【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【人基质金属蛋白酶11(MMP11)ELISA试剂盒现货】样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【人基质金属蛋白酶11(MMP11)ELISA试剂盒现货】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

【人基质金属蛋白酶11(MMP11)ELISA试剂盒现货】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【人基质金属蛋白酶11(MMP11)ELISA试剂盒现货】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

QY-00070       AM Adrenomedullin 肾上腺髓质素（抗原）             0.1mg/1800                       

QY-04160       TIMP-2(Tissue Inhibitor of Metalloproteinase-2)金属蛋白酶组织抑制因子-2(抗原)              0.5mg/6501mg/1100                           

QY-04170       TIMP-3(Tissue Inhibitor of Metalloproteinase-3)金属蛋白酶组织抑制因子(抗原)          0.5mg/6501mg/1100                         

QY-00900       ADM R, Adrenomedullin receptor肾上腺髓质素受体（抗原）         0.5mg/6501mg/1100                         

Yf-01580 Amylin1-37/FITC异硫氰酸荧光素(FITC)标记糖尿病相关肽1-37          1ml/2860                           

QY-03060       VTG(vitellogenin)青鳉鱼卵黄蛋白              0.1mg/6500.2mg/1100                      

QY-00580       Apaf-1 （Apoptosis protease activating factor-1）(CT)凋亡蛋白活性因子-1（抗原）          0.5mg/7001mg/1200                            【人基质金属蛋白酶11(MMP11)ELISA试剂盒现货】

QY-00590       Apaf-1 （Apoptosis protease activating factor-1）(NT)凋亡蛋白活性因子-1（抗原）          0.5mg/7001mg/1200                           

QY-01180       AR （Androgen Receptor)雄激素受体（抗原）               0.5mg/6501mg/1100                         

QY-00450       AT （Angiotenogen）血管紧张素原（抗原）           0.5mg/6501mg/1100                         

QY-01670       Apo-E （Apolipoprotein E）载脂蛋白E（抗原）             0.5mg/6501mg/1100                         

QY-02450       IKI3 family protein         0.5mg/8001mg/1200                         

QY-03170       ILK-1 (Integrin-linked protein kinase 1)整合素连接激酶-1         0.5mg/6501mg/1100                         

QY-01140       Aromatase芳香化酶（抗原）             0.5mg/6501mg/1100                         

QY-00550       ART(Artemin)（抗原）         0.5mg/6501mg/1100                         

QY-02610       AQP-2 （aquaporin Protein-2）水通道蛋白-2（抗原）           0.5mg/6501mg/1100                         

QY-01640       BACE( ASP2 ） β分泌酶（抗原）           0.5mg/6501mg/1100                         

QY-02750       Bassoon(RCN)               0.5mg/6501mg/1200                         

QY-01270       Bax （Bcl-2 Assaciated X protein） Bax（多肽抗原）             0.5mg/6501mg/1100                         

QY-00320       Bcl-2（抗原）Bcl-2（多肽抗原）              0.5mg/6501mg/1100                         

QY-02480       BDNF (Brain-derived Neurotrophin factor)脑源神经营养因子(脑衍化神经营养因子)            0.5mg/6501mg/1100                           

QY-02170       bFGF碱性成纤维细胞生长因子（多肽抗原）          0.5mg/6501mg/1100                         

QY-00440       BNP （Brain Natriuretic Peptide ）脑钠素（多肽抗原）         0.5mg/6501mg/1100                          【人基质金属蛋白酶11(MMP11)ELISA试剂盒现货】

QY-04890       BrdU (Bromodeoxyuridine)溴脱氧尿苷               50mg/100100mg/1501g/1300                          

QY-03240       C5过敏毒素C5（补体C5）抗原               0.5mg/6501mg/1100                         

QY-00910       CA125 （Ovarian cancer antigen）卵巢癌抗原（抗原）          0.5mg/6501mg/1100                         

