1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！【人副流感病毒（HPIV）抗原ELISA试剂盒厂家】

ELISA应用：

ELISA检测试剂盒、ELISAKit、酶联免疫试剂盒、酶联免疫吸附测定试剂盒、酶联免疫分析试剂盒、酶免试剂盒等,比较常见的叫法是ELISA检测试剂盒、酶联免疫吸附测定试剂盒等ELISA试剂盒自从60-70年代问世以来,得到全世界科研工作者的认可及推崇,在欧美及中国获得很大的推广,尤其是国内生化领域的长足发展.Elisa生物试验是一种敏感性高,特异性强,重复性好的实验诊断方法.由于其试剂稳定、易保存,操作简便,结果判断较客观等因素,已广泛应用在免疫学检验的各领域中.ELISA检测试剂盒适用于体外定性检测人血清或血浆中的抗人类戊型肝炎（HEV）病毒IgM抗体ELISA检测

【人副流感病毒（HPIV）抗原ELISA试剂盒厂家】齐一生物销售：021－6034 8496；181214 53965；173021 04490  web：www.qiyibio.com

试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【人副流感病毒（HPIV）抗原ELISA试剂盒厂家】ELISA实验原理

试剂盒是固相夹心法酶联免疫吸附实验（ELISA）.已知浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测.先将生物素标记的抗体同时温育.洗涤后,加入亲和素标记过的HRP.再经过温育和洗涤,去除未结合的酶结合物,然后加入底物A、B,和酶结合物同时作用.产生颜色.颜色的深浅和样品中的浓度呈比例关系.

免疫测定技术的基础在于抗原抗体之间的特异结合反应,所以任何优质的诊断试剂离不开优质的原料,如抗原抗体,酶等.以前用于免疫测定的抗原通常为各种纯化抗原,而抗体则为纯化抗原免疫动物后获得的多克隆抗体和使用杂交瘤技术得到的单克隆抗体,而抗原或抗体的标记物如酶标结合物则通过各种化学合成方法制备.近年来,随着分子生物学的发展,使用基因工程方法制备各种特殊的抗原或抗体及其酶结合物等免疫测定试剂几成为现实的新一代试剂,各种新型使用的测定方法也不断出现.

HBsAg试剂特点（检测模式：临床抗体夹心法）：包被抗体为山羊多抗.多抗具有高亲和性和对抗原各种表位的反应性.酶表抗体为与HBsAg有不同结合位点的鼠复合单位,可测得HBsAg变异样品,提高检测的特异性（99.98%）提高检测的灵敏度,应用ParlEhrich学说（PEI）HBsAg标准品,对ad标准品的灵敏度为0.05ng/ml对ay标准品灵敏度为0.025ng/ml

ELISA检测试剂盒应用定性夹心免疫检测技术,用合成的HEV多肽抗原包被微孔板板条,这些多肽是中国型HEV毒株核心氨基酸序列中抗原性很强的肽段,分别来自于该毒株的开放阅读框2和开放阅读框3.将样品或标准品加入孔中并孵育,如果其中存在HEVIgM抗体,这些抗体就会与HEV的多肽抗原结合,并固定在上面,洗板除去其它非特异性抗体和样品中的其它成份.然后加入羊抗人IgM-HRP（辣根过氧化物酶）酶结合物,经第二次孵育后,酶结合物就会与次孵育结合上的HEVIgM抗体相结合,洗板除去未结合的酶结合物,加入TMB底物溶液,在第三次孵育时会发生酶-底物反应,只有那些含有HEVIgM抗体和酶结合物所形成的复合物的孔才会发生颜色变化,加入硫酸溶液终止酶和底物间的反应,并在波长:450nm处测量O.D.值,按照本HEVIgM抗体elisa试剂盒的测试标准,O.D.值大于或等于Cut-Off值的样品被认为是初试阳性.

以上资料仅供参考了解，不供于论文写作

【人副流感病毒（HPIV）抗原ELISA试剂盒厂家】齐一生物专业为您提供高品质，高灵敏度.高质量的酶联免疫试剂盒，齐一生物长期经营ELISA试剂盒（免费代测）及抗体、分子学生物试剂盒、金标法检测试剂盒、比色法检测试剂盒、细胞菌株、培养基、细胞因子、生化试剂、耗材等生物试剂产品。齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

9230281 Cable, Interface, Plato-Syringe Pump        CNY            

9235253 Trough-Rack spezial     CNY            

9230197 Plug f. dilutor valve inlet     CNY             【人副流感病毒（HPIV）抗原ELISA试剂盒厂家】

9230059 Rack for reagents, Trough(for 3 troughs)        CNY            

9238674 Bracket for grey wedge      CNY            

9238652 Interference suppression capacitor      CNY            

9238636 Gear motor, lid opening     CNY            

9242027 Repair fee, Depot Repair, Palm Cycler       CNY            

9241822 Pyro Q24, Full Agreement    """Repair service delivery: send to Regional Repair Center

Service response time (repair): 7-10 days turnaround time

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

 or Repair Center Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: yes, included (provision time 2-3 days)

Transportation cost coverage: yes, full coverage""" CNY            

9241819 Pyro Q96 ID, Basic Agreement    "Repair service delivery: onsite

Service response time (repair): 5 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"   CNY            

