齐一生物销售：021－6034 8496；181214 53965；173021 04490

1。我必须运行所有的标准和样品的重复？

是的，重复的运行，以监测测定的精度和提高检测结果的信心。

2。我必须在一次运行所有的样品吗？

不，每个试剂盒采用stripwell微孔板。这允许用户在不同的时间来分析不同数量的样品。

3。什么类型的可重复性的结果，得到与分析？

每一个工具都有一个包含一个典型数据的图表的手册。在运营商的任何变化，移液和洗涤技术，培养时间、温度、试剂盒的年龄可以引起变化的结果。每个用户都应该得到自己的标准曲线。

4。是否有可能储存的试剂比表示？

在不推荐使用条件下的部件组件的存储，以保证测试的正确性能。

5。我应该如何储存我的样品？

样品应保存在-20°C或更低的温度。长期储存，建议冻结他们在- 70余。

6。我可以修改协议吗？

BG的ELISA试剂盒进行了优化，提供*佳的结果。修改的格式或协议可能会给出错误的结果。

7。我可以使用一个不建议在试剂盒插入的样本类型吗？

该试剂盒已被验证在试剂盒插入的样品类型。未经检验的样品类型。为进一步信息的联系技术服务。

8。我的样本产生的值，在动态范围内的测定。我可以使用这些值吗？

建议只有在标准曲线的范围内下降的样本值。标准曲线的范围内的值一般是非线性的，这可能导致不正确的外推值。产生值高于标准的样品应为（进一步）稀释和重复。如果样品低于该测定的范围内，该样品被认为是不可检测的。

9。我每次都要运行一个空白或零标准吗？

是的，这些都是必需的计算，并反映任何微妙但显着的性能变化，从一天到一天，检测到检测。当排除特定的检测问题的来源时，它们也非常有帮助。

10。我能改变我在检测中使用的样本量吗？

不建议你改变卷由于BG工具被设计为*佳的性能在给定的体积

11。可以使用不同的组件组件吗？

每一个组件包含有特定批号的组件，以确保所有的组件都进行优化，以及与组件中的所有其他组件。对这些特定的地段进行质量检验。这是从来没有建议使用你自己的组件或组件从其他包或供应商。

12。我的标准曲线看起来很好，但我没有得到一个信号，我的样本，当我预期，为什么？

样品不包含被分析物。一个矩阵的效果可能是掩盖检测。确保推荐的稀释液在试剂盒插入后按规定进行。如果建议稀释，请检查是否正确进行稀释。过稀释会导致样品在标准曲线的范围内下降。

13。你怎么推荐我洗盘子？

如果您使用的是一个自动平板洗衣机，我们建议定期检查校准，并将该系统在洗涤前冲洗缓冲液冲洗。手动洗衣机也是一样的。一个中继器或洗瓶也可以使用。用户应小心，以确保所有的内容都是送气和板拍干的无尘纸。【人磷酸肌醇3激酶(PI3K)ELISA试剂盒厂家】

14。我需要用一个盘子吗？

可靠的结果可以得到无板振动，但外径的一般会低于那些使用平板振动器。

15。为什么我要用波长校正450-570nm之间？

对于酶联免疫吸附试验，在双波长读的是做正确的光密度贡献的塑料以及，灯和光学波动。

16。如果我提我的样本，我还需要遵循推荐的稀释液试剂盒中插入了吗？

提取过程中所需的样本稀释量将受到影响的纯化和浓度的影响的协议使用。稀释或浓缩的金额将由*终用户决定。

17。什么是预期的分析物浓度，我应该找到？

一个给定的分析物的量可能会有所不同，不仅从物种的物种，但也组织和细胞来源。这个信息的*佳来源是目前的文献，很容易通过互联网在多个科学数据库访问。

18。我的光密度有点高（或较低），比那些在手册中，与我的试剂盒来了。为什么？

光学密度受影响

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【人磷酸肌醇3激酶(PI3K)ELISA试剂盒厂家】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【人磷酸肌醇3激酶(PI3K)ELISA试剂盒厂家】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【人磷酸肌醇3激酶(PI3K)ELISA试剂盒厂家】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

9243110  CD-RW, Black, AP196/98           CNY                             

9242712  Validation Support, 2d QIAcube       The QIAGEN QIAcube Guided Validation includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist. A QIAGEN specialist will provide an introduction to the QIAcube system ａnd the DNA Investigator silica chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on QIAcube operation.  The Guided Validation service includes experimental design consulation, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support.  Please indicate which protocols to include in the validation (QIAamp, QIAamp DNA Investigator, MinElute PCR Cleanup, etc.)      CNY                             

