1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

【人可溶性核因子κB受体活化因子配基(sRANKL)ELISA试剂盒现货】齐一生物【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

ELISA应用：

ELISA检测试剂盒、ELISAKit、酶联免疫试剂盒、酶联免疫吸附测定试剂盒、酶联免疫分析试剂盒、酶免试剂盒等,比较常见的叫法是ELISA检测试剂盒、酶联免疫吸附测定试剂盒等ELISA试剂盒自从60-70年代问世以来,得到全世界科研工作者的认可及推崇,在欧美及中国获得很大的推广,尤其是国内生化领域的长足发展.Elisa生物试验是一种敏感性高,特异性强,重复性好的实验诊断方法.由于其试剂稳定、易保存,操作简便,结果判断较客观等因素,已广泛应用在免疫学检验的各领域中.ELISA检测试剂盒适用于体外定性检测人血清或血浆中的抗人类戊型肝炎（HEV）病毒IgM抗体ELISA检测

【人可溶性核因子κB受体活化因子配基(sRANKL)ELISA试剂盒现货】齐一生物科技欢迎您订购咨询，齐一生物销售：021－6034 8496；181214 53965；173021 04490

齐一生物供应的ELISA试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【人可溶性核因子κB受体活化因子配基(sRANKL)ELISA试剂盒现货】齐一生物供应的ELISA试剂盒ELISA实验原理

试剂盒是固相夹心法酶联免疫吸附实验（ELISA）.已知浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测.先将生物素标记的抗体同时温育.洗涤后,加入亲和素标记过的HRP.再经过温育和洗涤,去除未结合的酶结合物,然后加入底物A、B,和酶结合物同时作用.产生颜色.颜色的深浅和样品中的浓度呈比例关系.

免疫测定技术的基础在于抗原抗体之间的特异结合反应,所以任何优质的诊断试剂离不开优质的原料,如抗原抗体,酶等.以前用于免疫测定的抗原通常为各种纯化抗原,而抗体则为纯化抗原免疫动物后获得的多克隆抗体和使用杂交瘤技术得到的单克隆抗体,而抗原或抗体的标记物如酶标结合物则通过各种化学合成方法制备.近年来,随着分子生物学的发展,使用基因工程方法制备各种特殊的抗原或抗体及其酶结合物等免疫测定试剂几成为现实的新一代试剂,各种新型使用的测定方法也不断出现.

HBsAg试剂特点（检测模式：临床抗体夹心法）：包被抗体为山羊多抗.多抗具有高亲和性和对抗原各种表位的反应性.酶表抗体为与HBsAg有不同结合位点的鼠复合单位,可测得HBsAg变异样品,提高检测的特异性（99.98%）提高检测的灵敏度,应用ParlEhrich学说（PEI）HBsAg标准品,对ad标准品的灵敏度为0.05ng/ml对ay标准品灵敏度为0.025ng/ml

ELISA检测试剂盒应用定性夹心免疫检测技术,用合成的HEV多肽抗原包被微孔板板条,这些多肽是中国型HEV毒株核心氨基酸序列中抗原性很强的肽段,分别来自于该毒株的开放阅读框2和开放阅读框3.将样品或标准品加入孔中并孵育,如果其中存在HEVIgM抗体,这些抗体就会与HEV的多肽抗原结合,并固定在上面,洗板除去其它非特异性抗体和样品中的其它成份.然后加入羊抗人IgM-HRP（辣根过氧化物酶）酶结合物,经第二次孵育后,酶结合物就会与次孵育结合上的HEVIgM抗体相结合,洗板除去未结合的酶结合物,加入TMB底物溶液,在第三次孵育时会发生酶-底物反应,只有那些含有HEVIgM抗体和酶结合物所形成的复合物的孔才会发生颜色变化,加入硫酸溶液终止酶和底物间的反应,并在波长:450nm处测量O.D.值,按照本HEVIgM抗体elisa试剂盒的测试标准,O.D.值大于或等于Cut-Off值的样品被认为是初试阳性. 【人可溶性核因子κB受体活化因子配基(sRANKL)ELISA试剂盒现货】

