检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供*优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【人吡哆醛/吡哆醇维生素B6磷酸酶(PDXP)ELISA试剂盒现货】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

【人吡哆醛/吡哆醇维生素B6磷酸酶(PDXP)ELISA试剂盒现货】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在、第二孔中分别加标准品100μl,然后在、第二孔中加标准品稀释液50μl,混匀；然后从孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品*终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

【人吡哆醛/吡哆醇维生素B6磷酸酶(PDXP)ELISA试剂盒现货】注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果.

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请*后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

【人吡哆醛/吡哆醇维生素B6磷酸酶(PDXP)ELISA试剂盒现货】计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

QY-18542 Anti-Tenascin/TN细胞粘合素抗体                                                       

QY-18552 Anti-TFF3 (trefoil factor3)三叶肽因子3抗体                                                       

QY-18562 Anti-TFPI-2 (tissue factor pathway in hibitor-2)组织因子途径抑制剂-2抗体                                                     

QY-18572 Anti-TGF-α (Ttansforming Growth Factor –α)转移生长因子–α抗体                                               

QY-18582 Anti-TGF-β1（Ttansforming Growth Factor –β1）转移生长因子–β1抗体                                                     

QY-18592 Anti-TGF-β2 （Ttansforming Growth Factor –β2）转移生长因子–β2抗体                                                   

QY-18602 Anti-TGF-β3（Ttansforming Growth Factor –β3）转移生长因子–β3抗体                                                     

QY-18612 Anti-TGF-βR 2（Transforming growth factor β receptor 2 ）转移生长因子–β受体2抗体                                                   

QY-18622 Anti-TGF-βR1/ALK (TGF-beta receptor type I)转移生长因子–β受体1抗体                                                  【人吡哆醛/吡哆醇维生素B6磷酸酶(PDXP)ELISA试剂盒现货】

QY-18632 Anti-Thioster-containing protein 1（Anti-Anopheles stephensi protein 1）硫酯包含蛋白-1抗体                                                    

QY-18642 anti-Thioredoxin 1(ERdj5)硫氧还蛋白抗体                                                  

QY-18652 Anti-Thy-1/CD90CD90抗体                                                   

QY-18662 Anti-Thymosin α-1胸腺肽 α-1抗体                                               

QY-18672 Anti-Thyroxine(T4)/Tg4-Ab (Thyroglobulin 4 Antibody)甲状腺素T4抗体                                                    

QY-18682 anti-Tie2（Porcine tyrosine kinase with immunoglobulin-like ａnd EGF-like domains 2）上皮生长因子样域酪氨酸激酶2                                                    

QY-18692 Anti-TIGAR humen (TP53- induced glycolysis ａnd apoptosis-regulator )兔抗人TIGAR蛋白抗体                                               

QY-18702 Anti-TIMP-1(Tissue Inhibitor of Metalloproteinase-1)金属蛋白酶组织抑制因子-1抗体                                                  

QY-18712 Anti-TIMP-2(Tissue Inhibitor of Metalloproteinase-2)金属蛋白酶组织抑制因子-2抗体                                                  

QY-18722 Anti-TIMP-3(Tissue Inhibitor of Metalloproteinase-3)金属蛋白酶组织抑制因子-3抗体                                                  

QY-18732 Anti-TIMP-4(Tissue Inhibitor of Metalloproteinase-4)金属蛋白酶组织抑制因子-4抗体                                                  

QY-18742 Anti-TLR2 (Toll-like receptor 2)Toll样受体2抗体                                               

QY-18752 Anti-TLR4 (Toll-like receptor 4)Toll样受体4抗体                                               

QY-18762 Anti-TLR5/CD285 (Toll-like receptor 5)Toll样受体5抗体                                                     

QY-18772 Anti-TM（Thrombomodulin)血栓调节蛋白抗体                                                

QY-18782 Anti-TmR2（GDNFRβ；TRN-R；GFR-alpha-2；NTNR-2）胶质细胞系源性神经营养因子受体β抗体                                                【人吡哆醛/吡哆醇维生素B6磷酸酶(PDXP)ELISA试剂盒现货】

QY-18792 anti-Tn-C(tenascin-C)腱糖蛋白-C抗体                                                 

QY-18802 Anti-TNF-α (Mouse Anti-Human Tumor Necrosis Factor-α Monoclonal Antibody)鼠抗人肿瘤坏死因子-α单克隆抗体                                                       

