规格:小规格48T.大规格96T

样本标准：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：酶联免疫分析（ELISA）

运输保存：2~8°贮存.保质期：6个月

试验所需自备物品:

1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

【人磷酸果糖激酶(PFK)ELISA试剂盒现货】齐一生物主要经营进口原装Elisa试剂盒,检测试剂种类齐全,价格实惠！购买齐一生物产品的新老客户都可享免费代测实验服务,全程提供技术支持.如需其它Elisa试剂盒说明书及产品价格,可来电咨询齐一生物销售：021-6034 8496；181214 53965；173021 04490

试剂盒特点

一、高效、灵敏、特异的抗体；

二、稳定的重复性和可靠性；

三、吸附性能好,空白值低,孔底透明度高的固相载体；

四、适用血清、血浆、组织匀浆液、细胞培养上清液、尿液等等多种标本类型；

试剂盒可概括四个方面：

1、免疫酶染色各种细胞内成份的定位.

2、研究抗酶抗体的合成.

3、显现微量的免疫沉淀反应.

4、定量检测体液中抗原或抗体成份.

【人磷酸果糖激酶(PFK)ELISA试剂盒现货】ELISA试剂盒为您节省实验经费.

elisa试剂盒回收率是反应待测物在样品分析过程中的损失的程度,损失越少,回收率越高,如果作标液1PPM,就是1毫克/升,而作出标准数据为0.99毫克/升,就是说你的回收率是99%,这个与真实成分有密切的关系,说明方法的准确度.比如水中总无机氯含量测定,样品水中含有无机氯20mg/L,取100mL被测水样品,加入0.1mL浓度为10mg/mL的含无机氯标准样品,测定时忽略体积变化,如果测定出样品中无机氯为2.98mg/L,则认为回收率为99%.

【人磷酸果糖激酶(PFK)ELISA试剂盒现货】试剂盒制备方法包括以下步骤：

1)抗原表位的计算机筛选；

2)抗原的制备；

3)血清的采集；

4)间接ELISA方法的建立；

5)间接ELISA方法的敏感性和特异性验证；

6)试剂盒的稳定性验证；

7)对大量样本进行检测.该方法简单、快速、灵敏度高、特异性强、稳定性合格、重复性好.应用本发明制得的试剂盒能有效检出样本含量.适用于大批量样本的检测

试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

【人磷酸果糖激酶(PFK)ELISA试剂盒现货】齐一生物【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

9020586  Power Supply, 24V 60W, DML 3000           CNY                           

9013314  Shaker adapter, 192-tube, 5 ml             CNY                           

9013211  Cabling kit, data, BR8000             CNY                            【人磷酸果糖激酶(PFK)ELISA试剂盒现货】

9011747  Worktable left, BR8000 v1.01              CNY                           

132568    NeXtal Stock Methoxy-PEG 5000 (50) 30% w/v Methoxypolyethylene glycol 5000 - 50mL (4 x 12,5 ml tube)      CNY                           

800311    Custom Primer              CNY                           

766134    PAXgene Tissue miRNA Kit (50)          CNY                           

203443    HotStarTaq Master Mix Kit (250)  "3 x 0.85 ml HotStarTaq Master Mix* containing 250 units HotStarTaq DNA Polymerase total, 2 x 1.7 ml distilled water

- premixed solution for high PCR specificity

- HotStarTaq Master Mix, HotStarTaq DNA Polymerase, several kit sizes from 250 u up to 2500 u available" CNY                           

201901    dNTP Mix, PCR Grade, 10mM, 4x200µl      "dNTP Mix contains dATP, dCTP, dGTP, ａnd dTTP in water (pH 7.5), 10mM each.

for 800 reactions based on 50ul PCR (0.2uM each dNTP)" CNY                           

201209    Taq DNA Polymerase (25000)      "100 x 250 units Taq DNA Polymerase, 100 x 1.2ml PCR Buffer, 100 x 2.0ml Q-Solution, 100 x 1.2ml MgCl2

NEW! Now with 100 x 1,2 ml CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."  CNY                           

200203    TopTaq DNA Polymerase (250)   "1 x 250U TopTaq DNA Polymerase, 10x TopTaq PCR Buffer, 1x CoralLoad Concentrate, 5x Q-solutions, 25mM MgCl2

