齐一生物销售：021－6034 8496；181214 53965；173021 04490

1。我必须运行所有的标准和样品的重复？

是的，重复的运行，以监测测定的精度和提高检测结果的信心。

2。我必须在一次运行所有的样品吗？

不，每个试剂盒采用stripwell微孔板。这允许用户在不同的时间来分析不同数量的样品。

3。什么类型的可重复性的结果，得到与分析？

每一个工具都有一个包含一个典型数据的图表的手册。在运营商的任何变化，移液和洗涤技术，培养时间、温度、试剂盒的年龄可以引起变化的结果。每个用户都应该得到自己的标准曲线。

4。是否有可能储存的试剂比表示？

在不推荐使用条件下的部件组件的存储，以保证测试的正确性能。

5。我应该如何储存我的样品？

样品应保存在-20°C或更低的温度。长期储存，建议冻结他们在- 70余。

6。我可以修改协议吗？

BG的ELISA试剂盒进行了优化，提供*佳的结果。修改的格式或协议可能会给出错误的结果。

7。我可以使用一个不建议在试剂盒插入的样本类型吗？

该试剂盒已被验证在试剂盒插入的样品类型。未经检验的样品类型。为进一步信息的联系技术服务。

8。我的样本产生的值，在动态范围内的测定。我可以使用这些值吗？

建议只有在标准曲线的范围内下降的样本值。标准曲线的范围内的值一般是非线性的，这可能导致不正确的外推值。产生值高于标准的样品应为（进一步）稀释和重复。如果样品低于该测定的范围内，该样品被认为是不可检测的。

9。我每次都要运行一个空白或零标准吗？

是的，这些都是必需的计算，并反映任何微妙但显着的性能变化，从一天到一天，检测到检测。当排除特定的检测问题的来源时，它们也非常有帮助。

10。我能改变我在检测中使用的样本量吗？

不建议你改变卷由于BG工具被设计为*佳的性能在给定的体积

11。可以使用不同的组件组件吗？

每一个组件包含有特定批号的组件，以确保所有的组件都进行优化，以及与组件中的所有其他组件。对这些特定的地段进行质量检验。这是从来没有建议使用你自己的组件或组件从其他包或供应商。

12。我的标准曲线看起来很好，但我没有得到一个信号，我的样本，当我预期，为什么？

样品不包含被分析物。一个矩阵的效果可能是掩盖检测。确保推荐的稀释液在试剂盒插入后按规定进行。如果建议稀释，请检查是否正确进行稀释。过稀释会导致样品在标准曲线的范围内下降。

13。你怎么推荐我洗盘子？

如果您使用的是一个自动平板洗衣机，我们建议定期检查校准，并将该系统在洗涤前冲洗缓冲液冲洗。手动洗衣机也是一样的。一个中继器或洗瓶也可以使用。用户应小心，以确保所有的内容都是送气和板拍干的无尘纸。【大鼠组蛋白H2b(histon-H2b)ELISA试剂盒现货】

14。我需要用一个盘子吗？

可靠的结果可以得到无板振动，但外径的一般会低于那些使用平板振动器。

15。为什么我要用波长校正450-570nm之间？

对于酶联免疫吸附试验，在双波长读的是做正确的光密度贡献的塑料以及，灯和光学波动。

16。如果我提我的样本，我还需要遵循推荐的稀释液试剂盒中插入了吗？

提取过程中所需的样本稀释量将受到影响的纯化和浓度的影响的协议使用。稀释或浓缩的金额将由*终用户决定。

17。什么是预期的分析物浓度，我应该找到？

一个给定的分析物的量可能会有所不同，不仅从物种的物种，但也组织和细胞来源。这个信息的*佳来源是目前的文献，很容易通过互联网在多个科学数据库访问。

18。我的光密度有点高（或较低），比那些在手册中，与我的试剂盒来了。为什么？

光学密度受影响

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【大鼠组蛋白H2b(histon-H2b)ELISA试剂盒现货】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【大鼠组蛋白H2b(histon-H2b)ELISA试剂盒现货】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【大鼠组蛋白H2b(histon-H2b)ELISA试剂盒现货】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

301707     HiPerFect Transfection Reagent (4x1 ml)                  CNY                              【大鼠组蛋白H2b(histon-H2b)ELISA试剂盒现货】

59760       EpiTect Fast 96 LyseAll Bisulfite Kit           CNY                             

206624     Type-it CNV SYBR Green PCR Core Kit(400)              CNY                             

9012739  Peristaltic Pump outlet tubing 1.25                   CNY                             

133242     NeXtalStock Sodium malonate pH 4.0 (200)             CNY                             

133235     NeXtalStock Sodium citrate pH 5.0 (200)                  CNY                             

59496       EpiTect MethyLight PCR +ROX Kit (200)           CNY                             

59305       EpiTect MSP PCR Kit (100)                 CNY                             

59144       EpiTect Plus FFPE Bisulfite Kit (48)            CNY                             

929562     QX Size Marker 250 bp - 4 kb v2.0(50 µl)                   CNY                             

929550     QX DNA Size Marker pUC18/HaeIII (50 µl)                CNY                             

929529     QX Alignment Marker 50 bp/5 kb (1.5 ml)                 CNY                             

972824     PyroMark Gold Q96 CDT Reagents (6 x 96)     For performing pyrosequencing reaction on the Q96 MD in combination with the capillary dispensing tips (CDT)   CNY                             

