP(10mM each）       250μl                                          

QR20-135         超纯dNTP(10mM each）       500μl                                          

QR20-136         超纯dATP(100mM)          0.5ml                                            

QR20-137         超纯dGTP(100mM)         0.5ml                                            

QR20-138         超纯dCTP(100mM)          0.5ml                                            

QR20-139         超纯dTTP(100mM)          0.5ml