【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【人糖原合成酶激酶(GSK)ELISA试剂盒现货】样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【人糖原合成酶激酶(GSK)ELISA试剂盒现货】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

【人糖原合成酶激酶(GSK)ELISA试剂盒现货】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【人糖原合成酶激酶(GSK)ELISA试剂盒现货】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

52304     QIAamp RNA Blood Mini Kit (50) For 50 RNA preps: 50 QIAamp Mini Spin Columns, 50 QIAshredder Spin Columns, Collection Tubes (1.5 ml ａnd 2 ml), RNase-free Reagents ａnd Buffers      CNY                           

51306     QIAamp DNA Mini Kit (250) For 250 DNA preps: 250 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml)   CNY                           

9002012  Rotor-Gene Q MDx 2plex HRM Platform            CNY                           

970530    NRAS Pyro Kit (24)             CNY                           

970022    PyroMark Q24 CpG MLH1 (4 x 24)     For quantification of methylation level in region -209 to -188 from the transcription start site. CNY                           

969261    Ni-NTA Superflow 96 BioRobot Kit (4)        CNY                           

133199    NeXtalStock HEPES pH 8.0 (200)        CNY                            【人糖原合成酶激酶(GSK)ELISA试剂盒现货】

292143    mericon Horse Kit (24)         CNY                           

206047    Type-it Fast SNP Probe PCR Kit (4000)       "Features

 Outstanding separation for reliably high call rates

 For difficult SNP loci ａnd for low amounts of template

 Validated using TaqMan SNP Genotyping Assays

 Up to 40% time savings due to fast-cycling procedure

 Convenient ｍaster mix format ａnd optimized protocol"  CNY                           

292025    mericon Cattle (96)        CNY                           

291113    mericon RR Soy Kit (24)              CNY                           

9001908  QIAgility HEPA / UV (w/o PC) System QIAgility HEPA / UV (w/o PC) System combines the QIAgility instrument ａnd installation. The Installation plus Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.     CNY                           

133111    NeXtalStock Sodium fluoride (200)             CNY                           

9018540  Kit, installation, Qsym           CNY                           

9240672  Kerk Nut, Common, AP        CNY                           

9240658  Pump, Vacuum, Jun-Air, AP         CNY                           

9240642  Cable Assy, Proteinase K Pump, AP196/98          CNY                           

9240638  Opto Sensor, Reagent Pump, AP          CNY                           

9240617  Fuse, 2.0A, FNM, AP96        CNY                           

9240589  MAC Valve, 4-Way, Cent. Locator, AP96             CNY                           

9240560  Rotor/Buckets, Eppendorf, AP196/98          CNY                           

9240554  Bucket Grease, Eppendorf, AP196/98          CNY                           

9240523  Cable, Motor, Axis #0, Dispense, AP98        CNY                            【人糖原合成酶激酶(GSK)ELISA试剂盒现货】

9240507  Software & Std. Protocols, AP196              CNY                           

9240377  QIAcube, Installation     The Installation, QIAcube facilitates the quick implementation of the QIACube into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the QIAcube instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.    CNY                           

9239571  BR Universal, Basic Agreement     The Basic Cover Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.       CNY                           

9239570  BR Universal, Premium Agreement       The Premium Cover Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day). CNY                           

9020032  FOOTSET, RGQ           CNY                           

9019986  PCB, small breakout, 12 core, C12              CNY                           

9019479  UPPER BUFFER LID GS             CNY                           

9238864  BR M48, Premium Agreement       The Premium Cover Agreement M48 provides full service coverage for the BioRobot M48. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day). CNY                           

9238852  LiquiChip, Basic Agreement   The Basic Cover Agreement LiquiChip provides full service coverage for the LiquiChip workstation. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.       CNY                           

9241224  QIAxcel, Inspection Service  "Repair service delivery not applicable

Service response time (repair) not applicable

Periodic inspection/maintenance: yes, 1/agreement year

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: not included

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: Yes, included"       CNY                           

NG0138  植物样本提取液    100毫升 BR                       

NG0139  抗酸染液/Acid-faststain 50毫升×3     BR                       

NG0140  果糖测试盒    50管/48样     比色法                        

NG0141  微量丙二醛测试盒/MDA测试盒 50管/24样     比色法                         【人糖原合成酶激酶(GSK)ELISA试剂盒现货】

NG0142  细胞活性鉴别染液/Cellviabilitydetectedstain       20毫升   BR                       

NG0143  血管通透性测试染液/Vasopermeabilitystain 20毫升   BR                       

NG0144  过氧化物酶染液/Peroxidasestain  5毫升×3；2毫升×1  BR                       

NG0145  血清高密度脂蛋白胆固醇测试盒/HDL-C测试盒      80毫升   沉淀分离法手工                        

NG0146  血清低密度脂蛋白测试盒/LDL测试盒      80毫升   手工/半自动                       

NG0147  蔗糖测试盒    50管/48样     比色法                        

NG0148  蔗糖磷酸合成酶测试盒/SPS测试盒   50管/24样     比色法                        

NG0149  蔗糖合成酶测试盒/SS测试盒     50管/24样     比色法                        

NG0150  硝酸还原酶测试盒/NR测试盒    50管/24样     比色法                        

NG0151  ATP含量测试盒    100管/48样   比色法                        

NG0152  γ-谷氨酰胺半胱氨酸合成酶测试盒/γ-GCS测试盒       100管/48样   微量定磷法                        

NG0153  F0F1-ATP酶/ATP合成酶测试盒 20管/10样     生化法                        

NG0154  二胺氧化酶测试盒/DAO测试盒  25毫升/40T   手工比色法                        

NG0155  质粒提取试剂盒    100T/套  BR                       

NG0156  红细胞裂解液       100毫升 BR                       

NG0157  RIPA组织细胞快速裂解液  20毫升   BR                        【人糖原合成酶激酶(GSK)ELISA试剂盒现货】

NG0158  血糖测试盒/葡萄糖测试盒   96T 酶法                           

NG0159  丙氨酸氨基转移酶测试盒    60T PO法                         

NG0160  蛋白质羧基测试盒/蛋白质羰基测试盒      50管/24样     紫外比色法                        

NG0161  过氧化氢测试盒/H2O2测试盒    50管/48样     比色法                        

NG0162  铜兰蛋白测试盒/铜蓝蛋白测试盒/CP测试盒    50管/48样     比色法                        

NG0163  抗“O”胶乳测试盒     100T×2 BR                       

NG0164  类风湿因子胶乳试剂盒       100T×2 BR                       

NG0165  血氨测试盒    50管/48样     比色法                        

NG0166  乙醇脱氢酶测试盒       50管/48样     紫外比色法                        

NG0167  还原型谷胱甘肽+巯基测试盒/GSH+-SH测试盒       100管/48样   比色法                        

NG0168  DAB浓缩型试剂盒       3毫升     BR