1.购买我公司任意ELISA试剂盒指标享受免费代测，并提供技术指导服务！

二、实验材料与试剂配制：

1. 仪器与材料：酶标仪（使用前预热30分钟），微量加液器、吸头、蒸馏水或去离子水，滤纸。

2. 缓冲液使用：加5ul  的缓冲液于50ul  的样品中，如果样品量不够或者不确定，缓冲液和样品的混合比例不要小于1:10即可混匀，静置1小时备用（如果标本是血清或者血浆，此步骤忽略）。

3. 洗液的配制：按1:100的比例配制洗液备用。

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人轮状病毒抗原(RVAg)ELISA试剂盒现货】三. 仔细阅读说明书

1.检查试剂盒标签上的有效期。如果超过有效期，请勿使用。

2. 按说明书确定所有试剂齐全（数量、体积）

3. 标本制备要规范，每份标本体积要按2-3个复孔以上的量制备（贮存），尽量分装做备份。

4.短期内无法实验者，注意低温保存

5.准备好所有实验额外所需物品，（比如移液器，试管，清洗器，酶标仪）

6. 按说明书将所用试剂平衡至室温，根据检测标本数量确定所需试剂的量

7.检查试剂盒内每种试剂的贮存条件，保证所有试剂均按照试剂盒内说明书推荐的条件存储。 【人轮状病毒抗原(RVAg)ELISA试剂盒现货】

8.检查不稳定或者变质的试剂溶液（比如沉淀或变色）。有些试剂，比如标准品稀释液或者检测稀释液，可能在设计时就含有沉淀物。所有试剂在滴入酶标板之前，必需充分混匀。而由于沉淀物的特性，在加入酶标板之前，必需不停搅拌。

9.保证充分的孵育时间和温度。

10.切勿使用不同生产批号的试剂取代现有试剂或混合现有试剂。

11.在混合或溶解蛋白溶液时，避免泡沫产生。

12.在实验开始之前，安排好实验流程。在实验开始之前，清洁工作台。

13.若有问题，应与我公司或代理商的技术支持联系

【人轮状病毒抗原(RVAg)ELISA试剂盒现货】四、客户须知

齐一生物销售：021－6034 8496；181214 53965；173021 04490

1.在收集标本前都必须有一个完整的计划，必须清楚要检测的成份是否足够稳定。我们提倡新鲜标本尽早检测，对收集后当天就进行检测的标本，及时储存在4℃备用，如有特殊原因需要周期收集标本，请造模取材后，将标本及时分装后放在-20℃或-70℃条件下保存。因冰室与室温存在一定温差，蛋白极易降解，直接影响实验质量，所以避免反复冻融。部分标本也可加入适当防腐剂，激素类标本需添加抑肽酶。 

2.试剂盒代测标本的客户取材前须向我司销售人员索要说明书，具体操作注意事项请与我司技术人员沟通。

3.液体类标本：标本必须为液体，不含沉淀。包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清、组织匀浆等。

4.购买前请提前告诉我们：您样本的种属、样本数量、待测指标及其它特殊要求。

【人轮状病毒抗原(RVAg)ELISA试剂盒现货】五、代测数据详细内容

1、ELISA标准曲线；

2、实验具体详细步骤；

3、完整的实验报告（试剂耗材，实验方法，实验结果及结果说明）；

4、实验进行阶段，我公司客服人员会及时向您汇报实验进程。

762431     PAXgene Blood RNA MDx Kit (4)               CNY                             

203745     QIAGEN Fast Cycling PCR Kit (1000)        For 1000 x 20 µl reactions: 10 x 1 ml Fast Cycling Master Mix (2x), RNase-free water, 10 x CoralLoad Fast Cycling Dye, 5 x Q-Solution       CNY                             

203609     HotStarTaq Plus DNA Polymerase (25000)       100 x 250 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 100 x 2.0ml Q-Solution, 25 mM MgCl2         CNY                             

