Source Description
Aeromonas caviae
Unit Definition
One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA/Hind III fragments in 1 hour in a total reaction volume of 50 μl.
Reaction Conditions
Incubation at 37 °C
Reaction Buffer
20 mM Tris-Acetate (pH 7.5 at 37 °C)10 mM magnesium acetate50 mM potassium acetate1 mM dithiothreitol100 μg/ml bovine serum albumin
Storage Buffer
10 mM Tris-HCl (pH 7.5 at 4 °C) 1 mM dithiothreitol 50 mM KCl0.1 mM EDTA10 mM MgCl2200 μg/ml bovine serum albumin 50% (v/v) glycerol
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