价格详细资料：试剂盒价格电议，齐一生物销售：021－6034 8496；181214 53965；173021 04490,齐一生物科技（上海）有限公司提供ELISA试剂盒受到了广大研究所.中科院科研单位一致肯定和认同。齐一生物大品牌保证，倾力为国内外科研院校实验室提供*优质产品。

【人抗甲型肝炎病毒IgM抗体(anti-HAV)ELISA试剂盒现货】注意事项

1. 当混合蛋白溶液时应尽量轻缓，避免起泡。

2. 洗涤过程非常重要，不充分的洗涤易造成假阳性。

3. 一次加样时间控制在5分钟内，如标本数量多，推荐使用排枪加样。

4. 请每次测定的同时做标准曲线，做复孔。

5. 如标本中待测物质含量过高，请先稀释后再测定，计算时请*后乘以稀释倍数。

6. 在配制标准品、检测溶液工作液时，请以相应的稀释液配制，不能混淆。

7. 底物请避光保存。

8. 不要用其它生产厂家的试剂替换试剂盒中的试剂。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【人抗甲型肝炎病毒IgM抗体(anti-HAV)ELISA试剂盒现货】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【人抗甲型肝炎病毒IgM抗体(anti-HAV)ELISA试剂盒现货】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【人抗甲型肝炎病毒IgM抗体(anti-HAV)ELISA试剂盒现货】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

9238862  #Premium Cover Agreement 9604, 2PM, 1yr The Premium Cover Agreement 9604, 2 PM provides full service coverage for the BioRobot 9604. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes two annual preventive maintenance visits, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).     CNY                             

9241208  QIAsymphony SP, Premium Agreement   "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: not applicable

- QIAsense: QIAsymphony should to be connected to QIAsense for faster download of log files ａnd remote diagostic support." CNY                             

9241200  QIAxcel, Basic Agreement, no PM  "Repair service delivery send to Regional Repair Center

Service response time (repair) 7-10 days turnaround time

Periodic inspection/maintenance: 0

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: not applicable"        CNY                             

9019468  PLATE GLASS LONG SET GS                CNY                             

9233375  Prssure retaining valve for Washer Mod.                  CNY                             

9237348  AdapterPlate, W-Drive, BR8 RD v2.0                CNY                             

9237329  Upgrade, BR & 3 ext. devices w. sched.  Upgrade BioRobot with three approved integration devices using scheduling software  CNY                              【人抗甲型肝炎病毒IgM抗体(anti-HAV)ELISA试剂盒现货】

9237168  Bottle Sheath Supply                 CNY                             

9233004  Fan 24V, for BR3000 with VariTerm                   CNY                             

9237151  Power Supply            CNY                             

9237123  Upgrade App., QIAquick 96 PCR      On-site application Upgrade for standard QIAGEN QIAquick 96 including kit CNY                             

9237106  Protocol Dev./Opt., on-site (2-days)         The QIAGEN BioRobot Systems Protocol Development/Optimization On-Site Visit includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Instrumentation Service Specialist to write and/or opimize protocols for the BioRobot System. Parts, consumable chemistries, ａnd accessories not included. Custom protocols are the sole responsibility of the customer ａnd neither warrantied nor guaranteed.          CNY                             

9237097  Training, BR QIAGEN Advanced       A minimum of 4 people are required to attend. The QIAGEN BioRobot Systems Advanced Training includes three days of  training at our training facility to introduce users to advanced software topics for the  QIAGEN BioRobot Systems.  Designed for users with a basic understanding of the use ａnd programming of QIAGEN BioRobot systems who want to write complex protocols for their instrument, the advanced training starts with a brief review of basic concepts such as teaching slots ａnd racks before exploring the higher-level programming features of the QIAsoft Operating System.  Topics include the structure ａnd use of functions, using variable for programming flexibility, IF/ELSE/END boolean logic commands, ａnd parameterized pipetting using tables.  Users will write, test ａnd run protocols they have developed to demonstrate ａnd reinforce training success.       CNY                             