9240975 PCB, control, sample tracking, Qsym     CNY            

9240969 PCB, carriersens, sample tracking, Qsym     CNY            

9240682 Dump Tray, Overflow Control, AP98       CNY            

9240789 Update,Unload Valve Assy, AP196/98      CNY            

9240815 #Basic Cover Agreement EZ1 DSP, 1YR     CNY            

9240775 Sample Rack, AP     CNY            

6000-5017   MST Conv.Rack&Lid (Silver - 2 pos. cal.)        CNY            

9241762 QIAgility, Basic Agreement  "Repair service delivery: onsite

Service response time (repair): 5 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) or PC repair provision: not included

Transportation cost coverage: not applicable"   CNY            

9241380 24VDC Adaptor Cable, Microlynx, AP      CNY             【人副流感病毒（HPIV）抗原ELISA试剂盒厂家】

9240758 Update, Bracket, Shaker Bimba, AP98     CNY            

9243552 Target Prep, PM Service "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"   CNY            

9243384 Cooling adapter, SBS Universal, Qsym        CNY            

9243116 Lee Sample Valve Kit, LC        CNY            

9243234 HC2, PM Agreement   The Preventive Maintenance (PM) service ensures the correct operation of the equipment. During the PM, all the instrument modules are inspected by a qualified QIAGEN service technician. The Preventive Maintenance is performed under the manufacturer guidelines ａnd documented according to GLP / GMP CNY            

9243044 Set, brackets, transparent panel, Qsym      CNY            

9242752 Kit, upg-x/y-drive, portal, Qsym        CNY            

9242711 Validation Support, 2d, QIAsymphony The QIAGEN QIAsymphony Guided Validation Support service includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Applications Specialist.  A QIAGEN specialist will provide an introduction to the QIAsymphony system ａnd the DNA Investigator silica chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on QIAsymphony instrument operation.  The Guided Validation service includes experimental design consultation, internal validation documentation support, ａnd QIAGEN sponsored data templates. The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support. Please indicate which protocols to include in the validation (Casework200, Casework500, Reference200, etc.).    CNY            

9242203 Cooling adapter, SBS Universal, Qsym        CNY            

9242198 Lid Sealing Ring, Eppendorf, AP196/98       CNY            

5000-1402   Latch Front Assembly, VTX2      CNY            

5000-1315   Rotary Shaker - Spring Single       CNY            

5000-1308   Sensor Speed, SKR1      CNY            

5000-1280   Bottom Feet W Screws, DML2      CNY            

QTR-919 癌胚抗原(CEA)检测卡 1T  20T、50T/盒            

QTR-920 前列腺特异性抗原(PSA)检测卡 1T  20T、50T/盒            

QTR-921 布病阻断ELISA抗体检测试剂盒                       

QTR-922 贾弟虫抗原检测试纸条                        【人副流感病毒（HPIV）抗原ELISA试剂盒厂家】

QTR-923 A型轮状病毒ELISA抗原检测试剂盒                    

QTR-924 A型轮状病毒抗原检测试纸条                      

QTR-925 西尼罗河热阻断ELISA抗体检测试剂盒                     

QTR-926 小球隐孢子虫检测试剂条                     

QTR-927 A型流感阻断ELISA抗体检测试剂盒                    

QTR-928 猪胸膜肺炎放线杆菌iELISA抗体检测试剂盒（10,12型）                     

QTR-929 猪胸膜肺炎放线杆菌iELISA抗体检测试剂盒（1,2,9,11/3,6,8,15/4,5,7型）                       

QTR-930 猪伪狂犬病病毒(全)抗体检测试剂盒                       

QTR-931 猪伪狂犬gB-ELISA抗体检测试剂盒                    

QTR-932 猪伪狂犬gE-ELISA抗体检测试剂盒                    

QTR-933 非洲猪瘟抗原检测ELISA试剂盒                       

QTR-934 非洲猪瘟cELISA抗体检测试剂盒                      

QTR-935 非洲猪瘟病毒抗体检测试纸条                     

QTR-936 猪布病iELISA抗体检测试剂盒                    

QTR-937 副猪嗜血杆菌iELISA抗体检测试剂盒                      

QTR-938 流感iELISA抗体检测试剂盒                       【人副流感病毒（HPIV）抗原ELISA试剂盒厂家】

QTR-939 双抗夹心法猪圆环病毒抗原检测试剂盒                     

QTR-940 PCV-2 IgG/IgM抗体检测试剂盒                    

QTR-941 PCV-2 IgG抗体检测试剂盒                    

QTR-942 猪丹毒iELISA抗体检测试剂盒                    

QTR-943 猪肺炎支原体ELISA抗体检测试剂盒                       

QTR-944 猪细小病毒iELISA抗体检测试剂盒                    

QTR-945 猪细小病毒ELISA抗原检测试剂盒                     

QTR-946 猪传染性胃肠炎/猪冠状病毒检测试剂盒（Porcine Coronavirus(PRCV/TGEV)）                      

QTR-947 猪传染性胃肠炎和冠状病毒二联抗体检测试剂盒                     

QTR-948 猪冠状病毒ELISA抗体检测试剂盒                     

QTR-949 猪传染性胃肠炎ELISA抗体检测试剂盒