QY-00430       CAD（Caspase-Activated Deoxyribonuclease）Caspase 激活的脱氧核糖核酸酶（抗原）           0.5mg/6501mg/1100                         

QY-00950       Calponin 1钙调节蛋白-1（抗原）              0.5mg/6501mg/1100                         

QY-01690       Caspase-1 (ICE; CASP-1；P45)天冬氨酸-胱氨酸特异性蛋白酶家族（抗原）             0.5mg/6501mg/1100                         

QY-00810       Caspase-3 （CPP32）半胱胺酸蛋白酶蛋白-3（抗原）           0.5mg/6501mg/1100                         

QY-00840       Caspase-6 （NT）半胱胺酸蛋白酶蛋白-6（抗原）         0.5mg/6501mg/1100                         

QY-01510       Caspase-6 （CT）半胱胺酸蛋白酶蛋白-6（抗原）         0.5mg/6501mg/1100                         

QY-00490       Caspase-9白介素1-β转化酶样凋亡蛋白酶6（抗原）           0.5mg/6501mg/1100                         

9001946  QIAcube (220-240V) Promo        CNY                           

971061    therascreen® MGMT Pyro Kit (48)             CNY                           

293035    mericon Apricot kernels (96)        CNY                           

929520    QX Alignment Marker 15bp/500bp (1.5 ml)         CNY                           

9012643  Bottle, system liquid              CNY                           

9012596  Disposable Tips, 300µl (960) Conducting disposable tips    CNY                           

133121    NeXtalStock Lithium citrate  (200)            CNY                           

133119    NeXtalStock Ammonium citrate  (200)              CNY                           

133190    NeXtalStock D-(+)-Glucose (200)              CNY                           

51162      QIAamp 96 DNA Blood Kit (12)    For 12 x 96 DNA preps: 12 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels  CNY                           

50112      QIAamp UCP PurePathogen Blood Kit (10)         CNY                           

133104    NeXtalStock Sodium acet.  (200)              CNY                           

9015468  Sensor, photo, PM-L24         CNY                           

9001871  EZ1 Advanced, System  EZ1 Advanced, System combines the EZ1 Advanced system ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with a training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.    CNY                           

9018458  Screw, w finishing washer (4), QX             CNY                           

9018447  Purge Tool, cartridge, QX            CNY                            【人基质金属蛋白酶11(MMP11)ELISA试剂盒现货】

9012412  Incubator Tower (65°) for Plato 3 hold        CNY                           

9012392  Bottle, waste, 10 l, BR8000          CNY                           

205111    Omniscript RT Kit (50)  For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free water      CNY                           

9018425  Door, sample, QX         CNY                           

9002123  QIAxcel Advanced System    QIAxcel Advanced System combines the QIAxcel Advanced instrument ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.    CNY                           

57714     QIAamp MinElute Virus Vacuum Kit (50)     For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, Carrier RNA, Buffers, Extension Tubes (3 ml), Collection Tubes (1.5 ml)     CNY                           

972012    PyroMark Q96 CpG p16 (96)       For quantification of methylation level in p16       CNY                           

133227    NeXtalStock Sodium cacodyl. pH 6.0 (100)        CNY                           

301108    PolyFect Transfection Reagent (100 ml)      For 2500-6500 transfections in 60 mm dishes or 5000-10,000 transfections in 6-well plates.  CNY                           

56304     QIAamp DNA Micro Kit (50) For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Carrier RNA, Buffers, Collection Tubes (2 ml)  CNY                           

9002031  Rotor-Gene Q MDx 5Plex HRM no PC        CNY                           

9002018  Rotor-Gene Q MDx 2plex HRM, Priority     Rotor-Gene Q 2plex HRM, Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents. CNY                           

970902    PyroMark Q24 Advanced Kit (4x24)            CNY                           

133211    NeXtalStock Magnesium chloride H2O (200)             CNY                           

133195    NeXtalStock Sodium tartrate pH 5.6 (200)          CNY