1.购买我公司任意ELISA试剂盒指标享受免费代测，并提供技术指导服务！

二、实验材料与试剂配制：

1. 仪器与材料：酶标仪（使用前预热30分钟），微量加液器、吸头、蒸馏水或去离子水，滤纸。

2. 缓冲液使用：加5ul  的缓冲液于50ul  的样品中，如果样品量不够或者不确定，缓冲液和样品的混合比例不要小于1:10即可混匀，静置1小时备用（如果标本是血清或者血浆，此步骤忽略）。

3. 洗液的配制：按1:100的比例配制洗液备用。

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人巨噬细胞来源的趋化因子(MDC/CCL22)ELISA试剂盒现货】三. 仔细阅读说明书

1.检查试剂盒标签上的有效期。如果超过有效期，请勿使用。

2. 按说明书确定所有试剂齐全（数量、体积）

3. 标本制备要规范，每份标本体积要按2-3个复孔以上的量制备（贮存），尽量分装做备份。

4.短期内无法实验者，注意低温保存

5.准备好所有实验额外所需物品，（比如移液器，试管，清洗器，酶标仪）

6. 按说明书将所用试剂平衡至室温，根据检测标本数量确定所需试剂的量

7.检查试剂盒内每种试剂的贮存条件，保证所有试剂均按照试剂盒内说明书推荐的条件存储。 【人巨噬细胞来源的趋化因子(MDC/CCL22)ELISA试剂盒现货】

8.检查不稳定或者变质的试剂溶液（比如沉淀或变色）。有些试剂，比如标准品稀释液或者检测稀释液，可能在设计时就含有沉淀物。所有试剂在滴入酶标板之前，必需充分混匀。而由于沉淀物的特性，在加入酶标板之前，必需不停搅拌。

9.保证充分的孵育时间和温度。

10.切勿使用不同生产批号的试剂取代现有试剂或混合现有试剂。

11.在混合或溶解蛋白溶液时，避免泡沫产生。

12.在实验开始之前，安排好实验流程。在实验开始之前，清洁工作台。

13.若有问题，应与我公司或代理商的技术支持联系

【人巨噬细胞来源的趋化因子(MDC/CCL22)ELISA试剂盒现货】四、客户须知

齐一生物销售：021－6034 8496；181214 53965；173021 04490

1.在收集标本前都必须有一个完整的计划，必须清楚要检测的成份是否足够稳定。我们提倡新鲜标本尽早检测，对收集后当天就进行检测的标本，及时储存在4℃备用，如有特殊原因需要周期收集标本，请造模取材后，将标本及时分装后放在-20℃或-70℃条件下保存。因冰室与室温存在一定温差，蛋白极易降解，直接影响实验质量，所以避免反复冻融。部分标本也可加入适当防腐剂，激素类标本需添加抑肽酶。 

2.试剂盒代测标本的客户取材前须向我司销售人员索要说明书，具体操作注意事项请与我司技术人员沟通。

3.液体类标本：标本必须为液体，不含沉淀。包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清、组织匀浆等。

4.购买前请提前告诉我们：您样本的种属、样本数量、待测指标及其它特殊要求。

【人巨噬细胞来源的趋化因子(MDC/CCL22)ELISA试剂盒现货】五、代测数据详细内容

1、ELISA标准曲线；

2、实验具体详细步骤；

3、完整的实验报告（试剂耗材，实验方法，实验结果及结果说明）；

4、实验进行阶段，我公司客服人员会及时向您汇报实验进程。

QY-x2086 HIH 3T3 小鼠胚胎成纤维细胞 1640+10%胎牛血清                                                

QY-x2087 L929 小鼠成纤维细胞 1640+10%胎牛血清                                                      

QY-x2088 HSC-T6 大鼠肝星状细胞 1640+10%胎牛血清                                                  

QY-x2089 L-02 人肝细胞 1640+10%胎牛血清                                                   

QY-x2090 SRA01/04 人晶体上皮细胞 MEM+非必需氨基酸＋10%胎牛血清                                                  

QY-x2091 CHO 中国仓鼠卵巢细胞（野生型） 1640+10%胎牛血清                                                       

QY-x2092 IEC-6 大鼠小肠隐窝上皮细胞 DMEM+10%胎牛血清                                                      

QY-x2093 P815 小鼠肥大细胞 1640+10%胎牛血清                                                 

QY-x2094 NRK-49F 大鼠正常肾成纤维细胞 1640+10%胎牛血清                                                    

QY-x2095 自建 正常成人皮肤成纤维细胞 DMEM+20%胎牛血清 原代培养                                                

QY-x2096 HELF 人胚肺成纤维细胞 1640+10%胎牛血清 5，6代                                                   

QY-x2097 293 人胚肾细胞 DMEM+10%胎牛血清                                                    

QY-x2098 293T 人胚肾T细胞 1640+10%胎牛血清                                                   【人巨噬细胞来源的趋化因子(MDC/CCL22)ELISA试剂盒现货】

