碱性成纤维细胞生长因子刺激的小鼠胚胎成纤维细胞条件培养基

选择齐一生物的原因：

1.供货及时。我们订货周期短，运输时间短，在最短时间内为您提供所需产品。

2.价格优惠：美国ATCC的中国代理商，没有中间环节。在国内我们保证价格最低。

3.服务保障：我们拥有专用的技术指导团队，对每售出的一件产品，提供全面的售前、售中和售后服务。

4.产品种类齐全：25个系统共150多种细胞以及各类菌株，满足不同客户的要求。细胞经过严格的质控，纯度可达98％。常用产品备有现货。其产品类型如下：消化系统细胞株，呼吸系统细胞株，泌尿系统细胞株生殖系统细胞株 血液循环系统 内皮细胞细胞株 神经系统及其他细胞株，动物细胞株和菌株

5.对于部分难培养的细胞我公司可代复苏细胞，并提供技术支持。

6.经验丰富：我公司是国内专业化的细胞销售公司，具有十余年细胞产品销售经验，有专业的销售团队和技术人员。齐一生物公司的每一位员工都将竭诚为您服务。

7.质量保证，保证售后

8. 齐一生物销售：021－6034 8496；181214 53965；173021 04490

【碱性成纤维细胞生长因子刺激的小鼠胚胎成纤维细胞条件培养基】 齐一生物公司专业代理ScienCell简介ScienCell Research Laboratories (ScienCell) is a biotechnology company whose mission is the research ａnd development of cell ａnd cell-related products for experimental ａnd therapeutic use. The ScienCell technology provides a variety of high quality normal human ａnd animal cells, cell culture media ａnd reagents, medium supplements, cell-derived RNA, cDNA ａnd proteins for the research community. Scientists at ScienCell are also studying ａnd developing cell therapeutic strategies that will significantly improve the quality of life by achieving the cell＇s ability to combat human degenerative disorders. We are based in San Diego, California ａnd were established in 1999.

【碱性成纤维细胞生长因子刺激的小鼠胚胎成纤维细胞条件培养基】美国ATCC简介：美国菌种保藏中心， 又称美国模式菌种收集中心(ATCC)，是位于马里兰洲洛克菲勒的一家私营的，非赢利性组织。目前它可以提供各种动植物细胞、标准品、菌株达两万多种。ATCC成立于1925年，是世界上最大的生物资源中心，由美国14家生化、医学类行业协会组成的理事会负责管理，是一家全球性、非盈利生物标准品资源中心。ATCC向全球发布其获取、鉴定、保存及开发的生物标准品，推动科学研究的验证、应用及进步 ATCC可以提供以下类别生物标准品：细胞株（3000多种）；菌株（15000多种）；动植物病毒株（2500多种）以及重组物质等。

现已成为可信赖的活体微生物、细胞系等获得、保存和发放的国家资源中心。ATCC有29,000多种不同品系可靠的动物细胞和微生物培养体，它能满足各科学团体对可信赖品系的需要。仅1981年就有39,000多培养物发放给全世界的科学工作者，并成功地用冷冻和冷冻干燥的方法保存各种细胞品系。 ATCC是一个非(学会单位)无利润的公司，由各科学组织推选成员组成理事会进行管理。现有工作人员125人。参加的科学团体有：美国免疫协会、美国生物化学会、美国细胞生物学会、美国微生物学会、美国动物学会、美国遗传学会、原生动物学会和组织培养协会等16个学术组织。在ATCC可利用各种材料以及工具和设备进行广泛的研究。主要研究项目有：酶联免疫吸附分析技术(ELISA)，真核细胞转录的调控，杂交瘤、淋巴类和髓类以及其他细胞系的建立，人突变细胞系的基因蛋白质，借助计算机进行鉴别分析，培养物的特征鉴别和保存技术的改进,关于化学致癌、致畸和致突变的。

