A375人类恶性黑色素瘤 

选择齐一生物的原因：

1.供货及时。我们订货周期短，运输时间短，在最短时间内为您提供所需产品。

2.价格优惠：美国ATCC的中国代理商，没有中间环节。在国内我们保证价格最低。

3.服务保障：我们拥有专用的技术指导团队，对每售出的一件产品，提供全面的售前、售中和售后服务。

4.产品种类齐全：25个系统共150多种细胞以及各类菌株，满足不同客户的要求。细胞经过严格的质控，纯度可达98％。常用产品备有现货。其产品类型如下：消化系统细胞株，呼吸系统细胞株，泌尿系统细胞株生殖系统细胞株 血液循环系统 内皮细胞细胞株 神经系统及其他细胞株，动物细胞株和菌株

5.对于部分难培养的细胞我公司可代复苏细胞，并提供技术支持。

6.经验丰富：我公司是国内专业化的细胞销售公司，具有十余年细胞产品销售经验，有专业的销售团队和技术人员。齐一生物公司的每一位员工都将竭诚为您服务。

7.质量保证，保证售后

8. 齐一生物销售：021－6034 8496；181214 53965；173021 04490

【A375人类恶性黑色素瘤 】 齐一生物公司专业代理ScienCell简介ScienCell Research Laboratories (ScienCell) is a biotechnology company whose mission is the research ａnd development of cell ａnd cell-related products for experimental ａnd therapeutic use. The ScienCell technology provides a variety of high quality normal human ａnd animal cells, cell culture media ａnd reagents, medium supplements, cell-derived RNA, cDNA ａnd proteins for the research community. Scientists at ScienCell are also studying ａnd developing cell therapeutic strategies that will significantly improve the quality of life by achieving the cell＇s ability to combat human degenerative disorders. We are based in San Diego, California ａnd were established in 1999.

【A375人类恶性黑色素瘤 】美国ATCC简介：美国菌种保藏中心， 又称美国模式菌种收集中心(ATCC)，是位于马里兰洲洛克菲勒的一家私营的，非赢利性组织。目前它可以提供各种动植物细胞、标准品、菌株达两万多种。ATCC成立于1925年，是世界上最大的生物资源中心，由美国14家生化、医学类行业协会组成的理事会负责管理，是一家全球性、非盈利生物标准品资源中心。ATCC向全球发布其获取、鉴定、保存及开发的生物标准品，推动科学研究的验证、应用及进步 ATCC可以提供以下类别生物标准品：细胞株（3000多种）；菌株（15000多种）；动植物病毒株（2500多种）以及重组物质等。

现已成为可信赖的活体微生物、细胞系等获得、保存和发放的国家资源中心。ATCC有29,000多种不同品系可靠的动物细胞和微生物培养体，它能满足各科学团体对可信赖品系的需要。仅1981年就有39,000多培养物发放给全世界的科学工作者，并成功地用冷冻和冷冻干燥的方法保存各种细胞品系。 ATCC是一个非(学会单位)无利润的公司，由各科学组织推选成员组成理事会进行管理。现有工作人员125人。参加的科学团体有：美国免疫协会、美国生物化学会、美国细胞生物学会、美国微生物学会、美国动物学会、美国遗传学会、原生动物学会和组织培养协会等16个学术组织。在ATCC可利用各种材料以及工具和设备进行广泛的研究。主要研究项目有：酶联免疫吸附分析技术(ELISA)，真核细胞转录的调控，杂交瘤、淋巴类和髓类以及其他细胞系的建立，人突变细胞系的基因蛋白质，借助计算机进行鉴别分析，培养物的特征鉴别和保存技术的改进,关于化学致癌、致畸和致突变的。

【A375人类恶性黑色素瘤 】齐一生物提醒接收到细胞注意事项

1. 接收到细胞，肉眼观察细胞培养基颜色，显微镜观察细胞生长情况，并对细胞进行不同倍数拍照（建议收到时的培养瓶拍一张照片，显微镜拍收到时的细胞100X,200X各一张）。

2.用75%的酒精消毒（建议配置75%的酒精的水是灭菌过的）。

3.严格无菌操作，打开细胞培养瓶。

4.将细胞培养瓶内的培养基用吸管完全吸出（严禁直接倾倒），放入另一无菌容器中（建议换新的培养基培养细胞）。

5.用PBS 2-3ml/次轻轻洗细胞表面，洗3-4次遍，加入0.05%胰酶-EDTA1-1.5ml,显微镜下观察细胞变圆，轻轻拍打培养瓶直到细胞脱落，加入终止液终止。