QY-x2099 GES-1 人胃粘膜细胞 DMEM+10%胎牛血清                                                     

QY-x2100 3T3-L1 小鼠胚胎成纤维细胞（前脂肪） DMEM+10%胎牛血清                                                   

QY-x2101 Vero 猴肾细胞 1640+10%胎牛血清                                                  

QY-x2102 CHO-dhFr- 中国仓鼠卵巢细胞(二氢叶酸缺陷型） 1640+10%胎牛血清                                               

QY-x2103 V79 中国仓鼠肺细胞 1640+10%胎牛血清                                               

QY-x2104 PA317 小鼠胚胎成纤维细胞 1640+10%胎牛血清                                                    

QY-x2105 Cos-7 绿猴肾细胞 DMEM+10%胎牛血清                                                 

QY-x2106 MA104 非洲绿猴胚胎肾上皮细胞 DMEM+10%胎牛血清                                                       

QY-x2107 16HBE 人支气管上皮细胞 1640+10%胎牛血清                                                       

QY-x2108 Marc145 猴胚胎肾上皮细胞                                                      

QY-x2109 MDA-MB-435S 人乳腺导管癌细胞                                                     

QY-x2110 MDBK 瘤牛肾细胞                                                       

QY-x2111 MEG-01 人成巨核细胞白血病细胞                                                   

QY-x2112 MFC 小鼠胃癌细胞                                                      

QY-x2113 MG-63 人骨肉瘤细胞                                                 

QY-x2114 MLA144长臂猿淋巴瘤细胞                                                        【人巨噬细胞来源的趋化因子(MDC/CCL22)ELISA试剂盒现货】

QY-x2115 MouseM 小鼠肌肉细胞                                                       

QY-x2116 MouseS 小鼠皮肤成纤维样细胞                                               

QY-x2117 Hep G2人肝癌细胞系                                                

QY-x2118 Hep3B人肝癌细胞系                                                 

9013170  Tubing, syringe inlet 2, BR8000                 CNY                             

9011417  Klemmplättchen Varispan P7 (Alodine)             CNY                             

5050-1020EN   #Keyboard English Spare          CNY                             

9240731  Tubing, 3/8"OD , Blue, AP                   CNY                             

676091     ipsogen CBFB-MYH11 A Standards           CNY                             

9011341  Microplate Washer Module Plato 3                   CNY                             

9011203  Vacuum control unit, BR8000           CNY                             

673522     ipsogen JAK2 MutaQuant Kit (12) CE                 CNY                             

337839     qBiomarker SYBR Fluor Mastermix 25 ml                  CNY                             

337632     qBiomarker SYBR Green ROX Mastermix (12)                   CNY                             

9018907  36-Well Rotor            CNY                              【人巨噬细胞来源的趋化因子(MDC/CCL22)ELISA试剂盒现货】

9018783  CAT, Qsym                  CNY                             

9018694  EZ1 Adv. XL DNA Bacteria Card                 CNY                             

9018565  Door lock, mechanical, EZ1a            CNY                             

9013132  Elevating Platform            CNY                             

4509063  artus WNV LC RT-PCR Kit (24) CE              CNY                             

9001905  #QIAgility (w/o PC) System       QIAgility (w/o PC) System combines the QIAgility instrument ａnd installation. The Installation plus Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.    CNY                             

800383     Open Reading Frame Search            CNY                             

9019052  Notebook 2 XP          CNY                             

9020200  ZLV PCB, USB, 18Q            CNY                             

9020580  Cable, DB9 M/F (UL) 6ft, DML 3000                  CNY                             

9020588  Pulley, belt, DML 3000              CNY                             

9021016  Robot Stage, X Axis, VR, DCU           CNY                             

9013376  Maintenance Kit BR 8000 Research                  CNY                             

9013279  BGR Upgrade-Kit Scanner PK80                CNY                             

9013272  Peristaltic Pump, RCS1              CNY                             

9013190  Motor, rotor, buffer track, BR8000          CNY                              【人巨噬细胞来源的趋化因子(MDC/CCL22)ELISA试剂盒现货】