以上资料仅供参考了解，不供于论文写作

齐一生物专业为您提供高品质，高灵敏度.高质量的酶联免疫试剂盒，齐一生物长期经营ELISA试剂盒（免费代测）及抗体、分子学生物试剂盒、金标法检测试剂盒、比色法检测试剂盒、细胞菌株、培养基、细胞因子、生化试剂、耗材等生物试剂产品。齐一生物销售：021－6034 8496；181214 53965；173021 04490

QT-00384 大丽花轮枝孢ACCC30308      冻干粉                                         

QT-00385 白浅灰链霉菌ACCC40001      冻干粉                                         

QT-00386 抗生链霉菌ACCC40070 冻干粉                                         

QT-00387 天蓝色链霉菌ACCC40009      冻干粉                                         

QT-00388 淡天蓝色链霉菌ACCC40010 冻干粉                                         

QT-00389 黄直丝链霉菌ACCC40046      冻干粉                                         

QT-00390 弗氏链霉菌ACCC40012 冻干粉                                         

QT-00391 刺孢吸水链霉菌ACCC40018 冻干粉                                         

QT-00392 紫色直丝链霉菌ACCC40032 冻干粉                                         

QT-00393 刺孢吸水链霉菌昆明变种ACCC40020 冻干粉                                         

QT-00394 吸水链霉菌紫色变种ACCC40034 冻干粉                                         

QT-00395 淡紫灰链霉菌ACCC40024      冻干粉                                         

QT-00396 孔雀石绿链霉菌ACCC40026 冻干粉                                          【人可溶性核因子κB受体活化因子配基(sRANKL)ELISA试剂盒现货】

QT-00397 玫瑰褐链霉菌ACCC40123      冻干粉                                         

QT-00398 珊瑚色诺卡氏菌ACCC40100 冻干粉                                         

QT-00399 鸡腿蘑（毛头鬼伞）ACCC50400  冻干粉                                         

QT-00400 鸡油菌ACCC50878 冻干粉                                         

QT-00401 宽圆羊肚菌ACCC50759 冻干粉                                         

QT-00402 长根菇（长根奥德磨）ACCC50053       冻干粉                                         

QT-00403 异型酒香酵母ACCC20234      冻干粉                                         

QT-00404 嗜酸乳杆菌ACCC11073 冻干粉                                         

QT-00405 巨大芽孢杆菌ACCC11011      冻干粉                                         

QT-00406 褐球固氮菌ACCC10096 冻干粉                                         

QT-00407 枯草芽孢杆菌ACCC10157      冻干粉                                         

QT-00408 红平红球菌ACCC10543 冻干粉                                         

QT-00409 谷氨酸棒杆菌ACCC16522      冻干粉                                         

QT-00410 蜡蚧轮枝孢菌ACCC32146      冻干粉                                         

QT-00411 泾阳链霉菌ACCC40126 冻干粉                                         

QT-00412 诺尔斯氏链霉菌ACCC40029 冻干粉                                          【人可溶性核因子κB受体活化因子配基(sRANKL)ELISA试剂盒现货】

QT-00413 吸水链霉菌应城变种ACCC40053 冻干粉                                         

QT-00414 刺孢吸水链霉菌北京变种ACCC40074 冻干粉                                         

QT-00415 浅灰链霉菌杭州变种ACCC40075 冻干粉                                         

QT-00416 铜绿假单胞杆菌（绿脓杆菌）     ATCC 27853      支     上海         400           

9013308  Base, vacuum manifold, v1.04                   CNY                             

9013298  Top plate, vacuum manifold96 plate , 4           CNY                             

9013236  Tubing set, vacuum, 200, 0               CNY                             

9013224  Gripper set, V-handler, BR3000                 CNY                             

9013197  Tubing, L1 outlet, auto vac,BR8000 v1.00                CNY                             

9011702  Cable, PCON98 to AC-box (level sensor),          CNY                             

9011681  Dispenser drive, BR8000          CNY                             

132635     NeXtal Stock Zinc chloride (50)        0,1M Zinc chloride - 50mL (4 x 12,5 ml tube)  CNY                             

132578     NeXtal Stock PEG 2,000 DME (50)   50% w/v Polyethylene glycol 2,000 dimethyl ether - 50mL (4 x 12,5 ml tube)         CNY                             

