1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！【人胰激肽原酶(PK)ELISA试剂盒现货】

ELISA应用：

ELISA检测试剂盒、ELISAKit、酶联免疫试剂盒、酶联免疫吸附测定试剂盒、酶联免疫分析试剂盒、酶免试剂盒等,比较常见的叫法是ELISA检测试剂盒、酶联免疫吸附测定试剂盒等ELISA试剂盒自从60-70年代问世以来,得到全世界科研工作者的认可及推崇,在欧美及中国获得很大的推广,尤其是国内生化领域的长足发展.Elisa生物试验是一种敏感性高,特异性强,重复性好的实验诊断方法.由于其试剂稳定、易保存,操作简便,结果判断较客观等因素,已广泛应用在免疫学检验的各领域中.ELISA检测试剂盒适用于体外定性检测人血清或血浆中的抗人类戊型肝炎（HEV）病毒IgM抗体ELISA检测

【人胰激肽原酶(PK)ELISA试剂盒现货】齐一生物销售：021－6034 8496；181214 53965；173021 04490  web：www.qiyibio.com

试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【人胰激肽原酶(PK)ELISA试剂盒现货】ELISA实验原理

试剂盒是固相夹心法酶联免疫吸附实验（ELISA）.已知浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测.先将生物素标记的抗体同时温育.洗涤后,加入亲和素标记过的HRP.再经过温育和洗涤,去除未结合的酶结合物,然后加入底物A、B,和酶结合物同时作用.产生颜色.颜色的深浅和样品中的浓度呈比例关系.

免疫测定技术的基础在于抗原抗体之间的特异结合反应,所以任何优质的诊断试剂离不开优质的原料,如抗原抗体,酶等.以前用于免疫测定的抗原通常为各种纯化抗原,而抗体则为纯化抗原免疫动物后获得的多克隆抗体和使用杂交瘤技术得到的单克隆抗体,而抗原或抗体的标记物如酶标结合物则通过各种化学合成方法制备.近年来,随着分子生物学的发展,使用基因工程方法制备各种特殊的抗原或抗体及其酶结合物等免疫测定试剂几成为现实的新一代试剂,各种新型使用的测定方法也不断出现.

HBsAg试剂特点（检测模式：临床抗体夹心法）：包被抗体为山羊多抗.多抗具有高亲和性和对抗原各种表位的反应性.酶表抗体为与HBsAg有不同结合位点的鼠复合单位,可测得HBsAg变异样品,提高检测的特异性（99.98%）提高检测的灵敏度,应用ParlEhrich学说（PEI）HBsAg标准品,对ad标准品的灵敏度为0.05ng/ml对ay标准品灵敏度为0.025ng/ml

ELISA检测试剂盒应用定性夹心免疫检测技术,用合成的HEV多肽抗原包被微孔板板条,这些多肽是中国型HEV毒株核心氨基酸序列中抗原性很强的肽段,分别来自于该毒株的开放阅读框2和开放阅读框3.将样品或标准品加入孔中并孵育,如果其中存在HEVIgM抗体,这些抗体就会与HEV的多肽抗原结合,并固定在上面,洗板除去其它非特异性抗体和样品中的其它成份.然后加入羊抗人IgM-HRP（辣根过氧化物酶）酶结合物,经第二次孵育后,酶结合物就会与次孵育结合上的HEVIgM抗体相结合,洗板除去未结合的酶结合物,加入TMB底物溶液,在第三次孵育时会发生酶-底物反应,只有那些含有HEVIgM抗体和酶结合物所形成的复合物的孔才会发生颜色变化,加入硫酸溶液终止酶和底物间的反应,并在波长:450nm处测量O.D.值,按照本HEVIgM抗体elisa试剂盒的测试标准,O.D.值大于或等于Cut-Off值的样品被认为是初试阳性.

以上资料仅供参考了解，不供于论文写作

【人胰激肽原酶(PK)ELISA试剂盒现货】齐一生物专业为您提供高品质，高灵敏度.高质量的酶联免疫试剂盒，齐一生物长期经营ELISA试剂盒（免费代测）及抗体、分子学生物试剂盒、金标法检测试剂盒、比色法检测试剂盒、细胞菌株、培养基、细胞因子、生化试剂、耗材等生物试剂产品。齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

9240740 Tubing, Tygon, 1/4" ID      CNY            

9240735 Tubing, Black, Capper, AP196/98     CNY            

9240724 Tube Assy, Draw, AP96/196       CNY            

5000-1412   Poteneiometer 500K, VTX2        CNY            

9240506 Monitor, 17", Flat Panel, AP        CNY            

9239851 Cover, LC200        CNY            

9239565 xMAP Alignment Microspheres     CNY            

9019942 Barrel removal tool, CX1        CNY             【人胰激肽原酶(PK)ELISA试剂盒现货】

9019474 Lid, UV, polycarb, C1x      CNY            

9238884 Stepper motor, turntable, BS96      CNY            

9238866 BR MDx, Premium Agreement   The Premium Cover Agreement MDx provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).  CNY            

9238857 Basic Cover Agreement Plato3, 1yr   The Basic Cover Agreement Plato3 provides full service coverage for the Plato3 platform. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.   CNY            

9238838 #Twister I, Basic Agreement, no PM  The Basic Cover Agreement BR Twister I provides full service coverage for the BioRobot Twister I. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Basic Cover Agreement, the response time is 5 working days.   CNY            

9241220 QIAxcel, Installation   "The Installation, QIAxcel is an on-site service, conducted by a QIAGEN Service Specialist. During the installation, the QIAxcel hardware is set up according to the manufacturer’s instructions.

