【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【人胸肾表达趋化因子(BRAK/CXCL14)ELISA试剂盒厂家】样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【人胸肾表达趋化因子(BRAK/CXCL14)ELISA试剂盒厂家】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

【人胸肾表达趋化因子(BRAK/CXCL14)ELISA试剂盒厂家】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【人胸肾表达趋化因子(BRAK/CXCL14)ELISA试剂盒厂家】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

870001    KRAS RGQ PCR Kit (24)             CNY                           

805965    REPLI-g Service,  HT (500 µg)          CNY                           

800443    cDNA Library, Picked Clones              CNY                           

290075    mericon S. aureus Kit (96)           CNY                           

9012187  Zahnstange spez. Nadelantrieb P1xxx          CNY                            【人胸肾表达趋化因子(BRAK/CXCL14)ELISA试剂盒厂家】

9012032  Base plate, Z motor, handler, BR8000           CNY                           

9001673  PyroMark Q96 MD Instrument     For performing pyrosequncing reaction on the Q96 MD     CNY                           

9001659  Rotor-Gene Q 5plex HRM (w/o PC) PrioPLUS    Rotor-Gene Q 5plex HRM (w/o PC) PrioPLUS includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 3 years with shipment to Repair Center Service ａnd response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 3 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.      CNY                           

9001657  Rotor-Gene Q 5plex HRM (w/o PC) System       Rotor-Gene Q 5plex HRM (w/o PC) System includes the Rotor-Gene system ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It covers the handling of the Rotorgene-Q systems hardware ａnd trains the user on operation of the Rotorgene software. All travel ａnd labor cost included for Installation ａnd service documented on a GMP/GLP-compliant Field Service document.    CNY                           

950233    Binding Reagent (VXB) (350 ml)          CNY                           

37571     Qproteome O-Glycan Glycoprotein Kit        CNY                           

9015330  Sensor, photo, EE-SX670A          CNY                           

9015322  Belt, M-motor, M48              CNY                           

133026    NeXtalStock Calcium chloride  (200)         CNY                           

133025    NeXtalStock Calcium acetate  (200)           CNY                           

9001630  Rotor-Gene Q 2plex HRM System        CNY                           

249900    QuantiTect Primer Assay (200)     For 200 x 50 µl reactions: lyophilized mix of forward ａnd reverse primers for real-time analysis with SYBR Green detection.    CNY                           

133006    NeXtalStock MES sod. salt pH 6.5 (200)            CNY                           

9011965  Tip Adapter 300 µl BR8000          CNY                           

204554    QuantiFast Probe RT-PCR+ROXvial Kit(400)             CNY                           

133064    NeXtalStock Magnesium sulfate 6H2O (200)              CNY                            【人胸肾表达趋化因子(BRAK/CXCL14)ELISA试剂盒厂家】

870101    EGFR RGQ PCR Kit (24)             CNY                           

290023    mericon L. monocytogenes Kit (24)            CNY                           

204443    QuantiTect Probe RT-PCR Kit (200)     For 200 x 50 ul reactions: 3 x 1.7 ml QuantiTect Probe RT-PCR Master Mix,* 100 ul QuantiTect RT Mix, 2 x 2.0 ml RNase-free water       CNY                           

133052    NeXtalStock CTAB (200)             CNY                           

9018397  Transport Box, QIAxcel        CNY                           

9001650  Rotor-Gene Q 5plex HRM System        CNY                           

37612     Qproteome Mitochondria Isolation Kit          CNY                           

9015333  O-rings, pressure sensor fixing, M48 (2)            CNY                           

133032    NeXtalStock Citric acid  (200)           CNY                           

133023    NeXtalStock Cadmium chloride  (200)              CNY                           

9001638  Rotor-Gene Q 2plex HRM (w/o PC) Priority       Rotor-Gene Q 2plex HRM (w/o PC) Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.      CNY                           

