1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！【大鼠高敏甲状腺素(u-T4)ELISA试剂盒现货】

ELISA应用：

ELISA检测试剂盒、ELISAKit、酶联免疫试剂盒、酶联免疫吸附测定试剂盒、酶联免疫分析试剂盒、酶免试剂盒等,比较常见的叫法是ELISA检测试剂盒、酶联免疫吸附测定试剂盒等ELISA试剂盒自从60-70年代问世以来,得到全世界科研工作者的认可及推崇,在欧美及中国获得很大的推广,尤其是国内生化领域的长足发展.Elisa生物试验是一种敏感性高,特异性强,重复性好的实验诊断方法.由于其试剂稳定、易保存,操作简便,结果判断较客观等因素,已广泛应用在免疫学检验的各领域中.ELISA检测试剂盒适用于体外定性检测人血清或血浆中的抗人类戊型肝炎（HEV）病毒IgM抗体ELISA检测

【大鼠高敏甲状腺素(u-T4)ELISA试剂盒现货】齐一生物销售：021－6034 8496；181214 53965；173021 04490  web：www.qiyibio.com

试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【大鼠高敏甲状腺素(u-T4)ELISA试剂盒现货】ELISA实验原理

试剂盒是固相夹心法酶联免疫吸附实验（ELISA）.已知浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测.先将生物素标记的抗体同时温育.洗涤后,加入亲和素标记过的HRP.再经过温育和洗涤,去除未结合的酶结合物,然后加入底物A、B,和酶结合物同时作用.产生颜色.颜色的深浅和样品中的浓度呈比例关系.

免疫测定技术的基础在于抗原抗体之间的特异结合反应,所以任何优质的诊断试剂离不开优质的原料,如抗原抗体,酶等.以前用于免疫测定的抗原通常为各种纯化抗原,而抗体则为纯化抗原免疫动物后获得的多克隆抗体和使用杂交瘤技术得到的单克隆抗体,而抗原或抗体的标记物如酶标结合物则通过各种化学合成方法制备.近年来,随着分子生物学的发展,使用基因工程方法制备各种特殊的抗原或抗体及其酶结合物等免疫测定试剂几成为现实的新一代试剂,各种新型使用的测定方法也不断出现.

HBsAg试剂特点（检测模式：临床抗体夹心法）：包被抗体为山羊多抗.多抗具有高亲和性和对抗原各种表位的反应性.酶表抗体为与HBsAg有不同结合位点的鼠复合单位,可测得HBsAg变异样品,提高检测的特异性（99.98%）提高检测的灵敏度,应用ParlEhrich学说（PEI）HBsAg标准品,对ad标准品的灵敏度为0.05ng/ml对ay标准品灵敏度为0.025ng/ml

ELISA检测试剂盒应用定性夹心免疫检测技术,用合成的HEV多肽抗原包被微孔板板条,这些多肽是中国型HEV毒株核心氨基酸序列中抗原性很强的肽段,分别来自于该毒株的开放阅读框2和开放阅读框3.将样品或标准品加入孔中并孵育,如果其中存在HEVIgM抗体,这些抗体就会与HEV的多肽抗原结合,并固定在上面,洗板除去其它非特异性抗体和样品中的其它成份.然后加入羊抗人IgM-HRP（辣根过氧化物酶）酶结合物,经第二次孵育后,酶结合物就会与次孵育结合上的HEVIgM抗体相结合,洗板除去未结合的酶结合物,加入TMB底物溶液,在第三次孵育时会发生酶-底物反应,只有那些含有HEVIgM抗体和酶结合物所形成的复合物的孔才会发生颜色变化,加入硫酸溶液终止酶和底物间的反应,并在波长:450nm处测量O.D.值,按照本HEVIgM抗体elisa试剂盒的测试标准,O.D.值大于或等于Cut-Off值的样品被认为是初试阳性.

以上资料仅供参考了解，不供于论文写作

【大鼠高敏甲状腺素(u-T4)ELISA试剂盒现货】齐一生物专业为您提供高品质，高灵敏度.高质量的酶联免疫试剂盒，齐一生物长期经营ELISA试剂盒（免费代测）及抗体、分子学生物试剂盒、金标法检测试剂盒、比色法检测试剂盒、细胞菌株、培养基、细胞因子、生化试剂、耗材等生物试剂产品。齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

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979017  MOTT 16S Primer 2 For Sequencing        CNY            

59824   EpiTect Fast DNA Bisulfite Kit (50)     CNY            

206246  Type-it Microsatellite PCR Kit (2000)       CNY            

9012742 Syringe pump outlet tubing (4.7 m)      CNY            

9012736 Peristaltic pump outlet tubing (1.0 m)      CNY            

9012725 Syringe pump outlet for Plato       CNY            

979001  PyroMark Q96 Plate (100)    "For sample analysis of DNA template prepared with magnetic beads for ID.

Magnetic bead technology instead of vacuum prep tool is used here"  CNY            

9012706 Base, auto vac, BR8000  This is the robovac base for the BR8000 I. There are 5 contacts.    CNY            

133240  NeXtalStock Sodium hydroxide (200)      CNY            

133233  NeXtalStock Sodium cacodyl. pH 7.5 (100)        CNY            

9002195 RGQ 2plex, PC, PrioPLUS, basic, no PM   "RGQ 2plex, PC Instrument

Installation of RGQ 2plex & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10 days

No Loaner available

No preventive Maintenance

Priority service provision over non-contract customers

Documented installation & repair report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"  CNY            

