价格详细资料：试剂盒价格电议，齐一生物销售：021－6034 8496；181214 53965；173021 04490,齐一生物科技（上海）有限公司提供ELISA试剂盒受到了广大研究所.中科院科研单位一致肯定和认同。齐一生物大品牌保证，倾力为国内外科研院校实验室提供*优质产品。

【人游离三碘甲状腺原氨酸(Free-T3)ELISA试剂盒厂家】注意事项

1. 当混合蛋白溶液时应尽量轻缓，避免起泡。

2. 洗涤过程非常重要，不充分的洗涤易造成假阳性。

3. 一次加样时间控制在5分钟内，如标本数量多，推荐使用排枪加样。

4. 请每次测定的同时做标准曲线，做复孔。

5. 如标本中待测物质含量过高，请先稀释后再测定，计算时请*后乘以稀释倍数。

6. 在配制标准品、检测溶液工作液时，请以相应的稀释液配制，不能混淆。

7. 底物请避光保存。

8. 不要用其它生产厂家的试剂替换试剂盒中的试剂。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【人游离三碘甲状腺原氨酸(Free-T3)ELISA试剂盒厂家】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【人游离三碘甲状腺原氨酸(Free-T3)ELISA试剂盒厂家】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【人游离三碘甲状腺原氨酸(Free-T3)ELISA试剂盒厂家】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

QYD610   猪副嗜血杆菌- 抗体检测ELISA    96孔/盒                                      

QYD611   猪轮状病毒- 抗体检测ELISA        96孔/盒                                      

QYD612   猪轮状病毒- 抗原检测ELISA        96孔/盒                                      

QYD613   反刍动物弓形虫抗体检测ELISA   96孔/盒                                      

QYD614   非洲猪瘟抗体检测试剂盒     96孔/盒                                      

QYD615   鲤鱼卵黄蛋白原试剂盒         96孔/盒                                      

QYD616   虹鳟鱼卵黄蛋白原试剂盒     96孔/盒                                      

QYD617   鲑科鱼卵黄蛋白原试剂盒     96孔/盒                                      

QYD618   斑马鱼卵黄蛋白原试剂盒     96孔/盒                                      

QYD619   青鳉鱼卵黄蛋白原试剂盒     96孔/盒                                      

QYD620   黑鱼卵黄蛋白原试剂盒         96孔/盒                                      

QYD621   麻痹性贝类毒素（PSP）试剂盒   96孔/盒                                      

QYD622   腹泻性贝类毒素（DSP）试剂盒  96孔/盒                                      

QYD623   健忘性贝类毒素（ASP）试剂盒  96孔/盒                                      

QYD624   组胺试剂盒     96孔/盒                                       【人游离三碘甲状腺原氨酸(Free-T3)ELISA试剂盒厂家】

QYD625   葡萄糖试剂盒         96孔/盒                                      

QYD626   果糖葡萄糖试剂盒         96孔/盒                                      

QYD627   氨氮试剂盒     96孔/盒                                      

QYD628   硝酸盐亚硝酸盐试剂盒         96孔/盒                                      

QYD629   维生素C试剂盒     96孔/盒                                      

QYD630   尿素试剂盒     96孔/盒                                      

QYD631   果糖试剂盒     96孔/盒                                      

QYD632   皮革水解蛋白试剂盒     96孔/盒                                      

QYD633   物种鉴定（孔测)试剂盒        96孔/盒                                      

QYD634   物种鉴定(管测)试剂盒  96孔/盒                                      

QYD635   多菌灵试剂盒         96孔/盒                                      

QYD636   氟乐灵试剂盒         96孔/盒                                      

QYD637   病毒DNA/RNA磁珠提取试剂盒   100次/盒                                             

QYD638   细菌基因组DNA磁珠提取试剂盒        100次/盒                                             

QYD639   禽流感病毒通用荧光定量RT-PCR检测试剂盒   48次/盒                                      

QYD640   禽流感H5亚型荧光定量RT-PCR检测试剂盒    48次/盒                                       【人游离三碘甲状腺原氨酸(Free-T3)ELISA试剂盒厂家】

QYD641   禽流感H7亚型荧光定量       48次/盒                                      

QYD642   禽流感H9亚型荧光定量RT-PCR检测试剂盒     48次/盒                                      

74104       RNeasy Mini Kit (50)         50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml ａnd 2 ml), RNase-free Reagents ａnd Buffers         CNY                             

72251       TurboCapture 96 mRNA Kit (5)         For rapid ａnd easy mRNA purification in high-throughput applications. The kit provides a fast ａnd simple procedure for purifying mRNA. Up to 96 samples can be processed simultaneously - ideal for high-throughput applications such as compound screening ａnd target validation (e.g. by siRNA screening).   CNY                             

19407       VacConnectors (500)        500 disposable connectors for use with QIAamp spin columns on luer connectors. Should be ordered in conjuntion with 19408 - VacValves (24).         CNY                             

12281       QIAfilter Plasmid Mega Kit (5)          5 QIAGEN-tip 2500, Reagents, Buffers, 5 QIAfilter Mega-Giga Cartridges     CNY                             

12191       QIAGEN Plasmid Giga Kit (5)   5 QIAGEN-tip 10000, Reagents, Buffers CNY                             

4502103  artus VZV TM PCR Kit (24) RUO                CNY                             

133271     NeXtalStock PIPES pH 7.0 (200)                 CNY                             

133270     NeXtalStock Phosphate-citr. pH 5.2 (200)                 CNY                              【人游离三碘甲状腺原氨酸(Free-T3)ELISA试剂盒厂家】

990512     Filter-Tips, 50 µl (960)               CNY                             

337620     qBiomarker SYBR Green Fluor Mastermix(2)           CNY                             

337011     qBiomarker Somatic Mutations PCR Assays              CNY                             

4500263  artus HSV-1/2 RG PCR Kit (24) CE               CNY                             

939016     Buffer ATL ( 4 x 50 ml),GPR               CNY                             

4500163  artus HSV-1/2 TM PCR Kit (24) CE              CNY                             

1073925  Ni-NTA Superflow (8 x 500 ml),cus,G                 CNY                             

9010931  BGR Greifer P7 (alt)                   CNY                             

9010858  BGR DT-Abstreifer 8-fach P7xxx D            CNY                             

931036     QIAsymphony Virus/Bact Mini Kit (192)           CNY                             

133248     NeXtalStock Sodium succinate pH6.5 (200)              CNY                             

330603     RT² SYBR Green FAST Mastermix (24)               CNY                             

929608     QX Pro Separation Buffer (40 ml)              CNY                             

5000-1415        Knob RB671CSKWL Matte, VTX2              CNY                             

5000-1408        Cable Shielded 1/17HP, VTX2             CNY                             

L2170       hc2 CT/GC HC2 DNA Test PI (CD-ROM)             CNY                              【人游离三碘甲状腺原氨酸(Free-T3)ELISA试剂盒厂家】

L1971       Rotary Shaker User＇s Manual           CNY                             

9240705  O-ring Kit, Capper, Spindle, AP                  CNY                             

9240693  Actril Sterilant & Test Strips, AP                CNY                             

9243653  Comprehen Valid., Other Extraction Sys """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   The Customer is expected to have all materials on-hand 1 week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """     CNY                             

386516     Investigator STR GO! Lysis Buffer (200)            CNY                             

208256     QuantiNova Probe PCR Kit (2500)             CNY                             

208254     QuantiNova Probe PCR Kit (500)               CNY