9019226  Fan - rear 92x92, PyroQ24                 CNY                             

9019220  PCB - Distribution robot, PyroQ96MD               CNY                             

1066843  PG Influenza A RT-PCR Assay (48) LE Z              CNY                             

1066848  NDV Univ. RT-PCR REAGENT LE                 CNY                             

1066854  AIV-H9 RT-PCR REAGENT LE             CNY                             

1060992  Buffer MW1 (77ml)           CNY                             

1061059  Sample Preparation Reagents, 8ml                   CNY                             

1061080  NG Quantification Standards            CNY                             

1061113  PG AIV-H7 RT-PCR Assay (48) Z                  CNY                             

9016568  Plate Grippers, RCS1                  CNY                              【人磷酸肌醇3激酶(PI3K)ELISA试剂盒厂家】

9016536  Transportation box , BioSprint 15             CNY                             

9016535  Transportation box , Twister II                   CNY                             

9016533  Transportation box , RapidPlate onlf.                CNY                             

9016530  Transportation box 1, LiquiChip                 CNY                             

9016493  BR MDx DSP Service, PRO                 CNY                             

9016325  Washer head with holder , P 13xx            CNY                             

9019185  PCB - Front, PyroQ96MD          CNY                             

9011902  Upgrade, tip change system             CNY                             

9011824  Z-Modul; Tip-Adapter 1100µl; BR8000            CNY                             

9237033  Cable, Liquid Sensor, BR8 RD v1.0            CNY                             

9236960  Probe Holder, Body, BR8 RD             CNY                             

9019144  Fan - rear 92x92, PyroQ96MD                   CNY                             

9019113  PCB - Camera, PyroQ96ID                 CNY                             

9019075  PyroMark NDT Holder, PyroQ96MD        Re-usable holder for tips, Nucleotide Dispensing Tips ａnd Reagent Dispensing Tips, for use in PyroMark Q96 MD.  CNY                             

9019010  Motor M, EZ1xl                 CNY                             

9018953  Reagent Block, 16x0.2ml/2ml PCR/Flat-Bas              CNY                              【人磷酸肌醇3激酶(PI3K)ELISA试剂盒厂家】

9018938  Adapter, Light Cycler 480 384-Well Plate                  CNY                             

9018935  Adapter, reagent tube SBS Plate              CNY                             

9015837  Upg. Kit, Y6L Connection, BR8 (ISI21)               CNY                             

9015689  Fittings (10), M6/3.3 mm, white                CNY                             

9015605  Upgrade, LiquiChip IS 2.x, PLUS                 CNY                             

QY-x2279 H-4-II-E 大鼠肝细胞瘤细胞                                                

QY-x2280 H9 人急性淋巴母细胞白血病细胞                                                    

QY-x2281 HAC-84 人肺泡癌细胞                                                

QY-x2282 Hacat 人永生化角质细胞                                                   

QY-x2283 HAL-01 人急性混合型白血病细胞                                                     

QY-x2284 HAN 人羊膜细胞                                                 

QY-x2285 H-bc 人膀胱癌细胞                                                     

QY-x2286 HBF 人膀胱组织来源细胞                                                  

QY-x2287 NRK-52E 大鼠肾细胞DMEM培养基，添加4 mM L-谷氨酰胺，含1.5 g/L 碳酸氢钠4.5 g/L 葡萄糖, 95%; 小牛血清, 5%                                                        

QY-x2288 NRK大鼠肾细胞含1.5g/L 碳酸氢钠， 4.5g/L 葡萄糖， 4mM L-谷氨酰胺的DMEM，90%；胎牛血清或新生牛血清，10%。 ： -                                                  

QY-x2289 NIH/3T3 鼠成纤维细胞系 含1.5g/L 碳酸氢钠， 4.5g/L 葡萄糖，4mM L-谷氨酰胺的DMEM，90%；BCS，10%。 ： 与原始的...                                                  

QY-x2290 NCI-N87 [N87]人胃癌细胞 RPMI 1640 medium with 2 mM L-glutamine adjusted to contain ...                                                       【人磷酸肌醇3激酶(PI3K)ELISA试剂盒厂家】

QY-x2291 NCI-H292人肺癌细胞(淋巴结转移)含2mM L-谷氨酰胺，10mM HEPES，1mM 丙酮酸钠，4.5g/L 葡萄糖，1.5g/L ...                                               

QY-x2292 NAMALWA 人Burkitt's淋巴瘤细胞B 淋巴细胞；Burkitt淋巴瘤形态： 淋巴母细胞含1.5g/L 碳酸氢钠， 4.5g/L 葡萄糖， 10mM HEPE...                                                    

