美国ABI TaqMan Genotyping Master Mix 4371353 4371355

 Excellent pre- ａnd post-PCR stability for high throughput setup ａnd analysis

May be used for a variety of genotyping applications, including:

 Candidate gene studies
 Drug-target validation 
 Disease association studies
 Population genetics
 Linkage mapping
 Agricultural applications

Reaction size is based on a 50 μl reaction volume; please see below for other reaction sizes.

Exceptional Performance from an Optimized Mix (2X)
Accurate genotype assignments result from preferential binding of the allele-specific probe to the matching target. TaqMan® Genotyping Master Mix enables specific binding of the probe to achieve exceptional cluster resolution. Key features include:

 AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure) – Automatic hot start enzyme designed to be active during thermal cycling ａnd inactive at room temperature for easy reaction setup. 
 Optimized mix components provide excellent specificity for discrimination between alleles 
 Passive internal reference based on proprietary ROX? dye for increased precision on Applied Biosystems® real-time PCR instruments 
 Single thermal cycling condition for consistent results with TaqMan® Assays

Validated with TaqMan® Genotyping Assays ａnd Instruments
TaqMan® Genotyping Master Mix has been tested across all types of TaqMan® SNP Genotyping Assays: TaqMan® Drug Metabolism Genotyping Assays, TaqMan® SNP Genotyping Assays, ａnd Custom TaqMan® SNP Genotyping Assays. In addition, the ｍaster mix is validated with the Applied Biosystems® thermal cyclers ａnd real-time PCR systems.

High-Throughput Setup ａnd Analysis
The combination of several low-cost thermal cyclers for PCR ａnd a single real-time PCR instrument for allelic discrimination make high throughput SNP genotyping manageable. TaqMan® Genotyping Master Mix further improves high-throughput setup ａnd analysis with excellent room temperature stability both before ａnd after PCR. This provides the flexibility required to run long experiments, unattended overnight or over a weekend. 

Reliable Discrimination with Challenging Targets
Amplicon design constraints make SNP detection challenging, particularly when targets contain an abundance of GC- or AT-rich regions. The optimized components of TaqMan® Genotyping Master Mix result in tight, well-separated clusters ａnd more accurate allele-calls compared to other mixes, even for challenging targets.

Setting the Industry Standard for Quality
Extensive analytical ａnd functional tests are performed for each manufactured lot of TaqMan® Genotyping Master Mix, including one TaqMan® SNP Genotyping Assay ａnd one TaqMan® Drug Metabolism Genotyping Assay. Results are compiled in an informative Certificate of Analysis, which is accessible through the Applied Biosystems® Web site (search for Certificate of Analysis).

Number of Genotyping Reactions at a Given Reaction Size:

4371355, TaqMan® Genotyping Master Mix, 1-Pack (1 x 10 mL)
 50 μL Reactions: 400
 20 μL Reactions: 1000
 10 μL Reactions: 2,000
 5 μL Reactions: 4,000