Abcam抗体CD11b antibody [EP1345Y] ab52478

Product Name 

CD11b antibody [EP1345Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP1345Y] to CD11b

Immunogen 

Synthetic peptide corresponding to C terminus of human CD11b

Reacts with 

Hu

Does not react with 
（species key）

Ms, Rat

Specificity 

This antibody reacts with CD11b

Tested applications 
（see key）

Flow Cyt, ICC, IHC-P, IP, WB

Abreviews 

Customer reviews （see key）

Read about Abreviews

Application notes 
（see key）

Recommended dilutions
Flow Cyt: 1/20 - 1/50.
ICC: 1/100 - 1/250.
IHC-P: 1/100 - 1/250. 1/100 - 1/250. An antigen retrieval step is recommended.
IP: 1/80.
WB: 1/20,000 - 1/50,000. Predicted molecular weight: 128 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
（see definition）

IHC-P: Human spleen tissue
WB: TF1 lysate

Research areas 

Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> NK Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Murine Lineage Negative
Stem Cells >> Mesenchymal Stem Cells >> Negative Markers
Neuroscience >> Cell Type Marker >> Glia marker >> Microglia marker
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha

Target

Function 

Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages ａnd granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X ａnd ICAM1. It recognizes P1 ａnd P2 peptides of fibrinogen gamma chain.

Tissue specificity 

Predominantly expressed in monocytes ａnd granulocytes.