For highly reliable end-point PCR with unrivalled ease-of-use

The first fridge storage enzyme"   CNY                           

180462    GeneRead DNA Library L Core Kit (12)              CNY                           

159916    Generation Capture Column Kit (300)          CNY                           

159104    QIAsafe DNA Tubes (50)             CNY                           

158902    RBC Lysis Solution(450 ml)          CNY                           

678791    ipsogen MLL-AF9 type B e8e9 Standards            CNY                           

678391    ipsogen MLL-AF4 e9e5 Standards        CNY                           

34860     Anti-GST Antibody (100µg)         CNY                           

9021057  Door Assy, Lower Right, DCU             CNY                            【人磷酸果糖激酶(PFK)ELISA试剂盒现货】

9021056  Door Assy, Lower Left, DCU              CNY                           

9021002  Belt, Y Drive, DCU        CNY                           

9020994  De-Capper Assembly, DCU           CNY                           

9020931  Fixation angles, vac.pump v2.0, BR8000             CNY                           

9020906  HEPA filter system, SMC, QGX           CNY                           

9020704  Lid frame with hinge C1x             CNY                           

9020638  Baseboard, side, Cabinet Qsym            CNY                           

9020634  Panel, left, Cabinet Qsym             CNY                           

9020626  Holder, BC scanner, Cabinet Qsym              CNY                           

9020333  Fuse - Thermal (5), RGQII          CNY                           

9017687  AUTOPURE Pro K Pump             CNY                           

9017602  Pump, MultiFlow Disp, BR8 RD          CNY                           

9017503  Bearing, teflon, white (5), TR              CNY                           

9017257  BR 8000, Protein Purification Package         CNY                           

QY-x2062       MPC-83 胰腺腺泡细胞癌 1640+10%胎牛血清 体外培养株                                    

QY-x2063       MPC-83 胰腺腺泡细胞癌 瘤株                                    

QY-x2064       RIN-m5F β胰岛素瘤 1640+10%胎牛血清 体外培养株                                   

QY-x2065       MA891 乳腺癌 瘤株                                      

QY-x2066       TA2 自发高乳腺癌 瘤株                                        【人磷酸果糖激酶(PFK)ELISA试剂盒现货】

QY-x2067       U14 宫颈癌 瘤株                                   

QY-x2068       YAC-1 淋巴瘤 1640+10%胎牛血清 体外培养株                                       

QY-x2069       Nb2－11 淋巴瘤 体外培养株                                       

QY-x2070       C26 结肠癌 瘤株                                   

QY-x2071       G422 神经胶质瘤 瘤株                                        

QY-x2072       B16 黑色素瘤 1640+10%胎牛血清 体外培养株                                       

QY-x2073       B16 黑色素瘤 瘤株                                       

QY-x2074       C3 白血病 体外培养株                                         

QY-x2075       L1210 淋巴白血病 1640+10%胎牛血清 体外培养株                                       

QY-x2076       L1210 淋巴白血病 瘤株                                       

QY-x2077       SP2/0 骨髓瘤 1640+10%胎牛血清 体外培养株                                        

QY-x2078       NS-1 骨髓瘤 1640+10%胎牛血清 体外培养株                                         

QY-x2079       P388D 淋巴母细胞瘤 1640+10%胎牛血清 体外培养株                                   

QY-x2080       Kert-3 肾癌 瘤株                                    

QY-x2081       TC-1 HPVl6 E6、E7和ras基因共转化的C57BL／C(H-2b)小鼠肺上皮细胞 1640+10%胎牛血清体外培养株                                    

QY-x2082       W256 肉瘤(Wistar) 瘤株                                        【人磷酸果糖激酶(PFK)ELISA试剂盒现货】

QY-x2083       PC-12 嗜铬细胞瘤 1640+10%胎牛血清+5%马血清 体外培养株                                    

QY-x2084       [编辑本段]细胞库部分细胞系目录（遗传和正常细胞）                                   

QY-x2085       细胞名称 组织类型 培养条件                                     

QY-x2086       HIH 3T3 小鼠胚胎成纤维细胞 1640+10%胎牛血清                                         

QY-x2087       L929 小鼠成纤维细胞 1640+10%胎牛血清                                       

QY-x2088       HSC-T6 大鼠肝星状细胞 1640+10%胎牛血清                                   

QY-x2089       L-02 人肝细胞 1640+10%胎牛血清                                    

QY-x2090       SRA01/04 人晶体上皮细胞 MEM+非必需氨基酸＋10%胎牛血清                                   

QY-x2091       CHO 中国仓鼠卵巢细胞（野生型） 1640+10%胎牛血清                                        

QY-x2092       IEC-6 大鼠小肠隐窝上皮细胞 DMEM+10%胎牛血清