9236422  Power supply, replacement kit, TWI                  CNY                             

9232968  Print YZ96-Adapter-Board Plato-7            CNY                             

9236363  Reservoir XYP            CNY                             

9236353  Tubing Sample Needle to 3-Way Valve              CNY                             

9236329  2-Way Valve (Sheath or vent)           CNY                             

9236160  Potentionmeter, rotary, kit, RapidPlate           CNY                             

9236154  Online Fill Pump RP           CNY                             

9236126  QIAGEN Multiwell-Plate Holder , silver   Holder for accommodating multiwell plates on the Microplate station of the BioRobot MDx worktable         CNY                             

9236120  Runner, Drawer, BR8 RD                   CNY                             

9235790  Ramp, Locking, Drawer, BR8 RD              CNY                              【人抗甲型肝炎病毒IgM抗体(anti-HAV)ELISA试剂盒现货】

9230745  Z-Lead screw for Plato 7           CNY                             

9235690  Cable X-Main, RCS1           CNY                             

9235572  Shaker Tool, RCS1              CNY                             

9230314  Cable, RS232, syringe pump, 225mm                CNY                             

9230283  Cable, Interface, X-main to Washer                   CNY                             

5000-1302        Capacitor 2UF, SKR1                  CNY                             

5000-1284        DML 2000 - Power Cord, 220V D Type (GB)              CNY                             

5000-1254        Power Supply PCB, DML2                  CNY                             

5000-1250        CPU DML 2000 ASSRY Kit, DML2              CNY                             

6000-3401        RCS Upgrade Package               CNY                             

QY-x1442 狗肾细胞MDCK                                                     【人抗甲型肝炎病毒IgM抗体(anti-HAV)ELISA试剂盒现货】

QY-x1443 大鼠肝星形细胞CFSC-8B                                                     

QY-x1444 大鼠肝星形细胞CFSC-2G                                                     

QY-x1445 大鼠肝星形细胞CFSC-3H & 5H                                                   

QY-x1446 大鼠肝上皮样干细胞WB-F344                                                   

QY-x1447 鼠肌原细胞C2C12                                               

QY-x1448 大鼠肺上皮Ⅱ型细胞RLE-6TN                                                    

QY-x1449 小鼠肺成纤维细胞L929                                                      

QY-x1450 大鼠胸主动脉平滑肌细胞，ATCC：A10VSMC                                                   

QY-x1451 小鼠胚成纤维包装细胞PA317                                                    

QY-x1452 小鼠胚成纤维包装细胞ψ2                                                

QY-x1453 叙利亚仓鼠肾细胞BHK-21                                                 

QY-x1454 BHK                                               

QY-x1455 转S9基因仓鼠卵巢细胞ZA1                                                      

QY-x1456 转S9基因仓鼠卵巢细胞ZA6                                                      

QY-x1457 大鼠巨噬细胞MC                                                

QY-x1458 非洲绿猴肾细胞COS-1                                                 【人抗甲型肝炎病毒IgM抗体(anti-HAV)ELISA试剂盒现货】

QY-x1459 非洲绿猴肾细胞COS-6                                                

QY-x1460 猴肾细胞系BSC-1                                                

QY-x1461 小鼠肾小球系膜细胞MES-13                                                      

QY-x1462 小鼠肾系膜细胞MC53                                                

QY-x1463 大鼠肾成纤微细胞NRK-49F                                               

QY-x1464 小鼠皮肤细胞JB6-C30                                                 

QY-x1465 小鼠皮肤细胞JB6-C41                                                 

QY-x1466 小鼠前成骨细胞MC-3T3                                                     

QY-x1467 小鼠成骨细胞系MC3T3-E1                                                 

QY-x1468 鼠胚成骨细胞3T3-E1                                                  

QY-x1469 大鼠肾小管上皮细胞，ATCC：CRL-6509NRK                                                     

QY-x1470 大鼠肾细胞NRK-52E                                                    

QY-x1471 小鼠饲养层上皮细胞HPT-8                                                

QY-x1472 LLC:Leuwis肺癌细胞