检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供*优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【人间变性淋巴瘤激酶(ALK)ELISA试剂盒现货】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

【人间变性淋巴瘤激酶(ALK)ELISA试剂盒现货】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在、第二孔中分别加标准品100μl,然后在、第二孔中加标准品稀释液50μl,混匀；然后从孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品*终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

【人间变性淋巴瘤激酶(ALK)ELISA试剂盒现货】注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果.

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请*后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

【人间变性淋巴瘤激酶(ALK)ELISA试剂盒现货】计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

19588       Elution Microtubes CL (24 x 96)                 CNY                             

19133       QIAGEN Proteinase K (10)        10 ml (>600 mAU/ml, solution)  CNY                             

19076       Buffer ATL         200 ml Tissue Lysis Buffer for 1000 preps         CNY                             

19048       EndoFree Plasmid Buffer Set   Buffers P1, P2, P3, QBT, QC, QN, ER, TE, Endotoxin-free water, RNase A; for 10  plasmid mega- or 5 gigapreps (endotoxin-free)    CNY                             

12961       QIAGEN Plasmid Plus Maxi Sample Kit (5)        QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega ａnd Giga) or up to 24 (Midi ａnd Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction.      CNY                             

12243       QIAfilter Plasmid Midi Kit (25) 25 QIAGEN-tip 100, Reagents, Buffers, 25 QIAfilter Midi Cartridges      CNY                             

12123       QIAGEN Plasmid Mini Kit (25) 25 QIAGEN-tip 20, Reagents, Buffers      CNY                             

133276     NeXtalStock Spermine (100)             CNY                             

4503065  artus CMV LC PCR Kit (96) CE            CNY                             

6000-5000        Specimen Collection Tubes                CNY                              【人间变性淋巴瘤激酶(ALK)ELISA试剂盒现货】

5000-1430        MST Vortexer II - FoamFeetAssembly, 110V             CNY                             

5128-1220        Specimen Transport Medium (STM) CE             CNY                             

5123-1220        Female Swab Specimen Collection KitTM                  CNY                             

5100-1400IVD hc2 Sample Conversion Kit                CNY                             

EA11424  EASYartus BKV RG_II                 CNY                             

5050-1075 V.1 CD Rom Manual, MST Vortexer 2             CNY                             

EA10124  EASYartus EBV RG PCR Kit 2             CNY                             

BR-02       DxS B-RAF Mutation Test Kit            CNY                             

9240729  Tubing, 3/8"OD , Red, AP          CNY                             

9240719  Cable Carrier, Capper, Elevator, AP          CNY                             

9240701  O-Ring, Shaker, Lower Body, AP96/196             CNY                             

9240696  Fitting, Barbed, 1/4" ID tube, AP               CNY                             

9243651  Comprehen Valid., QIAgility     """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   For planning purposes ａnd to ensure a smooth process, the Customer is expected to have all materials on-hand ａnd inventoried one (1) week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """     CNY                             

218076     miScript SYBR Green PCR Kit (2000)                  CNY                             

271906     cattletype Milk Prep Kit (50)             CNY                             

289980     intype IC-DNA (1ml)                   CNY                              【人间变性淋巴瘤激酶(ALK)ELISA试剂盒现货】

280437     virotype BTV RT-PCR Kit (480)          CNY                             

932836     #QIAsymphony Ni-NTA Denat. Kit (384)           CNY                             

800511     Verification Sequencing / DNA production                CNY                             

9238243  ZLV BioRobot M96 User Manual               CNY                             

9019988  ZLV Mainboard, QGY                 CNY                             

9020553  Foam angle, transport box, QIAC              CNY                             

9020582  X-Motor, DML3000           CNY                             

QY-x2434 人肝癌细胞 Hep 3B2.1-7 询价 ATCC                                                         【人间变性淋巴瘤激酶(ALK)ELISA试剂盒现货】

QY-x2435 人宫颈癌细胞 Hela 询价 ATCC                                               

QY-x2436 大鼠心肌细胞 H9c2(2-1) 询价 ATCC                                                        

QY-x2437 人乳腺细胞 HBL-100 询价 ATCC                                                      

QY-x2438 人T淋巴细胞系 H9 询价 ATCC                                                        

QY-x2439 小鼠淋巴瘤细胞 EL-4 询价 ATCC                                                     

QY-x2440 人前列腺癌细胞 DU 145 询价 ATCC                                                        

QY-x2441 人成巨核细胞白血病细胞 Daudi 询价 ATCC                                                  

QY-x2442 非洲绿猴SV40转化的肾细胞 COS-7 询价 ATCC                                                    

QY-x2443 人结肠腺癌细胞 COLO 320 询价 ATCC                                                    

QY-x2444 人鼻咽癌细胞 CNE 询价 ATCC                                                

QY-x2445 人肺腺癌 (胸水) Calu-3 询价 ATCC                                                  

QY-x2446 人急性淋巴细胞白血病T淋巴细胞 CCRF-CEM 询价 ATCC                                                   

QY-x2447 人肾透明细胞癌皮肤转移细胞 Caki-1 询价 ATCC                                                        

QY-x2448 Burkitts淋巴癌细胞系 CA46 询价 ATCC                                                

QY-x2449 人宫颈癌肠转移细胞 Ca Ski 询价 ATCC                                                  

QY-x2450 大鼠胶质瘤细胞 C6 询价 ATCC                                                         【人间变性淋巴瘤激酶(ALK)ELISA试剂盒现货】

QY-x2451 人乳腺管癌细胞 BT-549 询价 ATCC                                                

QY-x2452 仓鼠幼仓鼠肾细胞 BHK-21 [C-13] 询价 ATCC                                                 

QY-x2453 人肝癌细胞 BEL-7402 询价 ATCC                                                  

QY-x2454 小鼠黑色素瘤细胞 B16 询价 ATCC                                                 

QY-x2455 人转移胰腺腺癌细胞 AsPC-1 询价 ATCC                                                

QY-x2456 人胚肾细胞 AAV-293 询价 ATCC                                                      

QY-x2457 人肺癌细胞 A549 [A-549] 询价 ATCC                                                       

QY-x2458 人表皮癌细胞 A-431 询价 ATCC                                                      

QY-x2459 人T淋巴细胞白血病细胞 A3 询价 ATCC                                                        

QY-x2460 人高转移肺癌细胞 95-D 询价 ATCC                                                        

QY-x2461 人肾透明细胞腺癌细胞 786-O 询价 ATCC                                                      

QY-x2462 小鼠脂肪细胞 3T3-L1 询价 ATCC                                                     

QY-x2463 人胚肾细胞 293T 询价 ATCC                                                   

QY-x2464 人膀胱癌细胞 5637 询价 ATCC