201205     Taq DNA Polymerase (1000)    "4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."         CNY                             

180850     GeneRead qPCR FAST SYBR Green/ROX MM (2)               CNY                             

159300     QIAcard FTA Purification Reagent (500ml)                CNY                             

158928     Lytic Enzyme Solution (650 µl)          CNY                             

9240549  Imbalance PCB, Eppendorf, AP196/98               CNY                             

9240547  Motor, 230V, Eppendorf, AP196/98           CNY                             

9240528  Cable, Motor, Axis #5, TR, AP98                CNY                             

9240047  Thermostate unit, 120V, 1.04          CNY                             

9020029  Cable RC-3034, RGQ                  CNY                             

9020017  Oil for PM, EZ1                   CNY                             

9020016  Cable harness, VacuumStation PCB, CX1          CNY                             

9019938  Opto switch, P-axis, 1 ch. , Cx2                  CNY                             

9019486  HEPA filter, replacement cartridge, Cxx           CNY                              【人轮状病毒抗原(RVAg)ELISA试剂盒现货】

9238861  BR 8000, Premium Agreement        The Premium Cover Agreement 8000 provides full service coverage for the BioRobot 8000. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).   CNY                             

9238855  BR MDx, Basic Agreement      The Basic Cover Agreement MDx provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                            

9238850  EZ1, Basic Agreement     The Basic Cover Agreement EZ1 provides full service coverage for the BioRobot EZ1. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.      CNY                             

9241226  Barcode Reader, EZ1a              CNY                             

9238812  Rectangular carrier, undrilled          CNY                             

9019467  PLATE GLASS SHORT SET GS              CNY                             

9019465  Pads, D=10mm, white (85), Cxx                CNY                             

9019459  Transformer (115V), HS            CNY                             

9233429  Valve, magnet, dispenser, BR8000           CNY                             

9233334  Solenoid Valve, 5-fold                CNY                             

9237332  Install, BR plus 2 integr. w. scheduling     Installation ａnd training, BioRobot System including two approved integration devices with scheduling   CNY                             

9237167  Bottom Air Filter               CNY                             

9232994  Board, LED, BR8000                  CNY                             

9237158  RTD Adapter Board XYP           CNY                             

9237152  Power Inlet                CNY                             

9237108  Protocol Dev./Opt., on-site (4-days)         The QIAGEN BioRobot Systems Protocol Development/Optimization On-Site Visit includes all travel ａnd labor charges for a site visit up to 4 days by a QIAGEN Instrumentation Service Specialist to write and/or opimize protocols for the BioRobot System. Parts, consumable chemistries, ａnd accessories not included. Custom protocols are the sole responsibility of the customer ａnd neither warrantied nor guaranteed.          CNY                              【人轮状病毒抗原(RVAg)ELISA试剂盒现货】

9237098  Upgrade QIAsoft, on-site                   CNY                             

OPCM       少突胶质前体细胞培养基  Oligodendrocyte Precursor Cell Medium          500 ml                                 

OsM 球状少突细胞培养基  Oligosphere Medium        500 ml                                 

KM   角质细胞培养基  Keratinocyte Medium      500 ml                                 

KM-d         角质细胞培养基（确定）          Keratinocyte Medium-defined         500 ml                                 

FM-sf        成纤维细胞培养基         Fibroblast Medium-serum free        500 ml                                 

TEpiCM    扁桃体上皮细胞培养基         Tonsil Epithelial Cell Medium   500 ml                                 

OKM         口腔角质细胞培养基  Oral Keratinocyte Medium      500 ml                                  【人轮状病毒抗原(RVAg)ELISA试剂盒现货】

CoEpiCM  结肠上皮细胞培养基     Colonic Epithelial Cell Medium         500 ml                                 

BEpiCM    支气管上皮细胞培养基       Bronchial Epithelial Cell Medium     500 ml                                 

SAEpiCM  气管上皮细胞培养基  Small Airway Epithelial Cell Medium        500 ml                                 

EpiCM-2   上皮细胞培养基-2        Epithelial Cell Medium-2 500 ml                                 

UCM         尿道上皮细胞培养基  Urothelial Cell Medium    500 ml                                 

PEpiCM    前列腺上皮细胞培养基       Prostate Epithelial Cell Medium       500 ml                                 

STEMium 人胚胎干细胞无血清培养基         Human Pluripotent Stem Cell Growth Medium       500 ml                                 

STEMium-XF     无异种人胚胎干细胞生长培养基         Xeno-free Human Pluripotent Stem Cell Growth Medium       500 ml                                 

