1.购买我公司任意ELISA试剂盒指标享受免费代测，并提供技术指导服务！

二、实验材料与试剂配制：

1. 仪器与材料：酶标仪（使用前预热30分钟），微量加液器、吸头、蒸馏水或去离子水，滤纸。

2. 缓冲液使用：加5ul  的缓冲液于50ul  的样品中，如果样品量不够或者不确定，缓冲液和样品的混合比例不要小于1:10即可混匀，静置1小时备用（如果标本是血清或者血浆，此步骤忽略）。

3. 洗液的配制：按1:100的比例配制洗液备用。

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA试剂盒厂家】三. 仔细阅读说明书

1.检查试剂盒标签上的有效期。如果超过有效期，请勿使用。

2. 按说明书确定所有试剂齐全（数量、体积）

3. 标本制备要规范，每份标本体积要按2-3个复孔以上的量制备（贮存），尽量分装做备份。

4.短期内无法实验者，注意低温保存

5.准备好所有实验额外所需物品，（比如移液器，试管，清洗器，酶标仪）

6. 按说明书将所用试剂平衡至室温，根据检测标本数量确定所需试剂的量

7.检查试剂盒内每种试剂的贮存条件，保证所有试剂均按照试剂盒内说明书推荐的条件存储。 【人N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA试剂盒厂家】

8.检查不稳定或者变质的试剂溶液（比如沉淀或变色）。有些试剂，比如标准品稀释液或者检测稀释液，可能在设计时就含有沉淀物。所有试剂在滴入酶标板之前，必需充分混匀。而由于沉淀物的特性，在加入酶标板之前，必需不停搅拌。

9.保证充分的孵育时间和温度。

10.切勿使用不同生产批号的试剂取代现有试剂或混合现有试剂。

11.在混合或溶解蛋白溶液时，避免泡沫产生。

12.在实验开始之前，安排好实验流程。在实验开始之前，清洁工作台。

13.若有问题，应与我公司或代理商的技术支持联系

【人N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA试剂盒厂家】四、客户须知

齐一生物销售：021－6034 8496；181214 53965；173021 04490

1.在收集标本前都必须有一个完整的计划，必须清楚要检测的成份是否足够稳定。我们提倡新鲜标本尽早检测，对收集后当天就进行检测的标本，及时储存在4℃备用，如有特殊原因需要周期收集标本，请造模取材后，将标本及时分装后放在-20℃或-70℃条件下保存。因冰室与室温存在一定温差，蛋白极易降解，直接影响实验质量，所以避免反复冻融。部分标本也可加入适当防腐剂，激素类标本需添加抑肽酶。 

2.试剂盒代测标本的客户取材前须向我司销售人员索要说明书，具体操作注意事项请与我司技术人员沟通。

3.液体类标本：标本必须为液体，不含沉淀。包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清、组织匀浆等。

4.购买前请提前告诉我们：您样本的种属、样本数量、待测指标及其它特殊要求。

【人N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA试剂盒厂家】五、代测数据详细内容

1、ELISA标准曲线；

2、实验具体详细步骤；

3、完整的实验报告（试剂耗材，实验方法，实验结果及结果说明）；

4、实验进行阶段，我公司客服人员会及时向您汇报实验进程。

9238858  Basic Cover Agreement Plato7, 1yr          The Basic Cover Agreement Plato7 provides full service coverage for the Plato 7 platform. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9241233  User Manual, QIAxcel FA 01/08 (WW)                CNY                             

9241227  ZLV, Handscanner, Qsym          CNY                             

9241180  Motor, Z, QX             CNY                             

9019473  Loading guide, 52 well sharkstooth, GSC                   CNY                             

9019462  Switch, micro, HS              CNY                             

9019457  Fans (2), 5V, 30mm, HS             CNY                             

9237172  Cable USB                  CNY                             

9233169  Board, YZ-CPU, BR8000            CNY                              【人N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA试剂盒厂家】

9237163  Bezel LiquiChip                   CNY                             

9237162  Shipping Plug XYP              CNY                             

9237135  #Twister I, Full Agreement, no PM The Full Agreement BR Twister I provides full service coverage for the BioRobot Twister I. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Full Agreement, there is a priority response time of 48 hours (2 working days).       CNY                             