QY-18812 Anti-TNF-α(Tumor Necrosis Factor-α)肿瘤坏死因子-α抗体                                                   

QY-18822 Anti-TNF-β(Tumor Necrosis Factor-β)肿瘤坏死因子-β抗体                                                   

QY-18832 Anti-TPⅠ （topoisomeraseⅠ）拓普西异构酶Ⅰ抗体                                                     

QY-18842 Anti-DNA TPⅡα (DNA topoisomeraseⅡα)DNA拓普西异构酶Ⅱ抗体                                                  

QY-18852 Anti-TPH (Trptophan Hydroxylase)色氨酸羟化酶抗体                                                

QY-18862 Anti-TPO (Thyroid Peroxidase)抗甲状腺过氧化物酶抗体                                                   

972442     PyroMark Q96 SNP HFE (96)    For detection of variants in HFE gene      CNY                             

133221     NeXtalStock Sodium ac. H2O pH 5.6 (200)                CNY                             

133220     NeXtalStock Sodium ac. H2O pH 5.0(200)                 CNY                             

301704     HiPerFect Transfection Reagent (0,5 ml)                   CNY                             

301427     Effectene Transfection Reagent (4x1ml) 4 x 1 ml Effectene Reagent, Enhancer, Buffer; for 160 transfections in 60 mm dishes or 640 transfections in 12-well plates      CNY                             

51326       QIAamp DNA Mini QIAcube Kit (240)                CNY                             

9012672  BGR Peripumpe PP-4 Frontbl.gerade (7135)            CNY                             

9002011  Rotor-Gene Q MDx 2Plex HRM no PC               CNY                             

292063     mericon Sheep Kit (24)              CNY                             

9001912  PyroMark Q24 MDx, PrioPLUS         "PyroMark Q24 MDx Instrument

Installation of PyroMark Q24  & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10

Loaner available in 2 days*

Annual Inspections (1 onsite PM per yearr)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)

(In the event of shipping logistic limitation, QIAGEN reserves the option to repair the instrument on-site. Response time 48 Hrs)"         CNY                             

9001910  QIAgility HEPA/UV (w/o PC) PrioPLUS      "QIAgility HEPA/UV (w/o PC)  Instrument

Installation of QIAgility instrument & Introductory training

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite pipetting head service ａnd inspection per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

133116     NeXtalStock Sodium thiocyanate (200)             CNY                             

967142     RNeasy 96 BioRobot 9604 Kit (12)  For 12 x 96 total ａnd cytoplasmic RNA preps on the BioRobot 9604: 12 RNeasy 96 Plates, Elution Microtubes CL (1.2 ml), Caps, Square-Well Blocks, RNase-free Reagents ａnd Buffers         CNY                             

962141     QIAprep 96 Turbo BioRobot Kit (4)  For 4 x 96 high-purity plasmid minipreps, 4 each: TurboFilter 96 ａnd QIAprep 96 Plates; Flat-Bottom Blocks ａnd Lids, Reagents, Buffers, Collection Microtubes (1.2 ml) ａnd Caps, 96-Well Microplates RB ａnd Lids, Tape Pads         CNY                              【人吡哆醛/吡哆醇维生素B6磷酸酶(PDXP)ELISA试剂盒现货】

960241     QIAGEN Plasmid Plus 96 BioRobot Kit (4)                  CNY                             

51304       QIAamp DNA Mini Kit (50)       For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml)     CNY                             

978756     PyroMark CpG Assay 96 well (200)           CNY                             

9012712  Z-motor (Plato 7) left                 CNY                             

9012696  Set of X-flat band cable (Plato 7)               CNY                             

133239     NeXtalStock Sodium citrate pH 7.0 (200)                  CNY                             

133238     NeXtalStock Sodium citrate pH 5.8 (200)                  CNY                             

9002127  QIAsymphony RGQ, Priority    QIAsymphony RGQ Priority includes the QIAsymphony RGQ instruments, installations ａnd extended warranties. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents. CNY                            

59203       EpiTect Whole Bisulfitome Kit (25)           CNY                             

59164       EpiTect Plus LyseAll Bisulfite Kit (48)                  CNY                             

929526     QX Alignment Marker 50 bp/1 kb (1.5 ml)                 CNY                             

133218     NeXtalStock MOPS pH 8.0 (200)                CNY                             

301605     RNAiFect Transfection Reagent (1ml)      RNAiFect Reagent ａnd buffer, for up to 170 transfections in 24-well plates; up to 500 transfections in 96-well plates  CNY                             

301525     TransMessenger Tranfect. Reagent(0.5ml)     For 60 transfections in 6-well plates or 80 transfections in 12-well plates         CNY                             

52304       QIAamp RNA Blood Mini Kit (50)     For 50 RNA preps: 50 QIAamp Mini Spin Columns, 50 QIAshredder Spin Columns, Collection Tubes (1.5 ml ａnd 2 ml), RNase-free Reagents ａnd Buffers    CNY                             

51306       QIAamp DNA Mini Kit (250)     For 250 DNA preps: 250 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml)     CNY                              【人吡哆醛/吡哆醇维生素B6磷酸酶(PDXP)ELISA试剂盒现货】

9002012  Rotor-Gene Q MDx 2plex HRM Platform                  CNY