价格详细资料：试剂盒价格电议，齐一生物销售：021－6034 8496；181214 53965；173021 04490,齐一生物科技（上海）有限公司提供ELISA试剂盒受到了广大研究所.中科院科研单位一致肯定和认同。齐一生物大品牌保证，倾力为国内外科研院校实验室提供*优质产品。

【人T细胞特异性鸟苷三磷酸酶(Tgtp)ELISA试剂盒厂家】注意事项

1. 当混合蛋白溶液时应尽量轻缓，避免起泡。

2. 洗涤过程非常重要，不充分的洗涤易造成假阳性。

3. 一次加样时间控制在5分钟内，如标本数量多，推荐使用排枪加样。

4. 请每次测定的同时做标准曲线，做复孔。

5. 如标本中待测物质含量过高，请先稀释后再测定，计算时请*后乘以稀释倍数。

6. 在配制标准品、检测溶液工作液时，请以相应的稀释液配制，不能混淆。

7. 底物请避光保存。

8. 不要用其它生产厂家的试剂替换试剂盒中的试剂。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【人T细胞特异性鸟苷三磷酸酶(Tgtp)ELISA试剂盒厂家】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【人T细胞特异性鸟苷三磷酸酶(Tgtp)ELISA试剂盒厂家】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【人T细胞特异性鸟苷三磷酸酶(Tgtp)ELISA试剂盒厂家】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

QY-18872 Anti-TRA16 (Testicular orphan nuclear receptor-4 (TR4)-associated protein)睾丸激素受体相关蛋白16抗体                                                

QY-18882 Anti-TRA16 (Testicular orphan nuclear receptor-4 (TR4)-associated protein)睾丸激素受体相关蛋白/孤儿素受体相关蛋白抗体                                                       

QY-18892 Anti-TRADD （TNF receptor 1 associated via death domain）有死亡区的肿瘤坏死因子受体1相关蛋白抗体                                                   

QY-18902 Anti-TRAF1 （TNF receptor-associated factor 1）肿瘤坏死因子受体相关因子1抗体                                                  

QY-18912 Anti-TRAF2 （TNF receptor-associated factor 2）肿瘤坏死因子受体相关因子2抗体                                                  

QY-18922 Anti-TRAF3/CD40bp （CD40 binding protein）CD40结合蛋白/CD40受体相关因子1抗体                                                   

QY-18932 Anti-TRAF6 （TNF receptor-associated factor 6）肿瘤坏死因子受体相关因子6抗体                                                  

QY-18942 Anti-TRAIL/Apo2L (tumor necrosis factor-related apoptosis-inducingligand)肿瘤坏死因子相关凋亡诱导配体/凋亡素2配体抗体                                                   【人T细胞特异性鸟苷三磷酸酶(Tgtp)ELISA试剂盒厂家】

QY-18952 Anti-Transthyretin (TTR/prealbumin)转甲状腺素蛋白抗体                                                

QY-18962 Anti-TRBF1 (Telomeric repeat binding factor 1)端粒体复制结合因子1抗体                                                    

QY-18972 Anti-TRF1（telomere repeat binding factor1）端粒结合蛋白1抗体                                               

QY-18982 Anti-TrK A (Tyrosine kinase,TrkA)酪氨酸激酶A抗体(神经生长因子受体的一种)                                                     

QY-18992 Anti-TrK A.B.C(Tyrosine kinase,TrkA.B.C)酪氨酸激酶A.B.C抗体                                                

QY-19002 Anti-Trk B(Tyrosine kinase,Pyk2)酪氨酸激酶B抗体(神经生长因子受体的一种)                                                      

QY-19012 Anti-Trk B(Tyrosine kinase,TrkB)酪氨酸激酶B抗体(神经生长因子受体的一种)                                                       

QY-19022 Anti-Trk C(Tyrosine kinase,TrkC)酪氨酸激酶C抗体(神经生长因子受体的一种)                                              

QY-19032 Anti-Trop-2Trop-2抗体                                                 

QY-19042 Anti-TSARG3（Testis ａnd spermatogenesis related gene 3）生精细胞凋亡相关基因3抗体                                                  

QY-19052 Anti-TSARG4（Testis ａnd spermatogenesis related gene 4）生精细胞凋亡相关基因4抗体                                                  

QY-19062 Anti-TSGF（Tumor Specific Growth Fanctor）恶性肿瘤特异性生长因子抗体                                                 

QY-19072 Anti-TSH(Mouse Anti-Human Thyroid stimulating hormone Monoclonal Antibody)鼠抗人促甲状腺素单克隆抗体                                                

