是用于分离和纯化抗体的通用性亲和层析介质之一。Protein A是一种分离自金黄色葡萄球菌的细胞壁蛋白，主要通过Fc片段结合哺乳动物IgG，但是不与狗lgG结合，不结合人lgM、lgD和IgA。天然Protein A有五个IgG结合区域和一些未知功能的区域，重组rprotein A去除了与白蛋白及细胞表面结合位点，只含有五个IgG结合区域，减少了非特异性吸附。
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