检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供*优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【人单核细胞趋化蛋白2(MCP-2/CCL8)ELISA试剂盒现货】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人单核细胞趋化蛋白2(MCP-2/CCL8)ELISA试剂盒现货】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在、第二孔中分别加标准品100μl,然后在、第二孔中加标准品稀释液50μl,混匀；然后从孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【人单核细胞趋化蛋白2(MCP-2/CCL8)ELISA试剂盒现货】

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品*终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果. 【人单核细胞趋化蛋白2(MCP-2/CCL8)ELISA试剂盒现货】

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请*后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

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QY-x2219 BABL/C2 BABL/C小鼠胚胎细胞                                                   

QY-x2220 BABL/3T3 BABL/C小鼠胚胎成纤维细胞                                                      

QY-x2221 BALL-1 人B淋巴细胞急性白血病细胞                                                      

QY-x2222 Bcap-37 人乳腺癌细胞                                               

QY-x2223 BHK 叙利亚仓鼠肾细胞                                                      

QY-x2224 BHK-21 叙利亚仓鼠肾细胞                                                

QY-x2225 BIU-87 人浅表性膀胱癌细胞                                                      

QY-x2226 BRL-3A 大鼠肝细胞                                                      【人单核细胞趋化蛋白2(MCP-2/CCL8)ELISA试剂盒现货】

QY-x2227 BT-325 人脑多形性胶质母细胞瘤细胞                                                      

QY-x2228 BV-2 小鼠小脑胶质瘤细胞                                                 

QY-x2229 BxPC3 人原位胰腺癌细胞                                                  

QY-x2230 C127 小鼠乳腺癌细胞                                                

QY-x2231 C6/36 白纹伊蚊细胞                                                    

QY-x2232 C6-121 大鼠神经胶质瘤细胞                                                      

QY-x2233 C6-1752 大鼠神经胶质瘤细胞                                                   

QY-x2234 C6-64 大鼠神经胶质瘤细胞                                               

QY-x2235 C6-70 大鼠神经胶质瘤细胞                                               

QY-x2236 C6-D2B 大鼠神经胶质瘤细胞                                                     

QY-x2237 C8166-CD4 人T细胞性白血病细胞                                                   

QY-x2238 CA 人肝癌细胞                                                    

QY-x2239 Caco-2 人结肠腺癌细胞                                                      

QY-x2240 CAM191 EBV-转化人淋巴细胞                                                     

QY-x2241 CAM200 EBV-转化人淋巴细胞                                                     

QY-x2242 CatK2 家猫肾上皮细胞                                               【人单核细胞趋化蛋白2(MCP-2/CCL8)ELISA试剂盒现货】

QY-x2243 CatL1 家猫肺成纤维样细胞                                               

QY-x2244 CatL2 家猫肺成纤维样细胞                                               

QY-x2245 CatS2 家猫皮肤成纤维样细胞                                                   

QY-x2246 CFPAC-1 人胰腺癌细胞                                               

QY-x2247 CHL 中国仓鼠肺细胞                                                  

QY-x2248 CHL-Don 中国仓鼠肺成纤维样细胞                                                  

QY-x2249 CHO 中国仓鼠卵巢细胞                                                      

QY-x2250 CHO/dhfr- 中国仓鼠卵巢细胞,                                                   

9238884  Stepper motor, turntable, BS96                CNY                             

9238866  BR MDx, Premium Agreement        The Premium Cover Agreement MDx provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).   CNY                             

9238857  Basic Cover Agreement Plato3, 1yr          The Basic Cover Agreement Plato3 provides full service coverage for the Plato3 platform. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9238838  #Twister I, Basic Agreement, no PM        The Basic Cover Agreement BR Twister I provides full service coverage for the BioRobot Twister I. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Basic Cover Agreement, the response time is 5 working days.     CNY                             

9241220  QIAxcel, Installation         "The Installation, QIAxcel is an on-site service, conducted by a QIAGEN Service Specialist. During the installation, the QIAxcel hardware is set up according to the manufacturer’s instructions.

This installation covers the handling of the QIAxcel systems hardware ａnd familiarize with the basic instrument ａnd software functions of the BioCalculator software. All travel ａnd labor costs ａnd well as material for the demonstration run are included. The service is documented in accordance with GMP/GLP requirements."    CNY                             

9241195  Cable, RS232, Belkin F2N209-06-T, 1.8m                   CNY                             

9241186  Sensor, interlock2, with cable, QX            CNY                             

9238831  BR 8000, Basic Agreement      The Basic Cover Agreement 8000 provides full service coverage for the BioRobot 8000. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                            

9238822  Rect. carrier for 1 tube 100 ml                  CNY                             

9237194  Reservoir RapidPlate                 CNY                             

9237171  Heater Block XYP              CNY                             

9233168  Mixer Board              CNY                             

9237154  Drive Mechanism XYP               CNY                             

9237110  Upgrade App., QIAamp 96 DNA Blood    On-site application Upgrade for standard QIAGEN QIAamp 96 DNA Blood including kit     CNY                             

9237095  Training, HiLight On-site  On-site Training 1 day HiLight CNY                              【人单核细胞趋化蛋白2(MCP-2/CCL8)ELISA试剂盒现货】

9237084  Installation, BR plus 3 integrations           CNY                             

9236414  Plate Stacker TW II           CNY                             

9232973  Level Detector Board P7-8 channels                 CNY                             

9232954  LSW Board (Level sensor waste)                CNY                             

9232921  Comparator board 1.02 w/clot detection                 CNY                             

9236337  Digital Board             CNY                             

9236155  Tool, calibration, syringe, RapidPlate                CNY                             

9235702  Cables, Shaker, RCS1                  CNY                             

9235626  Lead Screw Nut, MP Holder LH, Shaker8000           CNY                             

9235297  Plunger tip, syringe 250µl                  CNY                             

9235122  Fuse T1A, v1.00                 CNY                             

9235121  Fuse T2,5A, v1.00              CNY                             

9235119  Fuse F3.15A, v1.00            CNY                             

9238667  Front panel                CNY                             

9238646  Diode BY 500-600, 600 V 5 A            CNY                             

9238621  Power supply, motorized lid locks             CNY                              【人单核细胞趋化蛋白2(MCP-2/CCL8)ELISA试剂盒现货】