132772     NeXtal Stock Jeffamine M-600 (200)        100% v/v O-(2-Aminopropyl)-O＇-(2-methoxyethyl)polypropylene 500 - 200mL (20 x 10 ml tube)       CNY                             

132620     NeXtal Stock Tricine (50) 1M Tricine - 50mL (4 x 12,5 ml tube)        CNY                             

132560     NeXtal Stock Magnesium chloride (50)    2M Magnesium chloride hexahydrate - 50mL (4 x 12,5 ml tube)    CNY                            

800171     Easy-Read Tubes, w/o prep      "Sequencing of plasmid DNA ａnd PCR products

DNA quality control not included

No repetition of sequencing

Data collected to maximal read length" CNY                             

QY-x2083 PC-12 嗜铬细胞瘤 1640+10%胎牛血清+5%马血清 体外培养株                                                    

QY-x2084 [编辑本段]细胞库部分细胞系目录（遗传和正常细胞）                                                

QY-x2085 细胞名称 组织类型 培养条件                                                 

5000-1311        Capacitor 8UF, SKR1                  CNY                             

5000-1273        Door Damper Assembly, DML2                 CNY                             

5000-1258        Y Optical Sensor CMPLT, DML2                  CNY                             

5050-1074 V.1 CD Rom Manual - LumiCheck Plate          CNY                             

5050-1069 V.1 CD Rom Manual - Rotary Shaker I, 240V                   CNY                             

6000-5001        Costar Plate Lids                CNY                             

6000-3120        Plate Washer I - WasteReserv.CapAssembly             CNY                             

5000-1420        Driveplate Asmly 099A, VTX2           CNY                             

5127-1220        hc2 Sample Conversion Kit                CNY                             

9243701  careHPV, Training             CNY                              【人巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA试剂盒厂家】

EA11423  EASYartus BKV RG_I                  CNY                             

800190     Sequencing in a Box Service              CNY                             

800110     Single Read, w/ prep        "Bacterial clone cultivation ａnd template purification or PCR product purification, including DNA quality control

Repetition of sequencing, if necessary

Data collected to maximal read length" CNY                             

132538     NeXtal Stock Dioxane (50)        100% v/v Dioxane - 50mL (5 x 10 ml tube)       CNY                             

338162     RT2 PCR Array Loading Reservoir             CNY                             

338114     Mouse XpressRef Universal Total RNA              CNY                             

203743     QIAGEN Fast Cycling PCR Kit (200)  For 200 x 20 µl reactions: 2 x 1 ml Fast Cycling Master Mix, 2x (containing dNTPs, patent pending buffer composition), RNase-free water, 10 x CoralLoad Fast Cycling Dye, 5 x Q-Solution CNY                             

180820     GeneRead qPCR SYBR Green MM (2)               CNY                             

159320     Desicant (1000)                 CNY                             

159142     QIAsafe DNA Blood 48-Well Plates (10)            CNY                             

158822     Gentra Puregene Buccal Cell Kit (10)                CNY                             

158689     Gentra Puregene Tissue Kit (33 g)            CNY                             

678691     ipsogen MLL-AF9 type A e10e6 Standards                CNY                             

32943       cis-Repressed pQE-Kan Vector Set            CNY                             

32506       EasyXpress Protein Synthesis Maxi Kit     For reactions up to 4000 ul: 4 x 350 ul E. coli extract, reaction buffer, RNase-free water, ａnd positive-control DNA     CNY                             

30930       Glutathione HiCap Matrix (100 ml)          CNY                             【人巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA试剂盒厂家】

30725       #Ni-NTA Superflow Cartridges (100x1 ml)                 CNY                             

30210       Ni-NTA Agarose (25 ml)   25 ml nickel-charged resin (max. pressure: 2.8 psi)          CNY                             

28183       QIAquick 96 PCR Purification Kit (24)       For purification of 24 x 96 PCR reactions: 24 QIAquick 96 Plates, Buffers, Collection Microtubes (1.2 ml), Caps        CNY                             

9011608  BGR Kamm für Stirrer PK80 neu                CNY                             

9013178  Gripper set, BR8000 v 1.00               CNY