【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【人正常T细胞表达和分泌因子(RANTES/CCL5)ELISA试剂盒厂家】样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【人正常T细胞表达和分泌因子(RANTES/CCL5)ELISA试剂盒厂家】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

【人正常T细胞表达和分泌因子(RANTES/CCL5)ELISA试剂盒厂家】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【人正常T细胞表达和分泌因子(RANTES/CCL5)ELISA试剂盒厂家】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

QY-x1723       狗肾细胞       MDCK           狗肾脏    DMEM＋10％NBCS           

QY-x1724       人胚肾细胞（野生型）       293T-rex-wild-M2         人胚肾    DMEM＋10％NBCS           

QY-x1725       人胚肾细胞（31位点突变）      293T-rex-S 31N            人胚肾    DMEM＋10％NBCS           

QY-x1726       人胃腺癌细胞       MCG803                            

QY-x1727       人胃腺癌细胞       SCG-7901                                 

QY-x1728       人胃癌细胞(未分化)     HGC-27                             

QY-x1729       人结肠癌细胞       Lovo                                  

QY-x1730       人结肠癌细胞       SW480                                【人正常T细胞表达和分泌因子(RANTES/CCL5)ELISA试剂盒厂家】

QY-x1731       人肝细胞癌细胞    BEL-7402                                  

QY-x1732       人肝细胞癌细胞    SMMC-7721                              

QY-x1733       人肝癌细胞   QGY- 7701                                

QY-x1734       人肝癌细胞    Hep-3b                               

QY-x1735       转染四环素正向调控表达基因7402细胞  BEL-7402-Tet-on                              

QY-x1736       转染绿色荧光蛋白和四环素正向调控表达基因7402细胞      7402-Tet-on-EGFP                            

QY-x1737       人胰腺癌细胞       Panc-1                               

QY-x1738       人胰腺癌细胞       SW1990                             

QY-x1739       人口腔上皮癌细胞       KB                              

QY-x1740       人口底癌细胞耐药株［长春新碱（VCR）诱导高表达（p-gp）］   KV                              

QY-x1741       人肺巨细胞癌细胞       HLAmP                              

QY-x1742       人肺腺癌细胞       GLC-82                              

QY-x1743       人肺腺癌细胞（转染TK基因的GLC-82细胞）      GLC-82-TK                               

QY-x1744       人鼻咽癌细胞（高分化）    CNE-1                               

QY-x1745       人鼻咽癌细胞       SUME-a                             

QY-x1746       人鼻咽癌细胞       HNE-2                                【人正常T细胞表达和分泌因子(RANTES/CCL5)ELISA试剂盒厂家】

QY-x1747       人慢性髓原白血病细胞       K562                                  

QY-x1748       人组织细胞淋巴瘤细胞       U937                                  

QY-x1749       人红白细胞白血病细胞Erythroleukemia    HEL                                  

QY-x1750       人急性T淋巴母细胞白血病细胞       MOLT-4                             

QY-x1751       人急性早幼粒白血病细胞（悬浮）    NB4                            

QY-x1752       人骨肉瘤细胞       SW1353                             

QY-x1753       人白血病细胞       Daudi                                 

QY-x1754       转染了tk基因的SW038-C2细胞       SW038-C2-tk                            

QY-x1755       人星形胶质细胞瘤细胞      SHC-44                              

9240716  Lead Screw Assy, Capper U\D, AP196/98           CNY                           

9240712  Muffler, Elevator Brake, AP196/98              CNY                           

5000-1416      Switch LRGSK61RCBE250, VTX2             CNY                           

6000-5023      LumiCheck Plate User Package (CD Manual)             CNY                           

6000-3102      Rapid Capture System & Manuals        CNY                           

12941     QIAGEN Plasmid Plus Midi Sample Kit (5)  QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega ａnd Giga) or up to 24 (Midi ａnd Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction. CNY                           

9240695  Needle Valve, Flow Control, AP196/98         CNY                           

9002141  careHPV test Shaker             CNY                           

273501    pigtype Trichinella Ab (1)             CNY                           

273803    pigtype Yersinia Ab (5)          CNY                           

280433    virotype BTV RT-PCR Kit (24)            CNY                           

281607    virotype SBV RT-PCR Kit (480)           CNY                           

800371    Shipping,Postage,Packaging (stand. mail)            CNY                           

9019497  ZLV MANUAL CAS1200              CNY                           

9020112  Accessory power lead (vac. station), QXT          CNY                           

9020578  Adapter, USB to Serial, DML 3000              CNY                            【人正常T细胞表达和分泌因子(RANTES/CCL5)ELISA试剂盒厂家】

9020587  Pulley, motor, DML 3000             CNY                           

9020595  Rubber Foot, 2" Dia x 1" Ht, DML 3000             CNY                           

9232722  Cooling block, 96-tube 0.2 ml       Holder for accommodating 96-well amplification plate (0.2 ml per well) on the worktable       CNY                           

9013265  Cable, 24V, power to cooling, 1900 mm              CNY                           

9013257  Tubing set , HSPS, 90/120,0        CNY                           

9013250  BGR Waste System              CNY                           

9013216  Screws, plastic, M4 (20)              CNY                           

9013202  Valve set, throttle, auto vac, BR8000            CNY                           

9011758  Reagent holder, 8-tube 1.5 ml       Holder for accommodating 8 microcentrifuge tubes (1.5 ml) on the worktable; must be used with the reagent holder tray   CNY                           

132625    NeXtal Stock TRIS. HCl (50)       1M Tris(hydroxymethyl)aminomethane HCl - 50mL (4 x 12,5 ml tube)     CNY                           

9241374  Magnet, door lock, bottom, EZ1a         CNY                           

9241373  Cable, key panel board, EZ1a        CNY                           

9240752  Downtube, Reagent #2-6, AP98           CNY                           

9240751  Belt, TR,Capper U/D & I/O, AP           CNY                           

9243546  QIAsymphony SP AS, PM Service       "

Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage" CNY