9002039 Rotor-Gene Q MDx 5plex HRM, PrioPLUS    "Rotor-Gene Instrument

Installation of Rotor-Gene & Introductory training

Warranty extension to 3 yrs

Regional Repair Center service, 7-10 day turn around time

Loaner available in 2 days*

Annual Inspections (1 PM per yearr)

Priority service provision over non-contract customers

Documented installation & PM report

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All cost covered (shipping, labor, parts)"  CNY            

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971540  therascreen® UGT1A1 Pyro Kit (24)       CNY            

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9002013 Rotor-Gene Q MDx 2plex HRM System       CNY            

970032  PyroMark Q24 CpG MGMT (4 x 24)  For quantification of methylation level in region 17-39 of exon 1 of the MGMT gene.  CNY            

922920  LiquiChip Filter Microplates (10)       CNY            

292023  mericon Cattle (24)     CNY            

292013  mericon Pig Kit (24)        CNY            

9001903 QIAgility HEPA/UV (PC) Priority QIAgility HEPA/UV (PC) Priority includes the QIAgility instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor ａnd travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.  CNY            

133122  NeXtalStock Potassium citrate (200)     CNY            

963141  QIAquick 96 PCR BioRobot Kit (4)    For purification of 4 x 96 PCR products: 4 QIAquick 96 Plates, Reagents, Buffers, Collection Microtubes (1.2 ml) ａnd Caps, 96-Well Microplates RB ａnd Lids, Tape Pads    CNY            

133193  NeXtalStock di-Potassium phosphate (200)        CNY            

9018555 Cover, front, top, EZ1a     CNY            

9018528 User Manual EZ1 Advanced        CNY            

37601   Qproteome Plasma Membrane Protein Kit       CNY            

9015329 O-rings, Norpren tube fixing, M48 (2)       CNY            

9015328 Seal, cap, M48 (6)      CNY            

239095  GelPilot 1 kb Plus Ladder (100) Ready-to-use DNA marker, 600µl (100lanes), 12 fragments: 100 – 10000 bp  CNY            

QY-18682    anti-Tie2（Porcine tyrosine kinase with immunoglobulin-like ａnd EGF-like domains 2）上皮生长因子样域酪氨酸激酶2                      

QY-18692    Anti-TIGAR humen (TP53- induced glycolysis ａnd apoptosis-regulator )兔抗人TIGAR蛋白抗体                       

QY-18702    Anti-TIMP-1(Tissue Inhibitor of Metalloproteinase-1)金属蛋白酶组织抑制因子-1抗体                       

QY-18712    Anti-TIMP-2(Tissue Inhibitor of Metalloproteinase-2)金属蛋白酶组织抑制因子-2抗体                       

QY-18722    Anti-TIMP-3(Tissue Inhibitor of Metalloproteinase-3)金属蛋白酶组织抑制因子-3抗体                       

QY-18732    Anti-TIMP-4(Tissue Inhibitor of Metalloproteinase-4)金属蛋白酶组织抑制因子-4抗体                       

QY-18742    Anti-TLR2 (Toll-like receptor 2)Toll样受体2抗体                       

QY-18752    Anti-TLR4 (Toll-like receptor 4)Toll样受体4抗体                       

QY-18762    Anti-TLR5/CD285 (Toll-like receptor 5)Toll样受体5抗体                     

QY-18772    Anti-TM（Thrombomodulin)血栓调节蛋白抗体                       

QY-18782    Anti-TmR2（GDNFRβ；TRN-R；GFR-alpha-2；NTNR-2）胶质细胞系源性神经营养因子受体β抗体                        【大鼠高敏甲状腺素(u-T4)ELISA试剂盒现货】

QY-18792    anti-Tn-C(tenascin-C)腱糖蛋白-C抗体                    

QY-18802    Anti-TNF-α (Mouse Anti-Human Tumor Necrosis Factor-α Monoclonal Antibody)鼠抗人肿瘤坏死因子-α单克隆抗体                       

QY-18812    Anti-TNF-α(Tumor Necrosis Factor-α)肿瘤坏死因子-α抗体                       

QY-18822    Anti-TNF-β(Tumor Necrosis Factor-β)肿瘤坏死因子-β抗体                       

QY-18832    Anti-TPⅠ （topoisomeraseⅠ）拓普西异构酶Ⅰ抗体                    

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QY-18852    Anti-TPH (Trptophan Hydroxylase)色氨酸羟化酶抗体                       

QY-18862    Anti-TPO (Thyroid Peroxidase)抗甲状腺过氧化物酶抗体                    

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QY-18892    Anti-TRADD （TNF receptor 1 associated via death domain）有死亡区的肿瘤坏死因子受体1相关蛋白抗体                     

QY-18902    Anti-TRAF1 （TNF receptor-associated factor 1）肿瘤坏死因子受体相关因子1抗体                      

QY-18912    Anti-TRAF2 （TNF receptor-associated factor 2）肿瘤坏死因子受体相关因子2抗体                      

QY-18922    Anti-TRAF3/CD40bp （CD40 binding protein）CD40结合蛋白/CD40受体相关因子1抗体                     

QY-18932    Anti-TRAF6 （TNF receptor-associated factor 6）肿瘤坏死因子受体相关因子6抗体                      

QY-18942    Anti-TRAIL/Apo2L (tumor necrosis factor-related apoptosis-inducingligand)肿瘤坏死因子相关凋亡诱导配体/凋亡素2配体抗体                     【大鼠高敏甲状腺素(u-T4)ELISA试剂盒现货】

QY-18952    Anti-Transthyretin (TTR/prealbumin)转甲状腺素蛋白抗体                      

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