9237109  Protocol Dev./Opt., on-site (5-days)         The QIAGEN BioRobot Systems Protocol Development/Optimization On-Site Visit includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Instrumentation Service Specialist to write and/or opimize protocols for the BioRobot System. Parts, consumable chemistries, ａnd accessories not included. Custom protocols are the sole responsibility of the customer ａnd neither warrantied nor guaranteed.          CNY                             

9237102  Upgrade, BR plus 2 ext. devices       Upgrade BioRobot with two approved integration devices    CNY                             

9232974  Card, x-main, peristaltic XPUPG                CNY                             

9232972  YZ 96 CPU Board/Driver Unit            CNY                             

9232947  Springcontact, RoboVac, BR8000             CNY                             

9236408  Storage Platform TW I, deep well             CNY                             

9232918  Board, PCON, auto vac, BR8000               CNY                             

9236334  Green LSR                  CNY                             

9236324  Fan, LiquiChip            CNY                             

9236151  Locator Plate, 96 well,pos 2,BR RP          CNY                             

9230820  Rack for reagents, Universal (zig-zag)              CNY                             

9230718  Predilution-Rack 320x2ml Rose-80           CNY                             

9230365  Cable,Interface,flat,(Syr.)-Incub.-Read.            CNY                              【人N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA试剂盒厂家】

9021284  Safety lock, waste drawer, v1, Qsym                 CNY                             

9238611  Rubber seal, centrifuge motor                  CNY                             

9238477  Tubing, OLF pump head, RapidPlate                  CNY                             

9238245  Tray for Reaction               CNY                             

9238191  Upgrade App., DirectPrep 96           CNY                             

9237721  EZ1, Full Agreement         The Full Agreement EZ1 provides full service coverage for the BioRobot EZ1. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).   CNY                            

9240676  Bimba Cylinder, Latch Lock, AP98             CNY                             

9240668  Nut, Flangeless,1/4-28, Blue, AP                CNY                             

QY-x1014 人肝癌细胞HepG2/2-15                                              

QY-x1015 人肝癌细胞9204                                                  【人N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA试剂盒厂家】

QY-x1016 人肝癌细胞HuH-7                                               

QY-x1017 人肝癌细胞FL62891                                                    

QY-x1018 人胆管细胞型肝癌细胞HCCC-9810                                                    

QY-x1019 人肝癌细胞HCC-9724                                                  

QY-x1020 人肝癌细胞HHCC                                                

QY-x1021 人肝癌细胞系Hep-3B                                                 

QY-x1022 表达HBV病毒的人肝细胞HepG2.2.15                                                      

QY-x1023 人肝癌细胞H7402                                              

QY-x1024 人胚肝二倍体细胞CCC-HEL-1                                                     

QY-x1025 人肝细胞FL62891                                                

QY-x1026 人感染HCV肝癌细胞HuH7-HCV                                               

QY-x1027 永生化鼻咽上皮细胞系NP69-SV40T                                                  

QY-x1028 人鼻咽癌母系细胞CNE-2Z                                                  

QY-x1029 人鼻咽癌细胞CNE                                              

QY-x1030 人鼻咽癌细胞（高分化）CNE1                                                  

QY-x1031 人鼻咽癌细胞（低分化）CNE2                                                   【人N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA试剂盒厂家】

QY-x1032 人鼻咽癌细胞HNE1                                                     

QY-x1033 人鼻咽癌细胞HNE2                                                     

QY-x1034 人鼻咽癌细胞HNE3                                                     

QY-x1035 人鼻咽癌细胞HONE1                                                  

QY-x1036 转基因鼻咽癌细胞系CNE1-lmp\l                                               

QY-x1037 低分化鼻咽癌细胞系SUNE-1                                                      

QY-x1038 鼻咽癌细胞系SUNE-1亚株-5-8F                                                

QY-x1039 5-8转基因鼻咽癌细胞系F2-2                                                     

QY-x1040 人鼻咽癌细胞系SUNE-1亚株6-10B                                                  

QY-x1041 人鼻咽癌细胞系SUNE-1亚株13-9B                                                  

QY-x1042 人鼻咽癌细胞系HNE2-LMP1/2                                                    

QY-x1043 人鼻咽癌细胞系HNE2-LMP1                                                       

QY-x1044 人鼻咽癌细胞系SUME-a