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4.产品种类齐全:25个系统共150多种细胞以及各类菌株,满足不同客户的要求。细胞经过严格的质控,纯度可达98%。常用产品备有现货。其产品类型如下:消化系统细胞株,呼吸系统细胞株,泌尿系统细胞株生殖系统细胞株 血液循环系统 内皮细胞细胞株 神经系统及其他细胞株,动物细胞株和菌株
5.对于部分难培养的细胞我公司可代复苏细胞,并提供技术支持。
6.经验丰富:我公司是国内专业化的细胞销售公司,具有十余年细胞产品销售经验,有专业的销售团队和技术人员。齐一生物公司的每一位员工都将竭诚为您服务。
7.质量保证,保证售后
8. 齐一生物销售:021-6034 8496;181214 53965;173021 04490
【重组人成纤维细胞生长因子--碱性】 齐一生物公司专业代理ScienCell简介ScienCell Research Laboratories (ScienCell) is a biotechnology company whose mission is the research and development of cell and cell-related products for experimental and therapeutic use. The ScienCell technology provides a variety of high quality normal human and animal cells, cell culture media and reagents, medium supplements, cell-derived RNA, cDNA and proteins for the research community. Scientists at ScienCell are also studying and developing cell therapeutic strategies that will significantly improve the quality of life by achieving the cell's ability to combat human degenerative disorders. We are based in San Diego, California and were established in 1999.
1. 接收到细胞,肉眼观察细胞培养基颜色,显微镜观察细胞生长情况,并对细胞进行不同倍数拍照(建议收到时的培养瓶拍一张照片,显微镜拍收到时的细胞100X,200X各一张)。
2.用75%的酒精消毒(建议配置75%的酒精的水是灭菌过的)。
3.严格无菌操作,打开细胞培养瓶。
4.将细胞培养瓶内的培养基用吸管完全吸出(严禁直接倾倒),放入另一无菌容器中(建议换新的培养基培养细胞)。
5.用PBS 2-3ml/次轻轻洗细胞表面,洗3-4次遍,加入0.05%胰酶-EDTA1-1.5ml,显微镜下观察细胞变圆,轻轻拍打培养瓶直到细胞脱落,加入终止液终止。
6.1000rpm,5min离心,重悬细胞。
7.换新的细胞培养瓶,37℃,5%CO2培养。【重组人成纤维细胞生长因子--碱性】
齐一生物销售:021-6034 8496;181214 53965;173021 04490
211033QuantiTect Virus +ROX Vial Kit (200)CNY
210215QIAGEN OneStep RT-PCR Kit (1000)CNY
9012760Magnetic Stirrer, 2 positionCNY
800201Single Read HT, w/ prepCNY
800176Easy-Read Plates, w/o prep"Sequencing of plasmid DNA or PCR products
DNA quality control not included
No repetition of sequencing
Data collected to maximal read length
Minimum order at least 96 reads
One primer per plate"CNY
800090Ready-Run Rxns.CNY
765312PAXgene TissueFIX Cont. (50 ml) KitCNY
132009EasyXtal 15-Well Tool X-Seal (100)"100 empty 15-well crystallization plates with X-Seal crystallization supports (5 boxes of 20)
For manual setup of hanging drop crystallization experiments."CNY
762931Buffer BM3 Wash buffer 8 x 150 ml conc.CNY
204054QuantiFast SYBR Green PCR Kit (400)CNY
203741QIAGEN Fast Cycling PCR Kit (50)"Trial Kit: For 50 x 20 µl reactions: 1 x 0.5 ml Fast Cycling Master Mix (2x), RNase-free water, 10 x CoralLoad Fast Cycling Dye, 5 x Q-Solution
-to be used as sample Kit"CNY
159340Sterile Foam-Tipped Swabs (100)CNY
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35061Ni-NTA HisSorb Plates (5)5 Ni-NTA-coated, transparent 96-well platesCNY
678891ipsogen MLL-DUP e2e8 StandardsCNY
678591ipsogen MLL-AF9 e8e10 StandardsCNY
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32746EasyXpress Linear Template Fab Kit (100)CNY
32501EasyXpress Protein Synthesis Kit (5)"- Kit for cell-free protein expression in E.coli cell-free extracts.
- The same as the EasyXpress Protein Synthesis Kit (20) (former EasyXpress Protein Synthesis Mini Kit), but only 5 instead of 20 reactions
- Ideal for testing and expression of QIAgenes"CNY
30900Glutathione HiCap Matrix (10 ml)CNY
28181QIAquick 96 PCR Purification Kit (4)For purification of 4 x 96 PCR reactions: 4 QIAquick 96 Plates, Buffers, Collection Microtubes (1.2 ml), CapsCNY
9011595Tip adapter, 300 µl, 120 mm, v2.04CNY
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9013174Connector, tubing, 1-8, BR8000CNY
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9011433BGR Z-Motor P1300 Spez. MWGCNY
9011422Tip Rack Holder, RCS1CNY
9243119Green Laser Contr. Coherent 115M-13, LCCNY
9243556HC2, Full Agreement"Service response time (on-site repair):not applicable
Periodic inspection/maintenance: yes, 1/agreement year
Cost coverage depot repair (parts, labor, packaging, shipping): yes, full coverage
For defect DML, after customer calling QIAGEN technical service hotline before 12 am:
- A special package containing a replacement unit shall be sent to the customer within 2 working days.
- Customer shall send the defect unit to QIAGEN Regional Repair Center following the instructions attached with the packaging.
For DML owned by customer, QIAGEN Regional Repair Center will send back the repaired original unit to customers within 7-10 working days.
PC, Monitor, Heater, Shaker, Washer, Printer and Votexer:
- A new unit shall be sent to customer within 7-10 working days at no extra cost
- Customer shall send the defect unit to QIAGEN for disposal
- On-site installation of PC and application software is not covered by the agreement
The Preventive Maintenance (PM) service ensures the correct operation of the equipment. During the PM, all the instrument modules are inspected by a qualified QIAGEN service technician. The Preventive Maintenance is performed under the manufacturer guidelines and documented according to GLP / GMP"CNY
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