QY-19082 Anti-TSHR (Thyroid stimulating hormone receptor)促甲状腺素受体抗体                                                  

QY-19092 Anti-TSHR (Thyroid stimulating hormone receptor)（CT）促甲状腺素受体抗体（C端）                                              

QY-19102 Anti-TSHR (Thyroid stimulating hormone receptor)(NT)促甲状腺素受体抗体（N端）                                                     【人T细胞特异性鸟苷三磷酸酶(Tgtp)ELISA试剂盒厂家】

QY-19112 Anti-TSLC1 (tumor suppressor in lung cancer-1)肺癌肿瘤阻抑基因1抗体                                                

QY-19122 Anti-TSLP(Thymic stromal lymphopoietin isoform 1)淋巴细胞生成素-1抗体                                                     

QY-19132 Anti-TTF-1 (Thyroid Transcription Factor-1)甲状腺核转录因子-1/甲状腺特异增强子结合蛋白抗体                                                        

QY-19142 Anti-TTF-2(thyroid transcription factor 2;forkhead box E1)甲状腺转录因子-2抗体                                                  

QY-19152 Anti-Tubulin-α微管蛋白抗体                                                       

QY-19162 Anti-Tubulin-β微管蛋白（免疫组化用抗体）                                                  

QY-19172 Anti-Tubulin-β微管蛋白抗体                                                       

QY-19182 Anti-Tubulin-γ微管蛋白-γ抗体                                                 

QY-19192 Anti-TY3H/TH (Tyrosine Hydroxylase )酪氨酸羟化酶抗体                                                   

970530     NRAS Pyro Kit (24)            CNY                             

970022     PyroMark Q24 CpG MLH1 (4 x 24)   For quantification of methylation level in region -209 to -188 from the transcription start site. CNY                             

969261     Ni-NTA Superflow 96 BioRobot Kit (4)               CNY                             

133199     NeXtalStock HEPES pH 8.0 (200)               CNY                             

292143     mericon Horse Kit (24)              CNY                             

206047     Type-it Fast SNP Probe PCR Kit (4000)     "Features

 Outstanding separation for reliably high call rates

 For difficult SNP loci ａnd for low amounts of template

 Validated using TaqMan SNP Genotyping Assays

 Up to 40% time savings due to fast-cycling procedure

 Convenient ｍaster mix format ａnd optimized protocol"   CNY                             

292025     mericon Cattle (96)           CNY                             

291113     mericon RR Soy Kit (24)            CNY                             

9001908  QIAgility HEPA / UV (w/o PC) System       QIAgility HEPA / UV (w/o PC) System combines the QIAgility instrument ａnd installation. The Installation plus Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document. CNY                             

133111     NeXtalStock Sodium fluoride (200)           CNY                             

9018540  Kit, installation, Qsym               CNY                             

9240672  Kerk Nut, Common, AP             CNY                             

9240658  Pump, Vacuum, Jun-Air, AP               CNY                             

9240642  Cable Assy, Proteinase K Pump, AP196/98                  CNY                             

9240638  Opto Sensor, Reagent Pump, AP               CNY                              【人T细胞特异性鸟苷三磷酸酶(Tgtp)ELISA试剂盒厂家】

9240617  Fuse, 2.0A, FNM, AP96             CNY                             

9240589  MAC Valve, 4-Way, Cent. Locator, AP96           CNY                             

9240560  Rotor/Buckets, Eppendorf, AP196/98                CNY                             

9240554  Bucket Grease, Eppendorf, AP196/98                CNY                             

9240523  Cable, Motor, Axis #0, Dispense, AP98             CNY                             

9240507  Software & Std. Protocols, AP196            CNY                             

9240377  QIAcube, Installation       The Installation, QIAcube facilitates the quick implementation of the QIACube into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the QIAcube instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.  CNY                             

9239571  BR Universal, Basic Agreement       The Basic Cover Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9239570  BR Universal, Premium Agreement         The Premium Cover Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).    CNY                             

9020032  FOOTSET, RGQ                   CNY                             

9019986  PCB, small breakout, 12 core, C12            CNY                             

9019479  UPPER BUFFER LID GS              CNY                             

9238864  BR M48, Premium Agreement         The Premium Cover Agreement M48 provides full service coverage for the BioRobot M48. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).   CNY                             

9238852  LiquiChip, Basic Agreement     The Basic Cover Agreement LiquiChip provides full service coverage for the LiquiChip workstation. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9241224  QIAxcel, Inspection Service      "Repair service delivery not applicable

Service response time (repair) not applicable

Periodic inspection/maintenance: yes, 1/agreement year

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: not included

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: Yes, included" CNY                             

9241197  Silicone sealant, RTV 108, QX           CNY                              【人T细胞特异性鸟苷三磷酸酶(Tgtp)ELISA试剂盒厂家】