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选择齐一生物的原因:
1.供货及时。我们订货周期短,运输时间短,在最短时间内为您提供所需产品。
2.价格优惠:美国ATCC的中国代理商,没有中间环节。在国内我们保证价格最低。
3.服务保障:我们拥有专用的技术指导团队,对每售出的一件产品,提供全面的售前、售中和售后服务。
4.产品种类齐全:25个系统共150多种细胞以及各类菌株,满足不同客户的要求。细胞经过严格的质控,纯度可达98%。常用产品备有现货。其产品类型如下:消化系统细胞株,呼吸系统细胞株,泌尿系统细胞株生殖系统细胞株 血液循环系统 内皮细胞细胞株 神经系统及其他细胞株,动物细胞株和菌株
5.对于部分难培养的细胞我公司可代复苏细胞,并提供技术支持。
6.经验丰富:我公司是国内专业化的细胞销售公司,具有十余年细胞产品销售经验,有专业的销售团队和技术人员。齐一生物公司的每一位员工都将竭诚为您服务。
7.质量保证,保证售后
8. 齐一生物销售:021-6034 8496;181214 53965;173021 04490
【人心肌细胞】 齐一生物公司专业代理ScienCell简介ScienCell Research Laboratories (ScienCell) is a biotechnology company whose mission is the research and development of cell and cell-related products for experimental and therapeutic use. The ScienCell technology provides a variety of high quality normal human and animal cells, cell culture media and reagents, medium supplements, cell-derived RNA, cDNA and proteins for the research community. Scientists at ScienCell are also studying and developing cell therapeutic strategies that will significantly improve the quality of life by achieving the cell's ability to combat human degenerative disorders. We are based in San Diego, California and were established in 1999.
1. 接收到细胞,肉眼观察细胞培养基颜色,显微镜观察细胞生长情况,并对细胞进行不同倍数拍照(建议收到时的培养瓶拍一张照片,显微镜拍收到时的细胞100X,200X各一张)。
2.用75%的酒精消毒(建议配置75%的酒精的水是灭菌过的)。
3.严格无菌操作,打开细胞培养瓶。
4.将细胞培养瓶内的培养基用吸管完全吸出(严禁直接倾倒),放入另一无菌容器中(建议换新的培养基培养细胞)。
5.用PBS 2-3ml/次轻轻洗细胞表面,洗3-4次遍,加入0.05%胰酶-EDTA1-1.5ml,显微镜下观察细胞变圆,轻轻拍打培养瓶直到细胞脱落,加入终止液终止。
6.1000rpm,5min离心,重悬细胞。
7.换新的细胞培养瓶,37℃,5%CO2培养。【人心肌细胞】
齐一生物销售:021-6034 8496;181214 53965;173021 04490
9240713Poly Muffler Kit, AP96/196CNY
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9243654Comprehen Valid., Other Quant. Sys."""The Comprehensive Validation Support plan specific to a laboratory's needs will incorporate all of the requirements for the internal validation guidelines from the FBI's Quality Assurance Standards (QAS).
The Comprehensive Validation Support service includes all travel and labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, and amplify the validation study samples and may require multiple individuals. Usually, two weeks is required to analyze the data and one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.
Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform and complete all extractions, quantifications, amplfications, and capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.
Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation. Non-QIAGEN chemistry and accessories are not included and should be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) and required substrates (swabs, materials, etc). For planning purposes and to ensure a smooth process, the Customer is expected to have all materials on-hand and inventoried one (1) week prior to the validation start date.
Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) and STR (amp) analysis and linkage to starting samples.
Validation data CD/flashdrive – QIAGEN will review the validation data and draft a validation summary document, ensuring compliance with the FBI's QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review and signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.
Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation and generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager and the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """CNY
6000-5013LumiCheck Plate User PackageCNY
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For manual setup of hanging drop crystallization experiments."CNY
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203745QIAGEN Fast Cycling PCR Kit (1000)For 1000 x 20 µl reactions: 10 x 1 ml Fast Cycling Master Mix (2x), RNase-free water, 10 x CoralLoad Fast Cycling Dye, 5 x Q-SolutionCNY
203609HotStarTaq Plus DNA Polymerase (25000)100 x 250 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 100 x 2.0ml Q-Solution, 25 mM MgCl2CNY
201205Taq DNA Polymerase (1000)"4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2
NEW! Now with 10x CoralLoad PCR Buffer
Taq DNA Polymerase is supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity and minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye and a red dye, that facilitate estimation of DNA migration distance and optimization of agarose gel run time."CNY
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