133222     NeXtalStock Sodium ac. pH 4.0 (200)                CNY                             

133219     NeXtalStock Sodium ac. H2O (200)           CNY                             

52926       QIAamp Viral RNA Mini QIAcube Kit (240)               CNY                             

9012692  Source rack (128 pos) for tubes 16 mm             CNY                              【大鼠组蛋白H2b(histon-H2b)ELISA试剂盒现货】

9012665  Pipetting system upgrade 1, BR3000                CNY                             

9002022  Rotor-Gene Q MDx 5plex Platform          CNY                             

970012     PyroMark Q24 CpG p16 (4 x 24)       For quantification of methylation level in  region +148 to +174 in exon 1 of the gene.         CNY                             

133203     NeXtalStock Imidazole-HCl pH 8.0 (200)          CNY                             

293013     mericon Soy Kit (24)                   CNY                             

9001919  PyroMark Q96 MD Automated, Priority PyroMark Q96 MD Automated, Priority includes the PyroMark Q96 MD Automated instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.     CNY                             

133115     NeXtalStock Sodium sulfate (200)             CNY                             

965662     QIAamp Virus BioRobot 9604 Kit (12)     For 12 x 96 nucleic acid preps: 12 QIAamp 96 Plates, RNase-free Buffers, QIAGEN Protease, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), Carrier RNA, Caps       CNY                            

9240670  Reservoir, 1000ml, AP96/196            CNY                             

9240644  External BCR, Metrologic MS9521, AP             CNY                             

9240636  Power Entry Module, AP196/98                 CNY                             

9240616  Fuse, 5.0A, FNM, AP96             CNY                             

9240607  Fiber Optic Sensor Cable, 5M, AP98                  CNY                             

9240590  Purge Solenoid, Overflow Jar, AP96/196           CNY                             

9240573  Centrifuge RT-7, Sorvall, 115V, AP96                 CNY                             

QY-10312 Anti-BMP2 (bone morphogenetic protein 2)骨形态发生蛋白-2抗体                                                 

QY-10322 Anti-BMP-7(bone morphogenetic protein-7)骨形态发生蛋白-7抗体                                                

QY-10332 Anti-BNP （Brain Natriuretic Peptide ）脑钠素/利钠肽抗体                                                  

QY-10342 Anti-BNP/Biotin （Brain Natriuretic Peptide ）生物素化脑钠肽                                                     

QY-10352 Anti-BRAF黑素瘤相关蛋白抗体                                                 

QY-10362 Anti-BRCA1（Breast cancer susceptbility gene 1）(mouse rat)乳腺癌易感基因1抗体(小鼠 大鼠)                                                        

QY-10372 Anti-BRCA1（Breast cancer susceptbility gene 1）（human）乳腺癌易感基因1抗体（人）                                                

QY-10382 Anti-BRCA2（Breast cancer susceptbility gene 2）乳腺癌易感基因2抗体                                                      

QY-10392 Anti-BrdU (Bromodeoxyuridine)溴脱氧尿苷抗体                                               

QY-10402 Anti-BrdU (Bromodeoxyuridine)溴脱氧尿苷单克隆抗体                                                    

QY-10412 Anti-BrdU (Bromodeoxyuridine)溴脱氧尿苷抗体                                               

QY-10422 Anti-BRMS-1 (Breast cancer metastasis-suppressor 1)乳腺癌转移抑制基因1抗体                                                 

QY-10432 Anti-RCA兔抗牛血清白蛋白抗体                                               

QY-10442 Anti-BTG2/TIS21(B-cell translocation gene 2)B细胞迁移基因2抗体                                                

QY-10452 Anti-BTK（Monoclonal Antibody to BTK）鼠抗人非受体型酪氨酸激酶BTK单克隆抗体                                              

QY-10462 Anti-C5a过敏毒素C5a（补体C5a）抗体                                                   【大鼠组蛋白H2b(histon-H2b)ELISA试剂盒现货】

QY-10472 Anti-CA125 （Ovarian cancer antigen）卵巢癌抗原抗体                                                 

QY-10482 Anti-CA15-3乳腺癌相关抗原抗体                                                       

QY-10492 Anti-CAD/CPAN（Caspase-Activated Deoxyribonuclease）Caspase 激活的脱氧核糖核酸酶抗体                                                  

QY-10502 Anti-Calcineurin α /PP-2B alpha 1蛋白磷酸酯酶-2B催化亚单位抗体                                                  

QY-10512 Anti-Calpain 1钙蛋白酶抗体                                               

QY-10522 Anti-Calponin 1钙调节蛋白-1抗体                                                      

QY-10532 Anti-CaMK2a（calcium/calmodulin-dependent protein kinase II alpha）钙/钙调素依赖蛋白激酶2α抗体                                                   

QY-10542 Anti-CaMK2b（calcium/calmodulin-dependent protein kinase II beita）钙/钙调素依赖蛋白激酶2b抗体                                                     

QY-10552 Anti-CAS （Cellular Apoptosis Susceptibility）细胞凋亡敏感性基因                                                

QY-10562 Anti-Caspase-1 (ICE/CASP-1/P45)天冬氨酸-胱氨酸特异性蛋白酶家族抗体                                                      

QY-10572 Anti-Caspase-10半胱胺酸蛋白酶-10抗体                                                  

QY-10582 Anti-Caspase-12半胱胺酸蛋白酶蛋白-12抗体                                                  

QY-10592 Anti-Caspase-13半胱胺酸蛋白酶蛋白-13抗体                                                  

QY-10602 Anti-Caspase-3/CPP32/SCA-1半胱胺酸蛋白酶蛋白-3抗体                                                 

QY-10612 Anti-Caspase-6 （CT）半胱胺酸蛋白酶蛋白-6抗体（C端）