This product is for in vitro research only ａnd is not intended for use in humans or animals.
Description
Pyruvate kinase （PK） regulates the final rate-limiting step of glycolysis in the production of pyruvate andadenosine triphosphate （ATP）. Alternate splicing of PKM pre-mRNA leads to PKM2 generation by theinclusion of exon 10 ａnd the exclusion of exon 9, which is specific for PKM1. Besides its cytosolic roles inglycolysis, PKM2, which is upregulated by growth factor receptor activation 1, is phosphorylated at S37 byextracellular signal-regulated kinase （ERK） 2. This phosphorylation leads to the cis-trans isomerization ofPKM2 by the peptidyl-proline isomerase protein interactingwith never in mitosis A 1 （PIN1）, exposure of the nuclearlocalization signal （NLS） of PKM2, ａnd subsequent binding ofimportin 5 for nuclear translocation 2. In the nucleus, PKM2binds to phosphorylated Y333 of -catenin, which is essentialfor -catenin transactivation 3, ａnd interacts with andphosphorylates histone H3 at T11, leading to H3-K9acetylation ａnd transcription of genes such as MYC andCCND1 4. c-Myc expression results in the upregulation ofGLUT1, lactate dehydrogenase A （LDHA）, and, in a positivefeedback loop, PTB-dependent PKM2, which subsequentlyenhances the Warburg effect 2. Cyclin D1 expression, in turn,promotes G1-S phase transition 3, 4.Specificity: The antibody detects endogenous levels of total PKM2 protein.Formulation: 1.0 mg/mL in phosphate buffer without Mg2+/Ca2+, pH7.4, 150mM NaCl ａnd 50% glycerolDilution: 1:500 for Western BlottingSynonyms: PKM PK3 OIP3 PK2Accession: Swiss-Port #: P14618-1 NCBI Gene #: 5315 NCBI Protein #: NP_872270.1References
1 Yang W, Xia Y, Cao Y et al. EGFR-induced ａnd PKCepsilon monoubiquitylation-dependent NF-kappaBactivation upregulates PKM2 expression ａnd promotes tumorigenesis. Molecular cell 2012; 48:771-784.2 Yang W, Zheng Y, Xia Y et al. ERK1/2-dependent phosphorylation ａnd nuclear translocation of PKM2promotes the Warburg effect. Nature cell biology 2012; 14:1295-1304.3 Yang W, Xia Y, Ji H et al. Nuclear PKM2 regulates beta-catenin transactivation upon EGFR activation.
Nature 2011; 480:118-122.
4 Yang W, Xia Y, Hawke D et al. PKM2 phosphorylates histone H3 ａnd promotes gene transcription andtumorigenesis. Cell 2012; 150:685-696.
PKM2 Antibody
Catalog: #AA001 100 ul
Orders: gmxhwh@hotmail.com
Application Species Reactivity Source Storage
WB, IP Human Mouse Monoclonal Ab -20 C
上海国源生物技术有限公司成立于1999年，由多位生物学领域科学家和高级实验师参加，是一家以技术开发为背景，结合生产型企业。企业的宗旨是研究开发先进的生产技术和工艺 ，生产和销售符合国际同行业标准的产品；同时研制开发具有知识产权的新医药，新健康食品项目；从而更好地为人类健康事业作出贡献。
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上海国源生物技术有限公司至今已完成多项生物技术项目的开发与转让，有基因工程促红细胞生成素项目，基因工程人胰岛素项目，新型减肥药项目，上游基因工程实验平台技术等重要项目。目前在研项目涉及生物医药，保健食品，生化试剂，生物农药，新型安全饲料添加剂等多个领域。