800341     Cosmid End-Sequencing, w/ prep              CNY                              【人可溶性核因子κB受体活化因子配基(sRANKL)ELISA试剂盒现货】

800308     siRNA Hairpin Run w/ prep                CNY                             

800121     Verification Sequencing, w/ prep     "Double-stranded sequencing of a given sequence

QIAGEN® DNA template preparation

Design ａnd synthesis of walking primers

Data assembly ａnd sequence alignment

Accuracy >99.99%" CNY                             

132528     NeXtal Stock CAPSO untit. (50)         0.5M CAPSO - Untitrated - 50mL (4 x 12,5 ml tube)         CNY                             

132510     NeXtal Stock ADA untit. (50)   0.5M ADA - Untitrated - 50mL (4 x 12,5 ml tube)   CNY                             

764114     PAXgene Bone Marrow RNA Tubes (50)           CNY                             

679913     ipsogen RT Kit (33)            CNY                             

203643     HotStarTaq Plus Master Mix Kit (250)     "For 250 x 20 µl reactions: 3 x 0.85 ml HotStarTaq Plus Master Mix, containing 250 units of HotStarTaq Plus DNA Polymerase total, 1 x 0.55 ml CoralLoad Concentrate, 2 x 1.9 ml RNase-free Water

HotStarTaq Plus Master Mix Kits offer all the benefits of great specificity, sensitivity, ａnd performance as HotStarTaq Plus DNA Polymerase NOW in a ｍaster mix format, which offers even faster results ａnd added convenience."  CNY                             

201225     Taq PCR Core Kit (1000)   "4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2, dNTP Mix†

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."         CNY                             

201203     Taq DNA Polymerase (250)      "250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."         CNY                             

180922     GeneRead Adapter L Set 1plex (12)                   CNY                             

180912     GeneRead Adapter I Set 1plex (12)          CNY                             

159214     QIAcard FTA Indicator Four Spots (25)              CNY                             

159201     QIAcard FTA One Spot (100)              CNY                             

9235523  Needle cleaning assembly P/N 4567-01            CNY                             

9235300  Plunger tip, syringe 2,5ml                  CNY                             

9235117  Fuse F4A, v1.00                  CNY                              【人可溶性核因子κB受体活化因子配基(sRANKL)ELISA试剂盒现货】

9022818  EZ1 Advanced XL Flip Cap Tube Rack                 CNY                             

9234889  Vacuum manifold, 96 seal                  CNY                             

9234862  System liquid container (2l Nalgene)                 CNY                             

9239555  PCBA, Laser Driver, Unlimited          CNY                             

9238664  Hinge                 CNY                             

9020593  Lid, DML3000           CNY                             

9022383  Original Transport Box, QXA             CNY                             

QT-00316 米曲霉CICC2014     冻干粉                                         

QT-00317 弗氏柠檬酸杆菌CICC20211  冻干粉                                         

QT-00318 谢氏丙酸杆菌CICC10019       冻干粉                                         

QT-00319 许旺酵母变种CCRC20332      冻干粉                                         

QT-00320 大肠杆菌CCRC11447      冻干粉                                         

QT-00321 DSMZ18235     冻干粉                                         

QT-00322 巨大芽孢杆菌ACCC11107      冻干粉                                         

QT-00323 球型芽孢杆菌ACCC11096      冻干粉                                         

QT-00324 枯草芽孢杆菌ACCC10627      冻干粉                                         

QT-00325 黄色短杆菌ACCC11063 冻干粉                                         

QT-00326 产黄纤维单胞菌ACCC11055 冻干粉                                         

QT-00327 保加利亚乳杆菌ACCC10638 冻干粉                                         

QT-00328 干酷乳杆菌ACCC10639 冻干粉                                         

QT-00329 植物乳杆菌ACCC11095 冻干粉                                          【人骨钙素/骨谷氨酸蛋白(OT/BGP)ELISA试剂盒现货】