9001611  QIAgility HEPA / UV (incl.Laptop)        CNY                           

 QY-021029     CD49(非常晚期抗原)抗体   Anti-CD49/VLA(very late Anti-gens)                               

 QY-021030     CD5抗体       Anti-CD5                                   【人胸肾表达趋化因子(BRAK/CXCL14)ELISA试剂盒厂家】

 QY-021031     细胞间粘附分子-1抗体       Anti-CD54/ICAM-1(Intercellular dhesion molecule 1)                            

 QY-021032     细胞间粘附分子-2抗体       Anti-CD102/ICAM-2（intercellular adhesion molecules-2）                                 

 QY-021033     细胞间粘附分子-3抗体       Anti-CD50/ICAM-3（intercellular adhesion molecules-3）                            

 QY-021034     衰变加速因子CD55抗体    Anti-CD55/DAF                               

 QY-021035     反应性溶血膜抑制蛋白抗体       Anti-CD59/potectin                                  

 QY-021036     神经细胞粘附分子1抗体（Elisa IP IHC 用）  Anti-CD56/NCAM1(Neural Cell Adhesion Molecule 1)                                  

 QY-021037     神经细胞粘附分子1抗体    Anti-CD56/NCAM1(Neural Cell Adhesion Molecule 1)                                  

 QY-021038     神经细胞粘附分子配体1抗体  Anti-CD171/NCAM-L1（Neural cell adhesion molecule ligand 1）                               

 QY-021039     CD57抗体     Anti-CD57                                

 QY-021040     CD68抗体（用于大鼠、小鼠）  Anti-CD68（to Mouse, rat）                                  

 QY-021041     CD68抗体（人、牛）  Anti-CD68（to human,bocine）                              

 QY-021042     刺激分子B7-1蛋白抗体      Anti-CD80/B7-1                               

 QY-021043     转铁蛋白受体抗体       Anti-CD71/TFR(Transferrin Receptor)                                  

 QY-021044     胞浆-5´-核苷酸酶-Ⅱ抗体   Anti-CD73(5´-nucleotidase cytosolicⅡ)                                

 QY-021045     免疫球蛋白结合蛋白-1抗体       Anti-CD79A/IGBP-1(Immunoglobuin binding protein-1)                               

 QY-021046     肿瘤转移抑制蛋白1抗体    Anti-CD82/KAI1                                【人胸肾表达趋化因子(BRAK/CXCL14)ELISA试剂盒厂家】

 QY-021047     鼠抗人CD8单克隆抗体      Anti-CD8（mouse monoclonal antibody）                             

 QY-021048     CD86抗体     Anti-CD86/B7-2                               

 QY-021049     周期素依赖激酶1抗体       Anti-Cdc2/P34CDC2/CDK1(Cell division control protein 2)                                  

 QY-021050     细胞毒性受体NK-p46抗体        Anti-CD335/NKp46/NCR1                               

 QY-021051     细胞毒性受体NK-p44抗体        Anti-CD336/NKp44/NCR2(Natural killer cell p44-related protein)                                 

 QY-021052     细胞毒性受体NK-p30抗体        Anti-CD337/NKp30/NCR3(Natural killer cell p30-related protein)                                 

 QY-021053     细胞分裂周期蛋白6抗体    Anti-Cdc6（Cell Division Cycle protein 6）                            

 QY-021054     抗Jagged1/CD339抗体        Anti-CD339/Jagged1                                

 QY-021055     细胞周期调控因子34   Anti-Cdc34（Cell Division Cycle protein 34）                               

 QY-021056     周期素依赖性激酶1抗体    Anti-CDK1 (Cyclin-Dependent Kinase 1)                                

 QY-021057     周期素依赖性激酶2抗体    Anti-CDK2 (Cyclin-Dependent Kinase 2)                                

 QY-021058     周期素依赖性激酶3抗体    Anti-CDK3 (cyclin-dependent kinase 3)                                  

 QY-021059     周期素依赖性激酶4抗体    Anti-CDK4 (cyclin-dependent kinase 4)