【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【人组织蛋白酶D(cath-D)ELISA试剂盒现货】样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【人组织蛋白酶D(cath-D)ELISA试剂盒现货】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

【人组织蛋白酶D(cath-D)ELISA试剂盒现货】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【人组织蛋白酶D(cath-D)ELISA试剂盒现货】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

286115    cador N. caninum PCR Reagent (96)           CNY                           

282603    virotype Influenza A RT-PCR Kit (24)          CNY                           

282607    virotype Influenza A RT-PCR Kit (480)        CNY                           

280373    virotype BVDV RT-PCR Kit (24)          CNY                           

280445    virotype BTV pan/8 RT-PCR Kit (96)           CNY                           

9018703  EZ1 Adv. XL DSP Virus Card        CNY                           

9001252  BIOROBOT 8000 Platf.Promo,Reaction Setup            CNY                           

9019515  ZLV Y-piece, pinch valve tubing, CX1          CNY                           

9020575  LED Assy, Green, 15" Long, DML 3000             CNY                           

9020593  Lid, DML3000              CNY                            【人组织蛋白酶D(cath-D)ELISA试剂盒现货】

9022383  Original Transport Box, QXA              CNY                           

9013308  Base, vacuum manifold, v1.04             CNY                           

9013298  Top plate, vacuum manifold96 plate , 4        CNY                           

9013236  Tubing set, vacuum, 200, 0          CNY                           

9013224  Gripper set, V-handler, BR3000            CNY                           

9013197  Tubing, L1 outlet, auto vac,BR8000 v1.00          CNY                           

9011702  Cable, PCON98 to AC-box (level sensor),           CNY                           

9011681  Dispenser drive, BR8000              CNY                           

132635    NeXtal Stock Zinc chloride (50)    0,1M Zinc chloride - 50mL (4 x 12,5 ml tube)     CNY                           

132578    NeXtal Stock PEG 2,000 DME (50)     50% w/v Polyethylene glycol 2,000 dimethyl ether - 50mL (4 x 12,5 ml tube)       CNY                           

800341    Cosmid End-Sequencing, w/ prep        CNY                           

800308    siRNA Hairpin Run w/ prep          CNY                           

800121    Verification Sequencing, w/ prep   "Double-stranded sequencing of a given sequence

QIAGEN® DNA template preparation

Design ａnd synthesis of walking primers

Data assembly ａnd sequence alignment

Accuracy >99.99%"      CNY                           

132528    NeXtal Stock CAPSO untit. (50)   0.5M CAPSO - Untitrated - 50mL (4 x 12,5 ml tube)   CNY                           

132510    NeXtal Stock ADA untit. (50)       0.5M ADA - Untitrated - 50mL (4 x 12,5 ml tube)       CNY                           

764114    PAXgene Bone Marrow RNA Tubes (50)            CNY                            【人组织蛋白酶D(cath-D)ELISA试剂盒现货】

679913    ipsogen RT Kit (33)              CNY                           

203643    HotStarTaq Plus Master Mix Kit (250)  "For 250 x 20 µl reactions: 3 x 0.85 ml HotStarTaq Plus Master Mix, containing 250 units of HotStarTaq Plus DNA Polymerase total, 1 x 0.55 ml CoralLoad Concentrate, 2 x 1.9 ml RNase-free Water

HotStarTaq Plus Master Mix Kits offer all the benefits of great specificity, sensitivity, ａnd performance as HotStarTaq Plus DNA Polymerase NOW in a ｍaster mix format, which offers even faster results ａnd added convenience."     CNY                           

201225    Taq PCR Core Kit (1000)      "4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2, dNTP Mix†

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."  CNY                           

201203    Taq DNA Polymerase (250)   "250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."  CNY                           

180922    GeneRead Adapter L Set 1plex (12)             CNY                           

180912    GeneRead Adapter I Set 1plex (12)              CNY                           

159214    QIAcard FTA Indicator Four Spots (25)             CNY                           

QY-x1938       ZR-75-1 乳腺导管癌 SF767 脑瘤                                       【人组织蛋白酶D(cath-D)ELISA试剂盒现货】

QY-x1939       A10大鼠主动脉平滑肌                                         

QY-x1940       人主动脉平滑肌　CNE-1 高分化鼻咽鳞癌 1640+10%胎牛血清                                    

QY-x1941       CNE-2 低分化鼻咽鳞癌 1640+10%胎牛血清                                     

QY-x1942       KB 口腔表皮样癌 1640+10%胎牛血清                                       

QY-x1943       Hep-2 喉癌 1640+10%胎牛血清                                   

QY-x1944       Ec109 食管癌 1640+10%胎牛血清                                      

QY-x1945       KYSE 450 食管癌 1640+10%胎牛血清                                       

QY-x1946       KYSE 150 食管鳞癌 1640+10%胎牛血清                                   

QY-x1947       EC97076 食管癌 1640+10%胎牛血清                                         

QY-x1948       BGC-823 胃腺癌 1640+10%胎牛血清                                        

QY-x1949       MGC-803 胃腺癌 1640+10%胎牛血清                                       

QY-x1950       SGC7901 胃低分化腺癌 1640+10%胎牛血清(P53基因第六外显子204位密码发生缺失突变)                                   

QY-x1951       MKN-45 低分化胃癌 1640+10%胎牛血清                                         

QY-x1952       HuTu-80 十二指肠腺癌 MEM+非必需氨基酸+10%胎牛血清                                         

QY-x1953       Caco-2 结肠癌 MEM+非必需氨基酸+10%胎牛血清                                        

QY-x1954       COLO 205 结肠癌 1640+10%胎牛血清                                       【人组织蛋白酶D(cath-D)ELISA试剂盒现货】

QY-x1955       COLO320DM 结肠癌 1640+10%胎牛血清                                         

QY-x1956       HCT-8 回盲肠腺癌 1640+10%胎牛血清                                     

QY-x1957       HCT-116 低分化结肠腺癌 1640+10%胎牛血清                                         

QY-x1958       HT-29 结肠腺癌 1640+10%胎牛血清                                         

QY-x1959       LS-174T 结肠腺癌 MEM+非必需氨基酸+10%胎牛血清                                         

QY-x1960       SW480 结肠癌 L15                                      

QY-x1961       BEL-7402 肝细胞癌 1640+10%胎牛血清                                    

QY-x1962       BEL-7405 肝细胞癌 1640+10%胎牛血清                                    

QY-x1963       H7402 肝癌 1640+10%胎牛血清                                         

QY-x1964       HCC-9204 肝癌 1640+10%胎牛血清                                   

QY-x1965       Hep3B 肝癌 DMEM+非必需氨基酸+10%胎牛血清                                   

QY-x1966       Hep G2 肝癌 DMEM+10%胎牛血清                                    

QY-x1967       SMMC-7721 肝癌 1640+10%胎牛血清                                       

QY-x1968       PANC-1 胰腺导管上皮癌 1640+10%胎牛血清