QT-00330 变形杆菌ACCC11002      冻干粉                                         

QT-00331 恶臭假单胞菌ACCC10085      冻干粉                                         

QT-00332 藤黄八叠球菌ACCC11001      冻干粉                                         

QT-00333 乳酸链球菌ACCC10653 冻干粉                                         

QT-00334 乳酸链球菌ACCC11092 冻干粉                                         

QT-00335 乳酸链球菌ACCC11093 冻干粉                                         

QT-00336 乳酸链球菌ACCC11094 冻干粉                                         

QT-00337 蜡状芽孢杆菌ACCC11077      冻干粉                                         

QT-00338 荧光威克酵母ACCC20058      冻干粉                                         

QT-00339 间型酒香酵母ACCC20233      冻干粉                                         

QT-00340 葡萄芽假丝酵母ACCC20201 冻干粉                                         

QT-00341 近平滑假丝酵母ACCC20221 冻干粉                                         

QT-00342 热带假丝酵母ACCC20005      冻干粉                                         

QT-00343 产朊假丝酵母ACCC20060      冻干粉                                         

QT-00344 汉逊德巴利酵母ACCC20010 冻干粉                                         

QT-00345 健强地霉ACCC20017      冻干粉                                          【人骨钙素/骨谷氨酸蛋白(OT/BGP)ELISA试剂盒现货】

QT-00346 阿拉伯糖醇汉逊酵母ACCC20205 冻干粉                                         

QT-00347 杰丁汉逊酵母ACCC20019      冻干粉                                         

9011608  BGR Kamm für Stirrer PK80 neu                CNY                             

9013178  Gripper set, BR8000 v 1.00               CNY                             

9013170  Tubing, syringe inlet 2, BR8000                 CNY                             

9011417  Klemmplättchen Varispan P7 (Alodine)             CNY                             

5050-1020EN   #Keyboard English Spare          CNY                             

9240731  Tubing, 3/8"OD , Blue, AP                   CNY                             

676091     ipsogen CBFB-MYH11 A Standards           CNY                             

9011341  Microplate Washer Module Plato 3                   CNY                              【人骨钙素/骨谷氨酸蛋白(OT/BGP)ELISA试剂盒现货】

9011203  Vacuum control unit, BR8000           CNY                             

673522     ipsogen JAK2 MutaQuant Kit (12) CE                 CNY                             

337839     qBiomarker SYBR Fluor Mastermix 25 ml                  CNY                             

337632     qBiomarker SYBR Green ROX Mastermix (12)                   CNY                             

9018907  36-Well Rotor            CNY                             

9018783  CAT, Qsym                  CNY                             

9018694  EZ1 Adv. XL DNA Bacteria Card                 CNY                             

9018565  Door lock, mechanical, EZ1a            CNY                             

9013132  Elevating Platform            CNY                             

4509063  artus WNV LC RT-PCR Kit (24) CE              CNY                             

9001905  #QIAgility (w/o PC) System       QIAgility (w/o PC) System combines the QIAgility instrument ａnd installation. The Installation plus Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.    CNY                             

800383     Open Reading Frame Search            CNY                             

9019052  Notebook 2 XP          CNY                             

9020200  ZLV PCB, USB, 18Q            CNY                             

9020580  Cable, DB9 M/F (UL) 6ft, DML 3000                  CNY                             

9020588  Pulley, belt, DML 3000              CNY                              【人骨钙素/骨谷氨酸蛋白(OT/BGP)ELISA试剂盒现货】

9021016  Robot Stage, X Axis, VR, DCU           CNY                             

9013376  Maintenance Kit BR 8000 Research                  CNY                             

9013279  BGR Upgrade-Kit Scanner PK80                CNY                             

9013272  Peristaltic Pump, RCS1              CNY                             

9013190  Motor, rotor, buffer track, BR8000          CNY                             

132772     NeXtal Stock Jeffamine M-600 (200)        100% v/v O-(2-Aminopropyl)-O＇-(2-methoxyethyl)polypropylene 500 - 200mL (20 x 10 ml tube)       CNY                             

132620     NeXtal Stock Tricine (50) 1M Tricine - 50mL (4 x 12,5 ml tube)        CNY