检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供*优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【人过氧化物酶体增殖因子活化受体γ(PPAR-γ)ELISA试剂盒厂家】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人过氧化物酶体增殖因子活化受体γ(PPAR-γ)ELISA试剂盒厂家】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在、第二孔中分别加标准品100μl,然后在、第二孔中加标准品稀释液50μl,混匀；然后从孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【人过氧化物酶体增殖因子活化受体γ(PPAR-γ)ELISA试剂盒厂家】

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品*终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果. 【人过氧化物酶体增殖因子活化受体γ(PPAR-γ)ELISA试剂盒厂家】

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请*后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

COX  细胞色素C氧化酶含量试剂盒     Cytochrome C Oxidase Assay   100Tests in cuvette                                    

CS     柠檬酸合成酶的检测试剂盒         Citrate Synthase Assay     100Tests in cuvette                                    

NO   一氧化氮色标法检测试剂盒        Colorimetric Nitric Oxide Assay        250 tests/96 well plate                                

TACA         总抗氧化能力检测试剂盒     Total Antioxidant Capacity Assay     100 tests                                     

SOD 超氧化物歧化酶分析试剂盒       Superoxide Disutase Assay       100 tests                             

CAT  过氧化氢酶活性分析试剂盒       Catalase Activity Assay    100 tests                             

GPx  谷胱甘肽过氧化物酶分析试剂盒         Glutathione Peroxidase Assay 100 tests                             

NAD "NAD/NADH 含量检测试剂盒

"       "NAD/NADH Assay

"       100 Tests in 96-well plate                                    

XO   "黄嘌呤氧化酶检测试剂盒

"       Xanthine Oxidase Assay         100 Tests in 96-well plat                             

TGA 总谷胱甘肽含量检测试剂盒         Total Glutathione Assay           100 tests                             

Ared          茜素红S染色试剂盒     Alizarin Red S Staining Kit        100 ml                                

HGL PCR  人胚层检测试剂盒         Human Germ Layer Detection Kit   50 reactions                               

Ored         油红O染色试剂盒         Oil Red O Staining Kit       100 ml                                

HSC PCR   人干细胞多能性检测试剂盒         Human Stem Cell Pluripotency Detection Kit  50 reactions                                【人过氧化物酶体增殖因子活化受体γ(PPAR-γ)ELISA试剂盒厂家】

ALP  碱性磷酸酶活性测定试剂盒         Alkaline Phosphatase Activity Assay         500 tests                                     

ALPr 碱性磷酸酶染色检测（红色）     Alkaline Phosphatase Staining Assay (Red)      500 tests                                     

PCR  人间质干细胞成脂肪细胞检测试剂盒         Human Mesenchymal Stem Cell Adipogenesis Detection Kit  50 reactions                                

HMSC-C PCR     人间充质干细胞软骨形成检测试剂盒         Human Mesenchymal Stem Cell Chondrogenesis Detection Kit      50 reactions                                    

SafraninO          番红O染色试剂盒         Safranin O Staining Kit     100 reactions                                      

ABlue        阿利新蓝染色试剂盒     Alcian Blue Staining Kit    100 reactions                                      

         └ 肺微血管内皮细胞总RNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 肺大动脉平滑肌细胞总RNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 肺大动脉内皮细胞总RNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 肺大静脉内皮细胞总RNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 肺大静脉平滑肌细胞总RNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 肺动脉成纤维细胞总RNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 肺泡上皮细胞总RNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 气管上皮细胞总RNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 支气管上皮细胞总RNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 小气道上皮细胞总RNA  1μg/1μl（10μg/20ug/50ug）                                          【人过氧化物酶体增殖因子活化受体γ(PPAR-γ)ELISA试剂盒厂家】

         └ 肺成纤维细胞总RNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 支气管平滑肌细胞总RNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 小气道平滑肌细胞总RNA       1μg/1μl（10μg/20ug/50ug）                                         

9237135  #Twister I, Full Agreement, no PM The Full Agreement BR Twister I provides full service coverage for the BioRobot Twister I. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Full Agreement, there is a priority response time of 48 hours (2 working days).       CNY                             

9237109  Protocol Dev./Opt., on-site (5-days)         The QIAGEN BioRobot Systems Protocol Development/Optimization On-Site Visit includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Instrumentation Service Specialist to write and/or opimize protocols for the BioRobot System. Parts, consumable chemistries, ａnd accessories not included. Custom protocols are the sole responsibility of the customer ａnd neither warrantied nor guaranteed.          CNY                             

9237102  Upgrade, BR plus 2 ext. devices       Upgrade BioRobot with two approved integration devices    CNY                             

9232974  Card, x-main, peristaltic XPUPG                CNY                             

9232972  YZ 96 CPU Board/Driver Unit            CNY                             

9232947  Springcontact, RoboVac, BR8000             CNY                             

9236408  Storage Platform TW I, deep well             CNY                             

9232918  Board, PCON, auto vac, BR8000               CNY                             

9236334  Green LSR                  CNY                              【人过氧化物酶体增殖因子活化受体γ(PPAR-γ)ELISA试剂盒厂家】

9236324  Fan, LiquiChip            CNY                             

9236151  Locator Plate, 96 well,pos 2,BR RP          CNY                             

9230820  Rack for reagents, Universal (zig-zag)              CNY                             

9230718  Predilution-Rack 320x2ml Rose-80           CNY                             

9230365  Cable,Interface,flat,(Syr.)-Incub.-Read.            CNY                             

9021284  Safety lock, waste drawer, v1, Qsym                 CNY                             

9238611  Rubber seal, centrifuge motor                  CNY                             

9238477  Tubing, OLF pump head, RapidPlate                  CNY                             

9238245  Tray for Reaction               CNY                             

9238191  Upgrade App., DirectPrep 96           CNY                             

9237721  EZ1, Full Agreement         The Full Agreement EZ1 provides full service coverage for the BioRobot EZ1. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).   CNY                            

9240676  Bimba Cylinder, Latch Lock, AP98             CNY                             

9240668  Nut, Flangeless,1/4-28, Blue, AP                CNY                             

9240632  Power Supply, 48VDC 4.0 AMP, AP96                CNY                             

9240605  Pressure Gauge, Digital, AP196/98           CNY                             

9240602  Sensor, Sick, Waste Btl. Present, AP                  CNY                              【人过氧化物酶体增殖因子活化受体γ(PPAR-γ)ELISA试剂盒厂家】

9240561  Control Pad, Eppendorf, AP196/98            CNY                             

9240558  Temp sensor, Bowl, Eppendorf, AP196/98                  CNY                             

9240540  Right Angle Con., SiNug, Motor, AP96/196                CNY                             

9240535  Motor Assy, SiNug, 23, Pump, AP96/196           CNY                             

9240517  Cable, Dual, USB transition, AP196/98              CNY                             

9240195  Holder, click＇n go cassettes, HSPS             CNY