【碱性成纤维细胞生长因子刺激的小鼠胚胎成纤维细胞条件培养基】齐一生物提醒接收到细胞注意事项

1. 接收到细胞，肉眼观察细胞培养基颜色，显微镜观察细胞生长情况，并对细胞进行不同倍数拍照（建议收到时的培养瓶拍一张照片，显微镜拍收到时的细胞100X,200X各一张）。

2.用75%的酒精消毒（建议配置75%的酒精的水是灭菌过的）。

3.严格无菌操作，打开细胞培养瓶。

4.将细胞培养瓶内的培养基用吸管完全吸出（严禁直接倾倒），放入另一无菌容器中（建议换新的培养基培养细胞）。

5.用PBS 2-3ml/次轻轻洗细胞表面，洗3-4次遍，加入0.05%胰酶-EDTA1-1.5ml,显微镜下观察细胞变圆，轻轻拍打培养瓶直到细胞脱落，加入终止液终止。

6.1000rpm，5min离心，重悬细胞。

7.换新的细胞培养瓶，37℃，5%CO2培养。【碱性成纤维细胞生长因子刺激的小鼠胚胎成纤维细胞条件培养基】

齐一生物销售：021－6034 8496；181214 53965；173021 04490

9241825Pyro Q96 MDa, Full AgreementThe Full Agreement, PyM provides full service coverage for the PyroMark instrument. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one on-site inspection service, with costs for labor ａnd parts covered. With a Full Agreement, there is a response time of 2 working days.CNY

9241167Board, LED scan, QXCNY

9241148Transport lock, Y axis, M48CNY

9241143Application AUTOPURE (customized)CNY

9241005QIArent&go - Rent out fee BR UniversalCNY

9240987Kit, electronic, FFC-plugs, QsymCNY

9240980Sensor, tray, sample tracking, QsymCNY

9240945Sensor, xy, portal, QsymCNY

9234856Tubing coupling piece for washer liquidCNY

9234852Card, ISDN, Fritz PCICNY

9240783Update, Weigh Cell, AP96CNY

9240774Assy, Transfer Rack, AP96CNY

9241632LED Light System, APCNY

9241369Temperature Controller, EZ1aCNY

9243645Guided Valid Support, QIA Extraction SysThe QIAGEN Other Extraction System Guided Validation Support service includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to QIAGEN extraction chemistry utilized in the process, review the appropriate Handbook ａnd User Manual, as well as hands-on chemistry ａnd instrument operation as required.  The Guided Validation service includes experimental design consultation, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support. Please indicate which protocols to validate (Biosprint 96, DNA mericon)CNY

9243542BR MDx, PM Service"Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"CNY

9243305Power Cord, IndiaCNY

9243110CD-RW, Black, AP196/98CNY

9242712Validation Support, 2d QIAcubeThe QIAGEN QIAcube Guided Validation includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist. A QIAGEN specialist will provide an introduction to the QIAcube system ａnd the DNA Investigator silica chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on QIAcube operation.  The Guided Validation service includes experimental design consulation, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support.  Please indicate which protocols to include in the validation (QIAamp, QIAamp DNA Investigator, MinElute PCR Cleanup, etc.)CNY

9019226Fan - rear 92x92, PyroQ24CNY

9019220PCB - Distribution robot, PyroQ96MDCNY

1066843PG Influenza A RT-PCR Assay (48) LE ZCNY

1066848NDV Univ. RT-PCR REAGENT LECNY

1066854AIV-H9 RT-PCR REAGENT LECNY

1060992Buffer MW1 (77ml)CNY

1061059Sample Preparation Reagents, 8mlCNY

1061080NG Quantification StandardsCNY

1061113PG AIV-H7 RT-PCR Assay (48) ZCNY

9016568Plate Grippers, RCS1CNY

9016536Transportation box , BioSprint 15CNY

9016535Transportation box , Twister IICNY