检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供*优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【人酪氨酸激酶2(Tyk-2)ELISA试剂盒厂家】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人酪氨酸激酶2(Tyk-2)ELISA试剂盒厂家】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在、第二孔中分别加标准品100μl,然后在、第二孔中加标准品稀释液50μl,混匀；然后从孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【人酪氨酸激酶2(Tyk-2)ELISA试剂盒厂家】

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品*终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果. 【人酪氨酸激酶2(Tyk-2)ELISA试剂盒厂家】

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请*后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

QY-07700 SP-A (pulmonary surfactant-associated glycoprotein A)肺表面活性蛋白A(抗原)                   0.5mg/6501mg/1100                                  

QY-06790 SYVN1(synovial apoptosis inhibitor 1)滑膜细胞凋亡抑制物1（抗体）                  0.5mg/6501mg/1100                                  

QY-06850 SYK (Spleen tyrosine kinase)非受体型酪氨酸激酶(抗原)                 0.5mg/6501mg/1100                                  

QY-04190 Tau protein微管相关蛋白（抗原）                0.5mg/6501mg/1100                                  

QY-06380 TGF-βR 1/ALK-5(TGF-beta receptor type I)转移生长因子–β受体 1（抗原）                   0.5mg/6501mg/1100                                  

QY-07780 Thy-1/CD90CD90(抗原)              0.5mg/6501mg/1100                                  

QY-06320 Thioster-containing protein 1硫酯包含蛋白-1(抗原)              0.5mg/6501mg/1100                                  

QY-06400 TIGAR humen (TP53- induced glycolysis ａnd apoptosis-regulator )P53诱导糖酵解和凋亡调节因子蛋白（抗原）            0.5mg/6501mg/1100                                   【人酪氨酸激酶2(Tyk-2)ELISA试剂盒厂家】

QY-04180 TIMP-4(Tissue Inhibitor of Metalloproteinase-4)金属蛋白酶组织抑制因子-4(抗原)             0.5mg/6501mg/1100                                  

QY-04470 TPO(Thyroid Peroxidase)甲状腺过氧化物酶(抗原)                  0.5mg/6501mg/1100                                  

QY-04600 TSHR (Thyroid stimulating hormone receptor)促甲状腺素受体（抗原）              0.5mg/6501mg/1100                                  

QY-04490 TSLP(Thymic stromal lymphopoietin isoform 1)淋巴细胞生成素-1（抗原）                   0.5mg/6501mg/1100                                 

QY-04460 TTF-2(thyroid transcription factor 2;forkhead box E1)甲状腺转录因子-2(抗原)             0.5ml/6501mg/1100                                   

QY-08190 Tyrosinase酪氨酸酶(多肽抗原)               0.5mg/6501mg/1100                                  

QY-06030 OAT4L又称URAT1 (Urate Transporter 1)尿酸盐重吸收转运子1 (抗原)               0.5mg/6501mg/1100                                  

QY-08530 VAP1(vascular adhesion protein 1)血管粘附蛋白1（多肽抗原）                  0.5mg/6501mg/1100                                  

QY-06590 VCAM-1(Vascuolar cell adhesion molecule 1)CD106血管内皮细胞粘附分子(抗原)               0.5mg/6501mg/1100                                  

QY-07560 Vimentin波形蛋白（抗原）                    0.5mg/6501mg/1100                                  

QY-04790 VSIG4 (V-set ａnd immunoglobulin domain-containing protein 4)VSIG4 T淋巴细胞负调节蛋白（抗原）            0.5mg/6501mg/1100                                  

QY-04290 WNK4(Ser/Thr-protein kinase WNK4; protein kinase with no lysine 4; protein kinase, lysine –dficient 4)一种新的丝氨酸/苏氨酸激酶家族成员的基因WNK4（多肽抗原）                0.5mg/6501mg/1100                                  

QY-06280 ZNF300 (zinc finger protein ZNF300)- middle锌指蛋白300(抗原)                   0.5ml/6501mg/1100                                   

QY-0625R          ZNF268 (zinc finger protein ZNF268)- N-terminal (1a)锌指脂蛋白-1a(抗原)                    0.5mg/6501mg/1100                                  

QY-06260 ZNF268 (zinc finger protein ZNF268)- middle (1b)锌指脂蛋白-1b(抗原）               0.5mg/6501mg/1100                                  

QY-06270 ZNF268 (zinc finger protein ZNF268)- middle (1c)人锌指脂蛋白-1c(抗原)              0.5mg/6501mg/1100                                  【人酪氨酸激酶2(Tyk-2)ELISA试剂盒厂家】