MEF-cm   小鼠胚胎成纤维细胞培养基         Mouse Embryonic Fibroblasts-Conditioned Medium        100 ml                                

bFGF-std MEF-cm     碱性成纤维细胞生长因子刺激的小鼠胚胎成纤维细胞条件培养基         bFGF-Stimulated Mouse Embryonic Fibroblasts Conditioned Medium     100ml                                  

CEpiCM    角膜上皮细胞培养基  Corneal Epithelial Cell Medium        500 ml                                 

OEpiCM    卵巢上皮细胞培养基  Ovarian Epithelial Cell Medium        500 ml                                 

MSCM-sf  间充质干细胞培养基  Mesenchymal Stem Cell Medium-serum free 500 ml                                 

MEpiCM   乳腺上皮细胞培养基  Mammary Epithelial Cell Medium   500 ml                                 

KM-acf     角质细胞培养基  Keratinocyte Medium-animal component free        500 ml                                 

FM-acf      成纤维细胞培养基       Fibroblast Medium-animal component free    500 ml                                  【人轮状病毒抗原(RVAg)ELISA试剂盒现货】

STEMium-ACF  人类多能干细胞培养基-无动物组分   STEMium® Human Pluripotent Stem Cell Growth Medium-animal component free          500ml                                  

MSCM-acf         间充质干细胞培养基     Mesenchymal Stem Cell Medium-animal component free        500 ml                            

MEM        MEM        MEM with Earle＇s Salts ａnd L-Glutamine        500 ml                                 

MEM       MEM       MEM with Hank＇s Salts ａnd L-Glutamine         500 ml                                 

MEM       MEM       MEM with Eagles＇s Salts ａnd Glutamine         500 ml                                 

M199E  M199E  Medium 199 with Earle＇s Salts ａnd L-Glutamine    500 ml                                 

M199H           M199H           Medium 199 with Hanks Salts ａnd L-Glutamine     500 ml                                 

DMEM    DMEM    DMEM with L-Glutamine ａnd Sodium Pyruvate     500 ml                                 

EpiCM       上皮细胞培养基  Epithelial Cell Medium     500 ml                                 

EpiCM-a   动物上皮细胞培养基  Epithelial Cell Medium-animal          500 ml                                 

MCM        肾系膜细胞培养基       Mesangial Cell Medium   500 ml                                 

ObM         成骨细胞培养基  Osteoblast Medium 500 ml                                 

CM   软骨细胞培养基     Chondrocyte Medium      500 ml                                 

SM   滑膜细胞培养基  Synoviocyte Medium        500 ml                                 

NPCM       髓核细胞培养基  Nucleus Pulposus Cell Medium         500 ml                                 

HM  肝细胞培养基       Hepatocyte Medium        500 ml                                 

SteCM      星形细胞培养基  Stellate Cell Medium        500 ml                                 

CMM        心肌细胞培养基  Cardiac Myocyte Medium        500 ml                                 

TMCM      小梁网细胞培养基         "Trabecular Meshwork Cell Medium

"       500 ml                                 

TM   滋养层细胞培养基       Trophoblast Medium        500 ml                                 

AdM          脂肪细胞培养基     Adipocyte Medium  500ml                                  

PAM 前脂肪细胞培养基       Preadipocyte Medium     500 ml                                 

PADM       脂肪细胞诱导分化培养基     Preadipocyte Differentiation Medium     500 ml                                  【人血管紧张素原(aGT)ELISA试剂盒厂家】

MSCM      间充质干细胞培养基  Mesenchymal Stem Cell Medium    500 ml                                 

MODM-500      间充质干细胞-成骨细胞分化培养基 Mesenchymal Stem Cell Oseogenic Differentiation Medium  500 ml                                 

MODM-250               Mesenchymal Stem Cell Oseogenic Differentiation Medium  250 ml                                 

MADM-500      间充质干细胞-脂肪细胞分化培养基   Mesenchymal Stem Cell Adipogenic Differentiation Medium 500 ml                                 

MADM-250               Mesenchymal Stem Cell Adipogenic Differentiation Medium 250 ml                                 

MCDM     间充质干细胞-软骨分化培养基   Mesenchymal Stem Cell Chondrogenic Differentiation Medium     500ml                                  

SMCM-sf  平滑肌细胞培养基         Smooth Muscle Cell Medium- serum free        500 ml                                 

NPrCM     神经前体细胞培养基     Neural Precursor Cell Medium         500 ml                                 

NM  神经细胞培养基  Neuronal Medium   500 ml