This installation covers the handling of the QIAxcel systems hardware ａnd familiarize with the basic instrument ａnd software functions of the BioCalculator software. All travel ａnd labor costs ａnd well as material for the demonstration run are included. The service is documented in accordance with GMP/GLP requirements." CNY            

9241195 Cable, RS232, Belkin F2N209-06-T, 1.8m      CNY            

9241186 Sensor, interlock2, with cable, QX      CNY            

9238831 BR 8000, Basic Agreement    The Basic Cover Agreement 8000 provides full service coverage for the BioRobot 8000. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.   CNY            

9238822 Rect. carrier for 1 tube 100 ml     CNY            

9237194 Reservoir RapidPlate        CNY            

9237171 Heater Block XYP        CNY            

9233168 Mixer Board     CNY            

9237154 Drive Mechanism XYP     CNY            

9237110 Upgrade App., QIAamp 96 DNA Blood   On-site application Upgrade for standard QIAGEN QIAamp 96 DNA Blood including kit   CNY            

9237095 Training, HiLight On-site   On-site Training 1 day HiLight  CNY             【人胰激肽原酶(PK)ELISA试剂盒现货】

9237084 Installation, BR plus 3 integrations        CNY            

9236414 Plate Stacker TW II     CNY            

9232973 Level Detector Board P7-8 channels      CNY            

9232954 LSW Board (Level sensor waste)      CNY            

9232921 Comparator board 1.02 w/clot detection      CNY            

9236337 Digital Board       CNY            

9236155 Tool, calibration, syringe, RapidPlate      CNY            

9235702 Cables, Shaker, RCS1        CNY            

9235626 Lead Screw Nut, MP Holder LH, Shaker8000        CNY            

QY-x2000    U266 骨髓瘤 1640+10%胎牛血清                       

QY-x2001    BIU87 膀胱癌 1640+10%胎牛血清                      

QY-x2002    SHG-44 脑恶性胶质瘤 1640+10%胎牛血清                        【人胰激肽原酶(PK)ELISA试剂盒现货】

QY-x2003    U251 神经胶质细胞瘤 1640+10%胎牛血清                       

QY-x2004    M17 神经母细胞瘤 DMEM+10%胎牛血清                      

QY-x2005    LAN-5 神经母细胞瘤 1640+10%胎牛血清                    

QY-x2006    LAN-6 神经母细胞瘤 1640+10%胎牛血清                    

QY-x2007    SK-N-SH 神经母细胞瘤 DMEM+10%胎牛血清                      

QY-x2008    SH-SY5Y 神经母细胞瘤 DMEM+10%胎牛血清                      

QY-x2009    SF126 脑瘤 1640+10%胎牛血清                    

QY-x2010    SF17 脑瘤 1640+10%胎牛血清                     

QY-x2011    SF763 脑瘤 瘤株                    

QY-x2012    SF767 脑瘤 瘤株                    

QY-x2013    MLA-144 长臂猿淋巴瘤 1640+10%胎牛血清                      

QY-x2014    T47D 乳腺导管癌 1640+15%胎牛血清                       

QY-x2015    ZR-75-1 乳腺导管癌 1640+10%胎牛血清                    

QY-x2016    A875 黑色素瘤 1640+10%胎牛血清                     

QY-x2017    SK-HEL-1 皮肤黑色素瘤                      

QY-x2018    A431 皮肤基底细胞癌                     【人胰激肽原酶(PK)ELISA试剂盒现货】

QY-x2019    Jurkat 急性T淋巴细胞白血病 1640+10%胎牛血清                       

QY-x2020    U937 单核细胞白血病 1640+10%胎牛血清                       

QY-x2021    MJ 淋巴瘤 1640+10%胎牛血清                     

QY-x2022    CEM 白血病 1640+10%胎牛血清                    

QY-x2023    K562 慢性髓原白血病 1640+10%胎牛血清                       

QY-x2024    HPB-ALL T细胞白血病 1640+10%胎牛血清                       

QY-x2025    HL-60 白血病 1640+10%胎牛血清                      

QY-x2026    Daudi B淋巴细胞瘤 1640+10%胎牛血清                     

QY-x2027    SK-BR-3 乳腺腺癌 1640+15%胎牛血清                      

QY-x2028    MCF-7 乳腺腺癌 1640+10%胎牛血清                    

QY-x2029    MCF-7/Adr 乳腺腺癌/阿霉素耐药株 1640+10%胎牛血清                       

QY-x2030    A2780 卵巢癌 1640+10%胎牛血清