6.1000rpm，5min离心，重悬细胞。

7.换新的细胞培养瓶，37℃，5%CO2培养。【A375人类恶性黑色素瘤 】

齐一生物销售：021－6034 8496；181214 53965；173021 04490

9001910QIAgility HEPA/UV (w/o PC) PrioPLUS"QIAgility HEPA/UV (w/o PC)  Instrument 

Installation of QIAgility instrument & Introductory training 

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite pipetting head service ａnd inspection per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"CNY

133116NeXtalStock Sodium thiocyanate (200)CNY

967142RNeasy 96 BioRobot 9604 Kit (12)For 12 x 96 total ａnd cytoplasmic RNA preps on the BioRobot 9604: 12 RNeasy 96 Plates, Elution Microtubes CL (1.2 ml), Caps, Square-Well Blocks, RNase-free Reagents ａnd BuffersCNY

962141QIAprep 96 Turbo BioRobot Kit (4)For 4 x 96 high-purity plasmid minipreps, 4 each: TurboFilter 96 ａnd QIAprep 96 Plates; Flat-Bottom Blocks ａnd Lids, Reagents, Buffers, Collection Microtubes (1.2 ml) ａnd Caps, 96-Well Microplates RB ａnd Lids, Tape PadsCNY

960241QIAGEN Plasmid Plus 96 BioRobot Kit (4)CNY

51304QIAamp DNA Mini Kit (50)For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml)CNY

978756PyroMark CpG Assay 96 well (200)CNY

9012712Z-motor (Plato 7) leftCNY

9012696Set of X-flat band cable (Plato 7)CNY

133239NeXtalStock Sodium citrate pH 7.0 (200)CNY

133238NeXtalStock Sodium citrate pH 5.8 (200)CNY

9002127QIAsymphony RGQ, PriorityQIAsymphony RGQ Priority includes the QIAsymphony RGQ instruments, installations ａnd extended warranties. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.CNY

59203EpiTect Whole Bisulfitome Kit (25)CNY

59164EpiTect Plus LyseAll Bisulfite Kit (48)CNY

929526QX Alignment Marker 50 bp/1 kb (1.5 ml)CNY

133218NeXtalStock MOPS pH 8.0 (200)CNY

301605RNAiFect Transfection Reagent (1ml)RNAiFect Reagent ａnd buffer, for up to 170 transfections in 24-well plates; up to 500 transfections in 96-well platesCNY

301525TransMessenger Tranfect. Reagent(0.5ml)For 60 transfections in 6-well plates or 80 transfections in 12-well platesCNY

52304QIAamp RNA Blood Mini Kit (50)For 50 RNA preps: 50 QIAamp Mini Spin Columns, 50 QIAshredder Spin Columns, Collection Tubes (1.5 ml ａnd 2 ml), RNase-free Reagents ａnd BuffersCNY

51306QIAamp DNA Mini Kit (250)For 250 DNA preps: 250 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml)CNY

9002012Rotor-Gene Q MDx 2plex HRM PlatformCNY

970530NRAS Pyro Kit (24)CNY

970022PyroMark Q24 CpG MLH1 (4 x 24)For quantification of methylation level in region -209 to -188 from the transcription start site.CNY

969261Ni-NTA Superflow 96 BioRobot Kit (4)CNY

133199NeXtalStock HEPES pH 8.0 (200)CNY

292143mericon Horse Kit (24)CNY

206047Type-it Fast SNP Probe PCR Kit (4000)"Features

 Outstanding separation for reliably high call rates

 For difficult SNP loci ａnd for low amounts of template

 Validated using TaqMan SNP Genotyping Assays 

 Up to 40% time savings due to fast-cycling procedure

 Convenient ｍaster mix format ａnd optimized protocol"CNY

292025mericon Cattle (96)CNY

291113mericon RR Soy Kit (24)CNY

9001908QIAgility HEPA / UV (w/o PC) SystemQIAgility HEPA / UV (w/o PC) System combines the QIAgility instrument ａnd installation. The Installation plus Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.CNY