【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【人preptinELISA试剂盒现货】样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【人preptinELISA试剂盒现货】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

【人preptinELISA试剂盒现货】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【人preptinELISA试剂盒现货】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

QR20-74 Taq DNA Polymerase     1000U                                

QR20-75 2 x Taq MasterMix 1ml                             

QR20-76 2 x Taq MasterMix 3ml                             

QR20-77 Pfu DNA Polymerase     250U                                  

QR20-78 Pfu DNA Polymerase     500U                                  

QR20-79 2 x Pfu MasterMix 1ml                             

QR20-80 2 x Pfu MasterMix 3ml                              【人preptinELISA试剂盒现货】

QR20-81 HotStar Taq DNA Polymerase       125U                                  

QR20-82 HotStar Taq DNA Polymerase       500U                                  

QR20-83 2 x HotStar Taq MasterMix   1ml                             

QR20-84 2 x HotStar Taq MasterMix   3ml                             

QR20-85 Oligo(dT)15    500μl(10μM)                             

QR20-86 M-MLV反转录酶  10000U                              

QR20-87 M-MLV反转录酶  50000U                              

QR20-88 M-MLV cDNA链合成试剂盒      20次                                 

QR20-89 M-MLV cDNA链合成试剂盒      50次                                 

QR20-90 Thermo M-MLV Reverse Transcriptase       10000U                              

QR20-91 Thermo M-MLV Reverse Transcriptase       50000U                              

QR20-92 Thermo M-MLV cDNA链合成试剂盒 20次                                 

QR20-93 Thermo M-MLV cDNA链合成试剂盒 50次                                 

QR20-94 实时荧光定量 PCR MasterMix    1ml                             

QR20-95 实时荧光定量 PCR MasterMix    3ml                             

QR20-96 RealSuper二步法荧光定量PCR试剂盒     20次                                  【人preptinELISA试剂盒现货】

QR20-97 RealSuper二步法荧光定量PCR试剂盒     100次                                

QR20-98 pUX-T TA 克隆试剂盒 20次                                 

QR20-99 pUX-T simple TA 克隆试剂盒     20次                                 

QR20-100      加A试剂盒   20次                                 

QR20-101      T4连接酶      200U                                  

QR20-102      0感受态细胞       10 x 100μl                               

QR20-103      0感受态细胞       20 x 100μl                               

QR20-104      DH5α感受态细胞 10 x 100μl                               

QR20-105      DH5α感受态细胞 20 x 100μl                               

QR20-106      BL21(DE3)感受态细胞 10 x 100μl                               

9240948  Cable, x, portal, Qsym          CNY                           

9240825  Field Upgrade, BR EZ1 DSP         CNY                           

9240817  #Premium Cover Agreement EZ1 DSP, 1YR "Repair service delivery: onsite

Service response time (repair): 24 working hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"      CNY                           

9240776  Dispense Head Update, AP96        CNY                           

9022763  EZ1 Adv. XL DNA Invest. Flip-Cap Card            CNY                           

6000-5012      Lumicheck Battery Replacement Package            CNY                           

6000-5005      Micro Well Strips          CNY                           

9241774  QIAgility, Installation     The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It covers the handling of the QIAgility systems hardware ａnd trains the user on the operation of the software. After the training the user will be able to run ａnd install application programs ａnd conduct a basic troubleshooting. All travel ａnd labor costs are included. The service is documented in accordance with GMP/GLP requirements.   CNY                           

9241655  Input Rack Pins Kit, AP        CNY                           

9241360  Plastic supporters, door hinge, EZ1a            CNY                           

9243547  Pyro Q24, PM Service   "

Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage" CNY                           

9243112  Hard Drive, Programmed, Cheetah, AP98            CNY                           

9242770  Belt, x-drive, portal, AS, Qsym            CNY                           

9242698  RGQ, Full Agreement     "Repair service delivery: send to Regional Repair Center

Service response time (repair): 7-10 days turnaround time

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: yes, included (provision time 2 days)

Transportation cost coverage: yes, included"       CNY                           

5000-1406      Control PCB 120V, VTX2             CNY                           

5000-1320      Label Front Overlay, SKR1           CNY                            【人preptinELISA试剂盒现货】

5000-1312      Platform, SKR1             CNY                           

5000-1274      Plate Mask, DML2        CNY                           

5000-1253      Iris Control PCB, DML2              CNY                           

5050-1071 V.1       CD RomManual-DML2000 Instrument I, 240V          CNY                           

5050-00031    CONVERTER USB        CNY                           

5000-1509      Control Heater, HTR1           CNY                           

6000-3121      Plate Washer 1 - Replacement Tubing Kit            CNY                           

6000-3115      Plate Washer I - Manifold Accessory Kit             CNY                           

5000-1502      Fuse 250V 1A, HTR1           CNY                           

5000-1419      Breaker Circuit 1Pole, VTX2        CNY                           

5198-1220      hc2 HPV DNA Test(High Risk ａnd Low Risk)            CNY                           

EA10224 EASYartus VZV RG_II         CNY                           

EA10023 EASYartus HSV-1/2 RG_I            CNY                           

PK-01     Mutation Test Kit (25)           CNY                           

9240744  Belt, Reagent Pump , Long, AP96         CNY