QY-04800 IFN-γ(Interferon γ)Ag-mouse、rat干扰素-γ多肽抗原（大、小鼠）            0.5mg/6501mg/1100                                  

QY-04810 IFN-γ(Interferon γ)Ag-Human干扰素-γ多肽抗原（人）                 0.5mg/6501mg/1100                                  

QY-07840 IFN-β (Interferon β)Anti-mouse,rat干扰素-β抗原                    0.5mg/6501mg/1100                                  

QY-07870 IFN-β (Interferon β)Anti-human干扰素-β抗原                 0.5mg/6501mg/1100                                  

QY-06690 PSCA (Prostate Stem Cell Antigen) 前列腺干细胞抗原                    0.5mg/6501mg/1100                                  

QY-06450 sIgA大鼠分泌型IgA(抗原)               0.5mg/6501mg/1100                                  

QY-09040 Melamine/KLH三聚氰胺与血蓝蛋白偶联物                   5mg/200010mg/3000                                 

QY-09041 Melamine/RCA三聚氰胺与牛血清白蛋白偶联物                  2mg/7805mg/150010mg/2500                                   

QY-09042 Rabbit IgG Sepharose 4B兔IgG亲和层析柱                    2ml柱床体积/15005ml柱床体积/2500                                    

133187     NeXtalStock Citric acid pH 5.0 (200)                  CNY                             

51126       QIAamp DNA Blood Mini QIAcube Kit (240)             CNY                             

133096     NeXtalStock Potassium phosphate (200)          CNY                             

133095     NeXtalStock Potassium chloride (200)               CNY                             

959034     EZ1 RNA Tissue Mini Kit (48)   48 Reagent Cartridges (RNA Tissue) 100 Disposable Tip Holders, 100 Disposable Filter-Tips, 50 Sample Tubes (2 ml), 50 Elution Tubes (1.5 ml), Buffer RLT, RNase-Free DNase I       CNY                             

957336     MagAttract Direct mRNA M48 Kit (192)                   CNY                             

9018448  O-ring, purge port, QX              CNY                             

133172     NeXtalStock CHES pH 8.5 (200)                 CNY                             

133171     NeXtalStock CAPSO pH 9.8 (200)               CNY                             

9018408  Regulator, N2, rear, QX            CNY                             

291045     mericon Screen nos Kit (96)              CNY                             

291025     mericon GMO Screen 35S-pat  (96)                 CNY                             

291013     mericon Screen 35S Kit (24)              CNY                             

9001866  Rotor-Gene Q 5plex (PC) PrioPLUS  "Rotor-Gene Instrument

Installation of Rotor-Gene & Introductory training

Warranty extension to 3 yrs

Loaner available in 2 days*

Annual Inspections (1 onsite PM per yearr)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

9012326  Set Peristaltic Pump Tubing Plato 3000            CNY                              【人酪氨酸激酶2(Tyk-2)ELISA试剂盒厂家】

9012322  Tubing set, peristaltic pump, 1x15 cm              CNY                             

1070195  VacValve Tool (1)               CNY                             

1067933  Buffer RWT (concentrate, 80 ml)              CNY                             

133080     NeXtalStock PEG 20,000 (200)                   CNY                             

38071       AIL Cartridge (1ml)           CNY                             

38015       #WGA Cartridge (5ml)               CNY                             

204754     QuantiFast Multiplex PCR +R Kit (400)              CNY                             

204643     QuantiTect Multiplex RT-PCR Kit (200)             CNY                             

204574     Rotor-Gene Probe RT-PCR Kit (400)        The Rotor-Gene Probe RT-PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly sensitive quantification in real-time one-step RT-PCR using sequence-specific probes.         CNY                            

204363     QuantiTect Probe PCR + UNG Kit (200)   The supplied, ready-to-use ｍaster mix contains HotStarTaq DNA Polymerase, ROX passive reference dye, ａnd a dNTP mix in an optimized buffer. The dNTP mix includes dUTP, allowing optional treatment with UNG. The UNG enzyme is provided separately. For convenience, the ｍaster mix can be stored at 2–8°C.     CNY                             

9015350  Board, terminal                 CNY                             

9015349  Board, motor controller           CNY                             

9018402  Kit, purge filter, QX           CNY                             

9018389  QIAcube Tip Adapter Ring Replacement Kit            CNY                             

9012283  Power Supply, 6 amp for P 90, (7135)                CNY                             

9012180  Syringe pump inlet tubing                  CNY                              【人酪氨酸激酶2(Tyk-2)ELISA试剂盒厂家】