【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【人可溶性CD86(B7-2/sCD86)ELISA试剂盒厂家】样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【人可溶性CD86(B7-2/sCD86)ELISA试剂盒厂家】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

【人可溶性CD86(B7-2/sCD86)ELISA试剂盒厂家】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【人可溶性CD86(B7-2/sCD86)ELISA试剂盒厂家】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

QY-x638  PC-3前列腺癌细胞                                        

QY-x639  BEL-7402肝细胞癌细胞                                        

QY-x640  Tsu-Pr1非雄激素依赖型前列腺癌                                       

QY-x641  HCC-9204肝癌细胞                                       

QY-x642  HOS骨肉瘤细胞                                     

QY-x643  Hep3B肝癌细胞                                      

QY-x644  MG-63成骨肉瘤细胞                                     

QY-x645  Hep G2肝癌细胞                                     

QY-x646  Saos-2骨肉瘤细胞                                         

QY-x647  SMMC-7721肝癌细胞                                     【人可溶性CD86(B7-2/sCD86)ELISA试剂盒厂家】

QY-x648  U-2 OS骨肉瘤细胞                                        

QY-x649  PAN-1胰腺导管上皮癌细胞                                         

QY-x650  OS-732骨肉瘤细胞（瘤株）                                        

QY-x651  Panc 10.05胰腺腺癌细胞                                      

QY-x652  A-204横纹肌肉瘤细胞                                   

QY-x653  SW1990胰腺腺癌细胞                                   

QY-x654  HT1080纤维肉瘤细胞                                    

QY-x655  SK-RC-42肾癌细胞                                        

QY-x656  A375皮肤黑色素瘤细胞                                        

QY-x657  RCC-krause肾癌细胞                                     

QY-x658  A875黑色素瘤细胞                                        

QY-x659  Ketr-3肾癌（瘤株）                                      

QY-x660  SK-HEL-1皮肤黑色素瘤细胞                                        

QY-x661  SW 13肾上腺皮质瘤细胞                                      

QY-x662  A431皮肤基底细胞癌细胞                                    

QY-x663  96-C低转移肺癌细胞                                      【人可溶性CD86(B7-2/sCD86)ELISA试剂盒厂家】

QY-x664  CEM白血病细胞                                     

QY-x665  95-D高转移肺癌细胞                                     

QY-x666  K562慢性髓原白血病细胞                                    

QY-x667  973肺腺癌细胞                                       

QY-x668  HPB-ALLT细胞白血病细胞                                   

QY-x669  A2肺腺癌细胞                                        

QY-x670  HL-60白血病细胞                                   

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9017625  Motor, y-drive (24V), BR8RD              CNY                           

9020254  LED, LightStrip (white), Qsym            CNY                           

9017354  Holder, VacTrap, BR8           CNY                           

9020201  PCB, lrge breakout, 12 core to rev8, CC2           CNY                           

9020199  PCB, Z plate breakout,8 ch., 8 core, QX1           CNY                           

9020193  PCB, vac pump, QXT           CNY                           

9239523  Fitting,1/16MNPT,1/8, forWasteBottle(5)            CNY                           

9239536  Assy,Terminated,RedLED,SDS (2)              CNY                           

9239529  Fuse&Holder,1Amp,Fblo,PicoSmd(5)          CNY                           

9020159  Mask, protection - chamber bottom, RG3           CNY                           

9020145  QIAplex MDD HPV Genotyping LQ Software            CNY                           

9020114  USB cable, QGX           CNY                           

9020102  Packaging, TLyser2              CNY                            【人可溶性CD86(B7-2/sCD86)ELISA试剂盒厂家】

9020100  Shipping Lock Screw, TLyser 2           CNY                           

9020094  Left Ratchet Holder, TLyser 2              CNY                           

9020090  Motor 24V DC, TLyser 2             CNY                           

9239518  Tubing. Peek, 1/6OD, 010ID, 10 (qty 5)             CNY                           

9239492  Hyd Arm Optic Switch          CNY                           

9239449  3A, 250V Fast Acting Fuse           CNY                           

9232867  Sensor, pressure, 1600-1mbar             CNY                           

9236775  Sealing ring, auto vac, SBS top, BR3000             CNY                           

9236509  BR 8000, PM Service    The Overhaul covers all travel ａnd labor costs plus all necessary parts to perform a Preventative Maintenance,. The instrument will also be thoroughly checked ａnd inspected to determine if any additional parts are required to get the instrument running to its optimal performance. There will be an additional charge for any such parts if any are needed. CNY                           

9239360  Z-Axis Coupling, EZ1           CNY                           

9236478  BR 9604, Full Agreement       The Full Agreement 9604, 1 PM provides full service coverage for the BioRobot 9604. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).       CNY                           

9236476  #Agreement, 9604, 2PM, 1 yr       An agreement for preventive maintenance provides coverage for travel, labor, ａnd parts, plus the cost of consumables required for two maintenance visits during 1 year. Work carried out during the preventive maintenance visit is fully documented in accordance with GMP/GLP directives.    CNY                           

9232726  Block, 2-way, v1.02             CNY                           

9239540  Cable Assy Extender, 2 wire, 22AWG, 12in          CNY                           

9239396  Upgr. LiquiChip Analyzer (RUO)          CNY                           

9239496  EX XY Motor Driver w/Encoder          CNY                            【人可溶性CD86(B7-2/sCD86)ELISA试剂盒厂家】

9239494  Sheath Fluid Tubing w/DeBubbler        CNY