QY-x2293 MRC-5 人胚肺细胞 含1.0mM丙酮酸钠，0.1mM非必需氨基酸和1.5克/升碳酸氢钠的EMEM，90%；胎牛血清，10%。 ： MRC-5细胞系来自...                                                        

QY-x2294 MGC80-3 人胃癌细胞RPMI 1640+10%胎牛血清或新生牛血清 ： 从一位53岁 性原发性胃低分化粘液腺癌患者建立。                                                       

QY-x2295 MFC小鼠胃癌细胞 RPMI 1640+10%胎牛血清或新生牛血清 ： 源自615小鼠。狗肾细胞 MDCK (NBL-2) 询价 ATCC                                                        

QY-x2296 MDCK (NBL-2) 狗肾细胞含 1.5g/L 碳酸氢钠，0.1mM 非必需氨基酸，1.0mM 丙酮酸钠，2mM L-谷氨酰胺和Earle'...                                                        

QY-x2297 MDCK犬肾细胞系添加2mM L-谷氨酰胺，1.0mM丙酮酸钠，0.1mM非必需氨基酸，1.5g/L碳酸氢钠的EMEM，90%；FBS，10%。 ： 1...                                                   

QY-x2298 MDBK (NBL-1) 牛肾细胞 含 1.5g/L 碳酸氢钠，0.1mM 非必需氨基酸，1.0mM 丙酮酸钠，2mM L-谷氨酰胺和Earle&#39...                                                  

QY-x2299 MDA-MB-453 人乳腺癌细胞 L-15+10%胎牛血清 ： 从胸水中建立的人乳腺癌细胞株。                                                

QY-x2300 MDA-MB-231 人乳腺癌细胞转移性胸膜渗出液形态：Leibovitz’s L15培养液，添加10%胎牛血清。 ： MDA-MB-231来...                                                       

QY-x2301 MCF7人乳腺癌细胞EMEM加1.0mM丙酮酸钠，0.1mM非必需氨基酸和1.5克/升碳酸氢钠，添加0.01毫克/毫升牛胰岛素和10%胎牛血... 恒河猴肾细胞 LLC-MK2 询价 ATCC                                                

QY-x2302 LLC-MK2 恒河猴肾细胞 含1.68g/L碳酸氢钠的199培养基，99%；新生牛血清，1%。 ： LLC-MK2细胞株源自六只成年绿猴的肾细胞悬液。 据报...                                                     

QY-x2303 LLC小鼠肺癌细胞 DMEM+10%胎牛血清或新生牛血清 ： 小鼠Lewis肺癌细胞                                               

QY-x2304 L929小鼠成纤维细胞RPMI 1640+10%胎牛血清或新生牛血清 ： 亲本L的亚克隆，是*早建立的连续培养物。 L细胞源自100天的...                                                        

QY-x2305 L6 大鼠成肌细胞含4mM L-谷氨酰胺，1.0mM 丙酮酸，4.5g/L 葡萄糖和1.5g/L碳酸氢钠的DMEM 90%；胎牛血清 10%。 ...                                                     

QY-x2306 Jurkat人T淋巴细胞白血病细胞 RPMI-1640 10%FBS ： 细胞株来源于一个14岁 孩的外周血。 经佛波酯和外源凝集素或抗T3单克...                                                       【人磷酸肌醇3激酶(PI3K)ELISA试剂盒厂家】

QY-x2307 Huh-7 人肝癌细胞 DMEM 10%FBS ： 据说产甲胎蛋白，胰酶α抗体，血浆铜蓝蛋白，纤维蛋白原，纤维粘连蛋白等。                                                      

QY-x2308 HT-29 人结肠癌细胞 McCoy’s 5a 10%FBS ： 1964年，该细胞系由J.Fogh 用移植培养方法和含15%FBS的F12培...                                                      

QY-x2309 HT-1080 人纤维肉瘤细胞系DMEM 10%FBS ： 这株细胞含有活化的N-ras癌基因。                                              

QY-x2022    CEM 白血病 1640+10%胎牛血清                    

QY-x2023    K562 慢性髓原白血病 1640+10%胎牛血清                       

QY-x2024    HPB-ALL T细胞白血病 1640+10%胎牛血清                       

QY-x2025    HL-60 白血病 1640+10%胎牛血清                      

QY-x2026    Daudi B淋巴细胞瘤 1640+10%胎牛血清                     

QY-x2027    SK-BR-3 乳腺腺癌 1640+15%胎牛血清                      

QY-x2028    MCF-7 乳腺腺癌 1640+10%胎牛血清                    

QY-x2029    MCF-7/Adr 乳腺腺癌/阿霉素耐药株 1640+10%胎牛血清                       

QY-x2030    A2780 卵巢癌 1640+10%胎牛血清